scholarly journals IS6100-mediated genetic rearrangement within the complex class 1 integron In104 of the Salmonella genomic island 1

2010 ◽  
Vol 65 (7) ◽  
pp. 1543-1545 ◽  
Author(s):  
H. Targant ◽  
B. Doublet ◽  
F. M. Aarestrup ◽  
A. Cloeckaert ◽  
J.-Y. Madec
Keyword(s):  
Genomic Island ◽  
Complex Class ◽  
Class 1 Integron ◽  
Class 1 ◽  
Genetic Rearrangement

scholarly journals Truncated tni Module Adjacent to the Complex Integron of Salmonella Genomic Island 1 in Salmonella enterica Serovar Virchow

2008 ◽  
Vol 53 (2) ◽  
pp. 824-827 ◽  
Author(s):  
Benoît Doublet ◽  
Chishih Chu ◽  
Cheng-Hsun Chiu ◽  
Yi-Chin Fan ◽  
Axel Cloeckaert
Keyword(s):  
Multidrug Resistance ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Open Reading Frame ◽  
Complex Class ◽  
Class 1 Integron ◽  
Reading Frame ◽  
Class 1 ◽  
First Time

ABSTRACT Salmonella genomic island 1 was identified for the first time in Salmonella enterica serovar Virchow isolated from humans in Taiwan. The complex class 1 integron conferring multidrug resistance was shown to be inserted within open reading frame (ORF) S023 and contains for the first time a partial transpositional module. The 5-bp target duplication flanking the complex integron suggests that its insertion in ORF S023 was by transposition.

scholarly journals Salmonella Genomic Island 1 Multidrug Resistance Gene Clusters in Salmonella enterica Serovar Agona Isolated in Belgium in 1992 to 2002

2004 ◽  
Vