Effects of injectable trace minerals on the immune response to Mannheimia haemolytica and Pasteurella multocida following vaccination of dairy calves with a commercial attenuated-live bacterin vaccine

2018 ◽  
Vol 34 (1) ◽  
pp. 59-66 ◽  
Author(s):  
J.H.J. Bittar ◽  
D.J. Hurley ◽  
A.R. Woolums ◽  
N.A. Norton ◽  
C.E. Barber ◽  
...  
Keyword(s):  
Immune Response ◽  
Pasteurella Multocida ◽  
Mannheimia Haemolytica ◽  
Dairy Calves ◽  
Trace Minerals

scholarly journals Isolation and Identification of Pasteurella multocida from Chicken for the Preparation of Oil Adjuvanted Vaccine

2013 ◽  
Vol 2 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Samina Ievy ◽  
Mohammad Ferdousur Rahman Khan ◽  
Md Ariful Islam ◽  
Md Bahanur Rahman
Keyword(s):  
Immune Response ◽  
Pasteurella Multocida ◽  
Challenge Infection ◽  
Cholera Vaccine ◽  
Isolation And Identification ◽  
Passive Hemagglutination ◽  
Fowl Cholera ◽  
Adjuvanted Vaccine ◽  
Group A ◽  
The Mean

The research work was performed for the isolation and identification of Pasteurella multocida from field cases, preparation of oil adjuvanted vaccine from isolated strain and determination of its efficacy. Samples were collected from suspected dead birds of three poultry farms of Bangladesh (Code name: M and R). The P. multocida isolates were Gram negative, non-motile, non- spore forming rod occurring singly or pairs and occasionally as chains or filaments. Biochemically P. multocida ferment basic sugar and consistently produced acid except from maltose and lactose. After isolation formalin killed oil adjuvanted Fowl cholera vaccine was prepared in Laboratory of the Department of Microbiology and Hygiene, BAU and this experimental vaccine (3.2x108 CFU/ml) was administered in nine weeks old White Leg Horn chickens at the different dose rate through intramuscular (IM) route in each selected group A (1ml alum precipitated vaccine), B (0.5ml alum precipitated vaccine), C (1ml oil adjuvanted vaccine) and D (0.5ml oil adjuvanted vaccine). Pre-vaccinated sera were collected from all groups of birds. The mean of Passive Hemagglutination (PHA) titers of post-vaccination were 51±17.8, 76.8±17, 89.6±17, and 115±17.81 in group A, B, C and D respectively which consist of 5 birds in each. The vaccine produced better immune response when boostering with the similar dose and route at 15 days after primary vaccination. The mean PHA titers were higher at group D than other groups after boostering. Challenge infection was conducted on all the vaccinated and control group (n=5) of birds after 15 days of vaccination which protect 93.75% of birds and the PHA titers from different groups analyzed to determine the protective capacity of vaccinated chickens against challenge exposure. It was demonstrated that experimental oil adjuvanted fowl cholera vaccine with 0.5ml dose produce higher immune response against challenge infection and found to be safe. Microbes and Health, June 2013, 2(1): 1-4DOI: http://dx.doi.org/10.3329/mh.v2i1.17253

scholarly journals Intranasal Bacterial Therapeutics Reduce Colonization by the Respiratory Pathogen Mannheimia haemolytica in Dairy Calves

mSystems ◽  
2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Samat Amat ◽  
Trevor W. Alexander ◽  
Devin B. Holman ◽  
Timothy Schwinghamer ◽  
Edouard Timsit
Keyword(s):  
Total Mortality ◽  
Mannheimia Haemolytica ◽  
Dairy Calves ◽  
Respiratory Pathogen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Rrna Gene ◽  
Nasal Colonization ◽  
Content Type ◽  
Nasal Microbiota

ABSTRACT Six Lactobacillus strains originating from the nasopharyngeal microbiota of cattle were previously characterized in vitro and identified as candidate bacterial therapeutics (BTs) for mitigating the bovine respiratory pathogen Mannheimia haemolytica. In the present study, these BT strains were evaluated for their potential to (i) reduce nasal colonization by M. haemolytica, (ii) modulate the nasal microbiota, and (iii) stimulate an immune response in calves experimentally challenged with M. haemolytica. Twenty-four Holstein bull calves (1 to 3 weeks old) received either an intranasal BT cocktail containing 6 Lactobacillus strains (3 × 109 CFU per strain; BT + Mh group) 24 h prior to intranasal M. haemolytica challenge (3 × 108 CFU) or no BTs prior to challenge (Mh, control group). Nasal swab, blood, and transtracheal aspiration samples were collected over the course of 16 days after BT inoculation. Counts of M. haemolytica were determined by culturing, and the nasal and tracheal microbiotas were evaluated using 16S rRNA gene sequencing. Serum cytokines (interleukin-6 [IL-6], IL-8, and IL-10) were quantified by enzyme-linked immunosorbent assay (ELISA). Administration of BT reduced nasal colonization by M. haemolytica (P = 0.02), modified the composition and diversity of the nasal microbiota, and altered interbacterial relationships among the 10 most relatively abundant genera. The BT + Mh calves also had a lower relative abundance of Mannheimia in the trachea (P < 0.01) but similar cytokine levels as Mh calves. This study demonstrated that intranasal BTs developed from the bovine nasopharyngeal Lactobacillus spp. were effective in reducing nasal colonization by M. haemolytica in dairy calves. IMPORTANCE Bovine respiratory disease (BRD) is one of the significant challenges for the modern dairy industry in North America, accounting for 23 to 47% of the total mortality among pre- and postweaned dairy heifers. Mass medication with antibiotics is a common practice to control BRD in dairy cattle. However, the emergence of multidrug-resistant BRD pathogens highlights the importance of developing alternatives to antibiotics for BRD mitigation. Using a targeted approach, we recently identified 6 Lactobacillus strains originating from the bovine respiratory microbiota as candidates to be used as bacterial therapeutics (BTs) for the mitigation of the BRD pathogen Mannheimia haemolytica. Here, we demonstrated that intranasal inoculation of the BT strains reduced nasal colonization by M. haemolytica in dairy calves experimentally challenged with this pathogen. This study, for the first time, shows the potential use of intranasal BTs as an alternative to mitigate BRD pathogens in cattle.

scholarly journals PSVI-25 Antimicrobial resistance profiles of Pasteurella multocida, Mannheimia haemolytica and Moraxella spp. of bovine origin pre- and post- treatment with antimicrobials in calves

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 212-212
Author(s):  
Lourdes Migura-Garcia ◽  
Sonia Marti ◽  
Anna Aris ◽  
Ana Perez de Rozas ◽  
Carolina Tejero ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Bronchoalveolar Lavage Fluid ◽  
Clinical Laboratory ◽  
Lavage Fluid ◽  
Mannheimia Haemolytica ◽  
Resistant Bacteria ◽  
Respiratory Pathogens ◽  
Resistant Species ◽  
Current Production ◽  
Clinical Breakpoints

Abstract The current production system involves movement of calves combined with mixing animals, favouring the transmission of respiratory pathogens and increasing the risk of using antimicrobials. The aim of the study was to determine the presence and resistant profiles of Pasteurella multocida, Mannheimia haemolytica and Moraxella in the lungs of calves at arrival to the farm and after treatment with tulathromycin and florfenicol. Thoracic ultrasonography was performed in two batches of calves (n = 65 and 120, age=26±11.0, weight=45-55kg) at arrival, to select 27 calves/batch with lesions from low to severe. Bronchoalveolar lavage fluid (BALF) was collected on arrival (D0), D20 and D34, and also on D7 for the second batch. Pasteurella, Mannheimia and Moraxella were identified by VITEK. Detection of Mycoplasma bovis was performed by PCR. Minimal inhibitory concentration (MIC) was determined for 18 antimicrobials. Clinical breakpoints were defined by Clinical Laboratory Standards Institute. ERIC-PCR was performed in all isolates to assess similarities. Time effect was analysed by MIXED Procedure (SAS). At D0, 16 out of 54 BALFs contained P. multocida (n = 11), M. haemolytica (n = 5) and Moraxella (n = 4). The number of calves positive for primary pathogens decreased (P &lt; 0.001) at D7 and D20, whereas by D34 positive animals leapt up to 21. MIC results demonstrated that P. multocida and M. haemolytica obtained at D0 were pansusceptible. Isolates of P. multocida collected after treatment, exhibited resistance to oxytetracycline, tilmicosin, tulathromycin, danofloxacin and enrofloxacin. ERIC-PCR demonstrated similar profiles of P. multocida and Moraxellain the two batches of calves after D7. Primary pathogens were susceptible to the initial treatment, however M. bovis was detected in all animals after D7. Resistant species appeared to recolonize the lungs after D34. ERIC-profile demonstrated that isolates recovered after treatment were different from those colonizing the lungs at arrival, suggesting recirculation of resistant bacteria between batches.

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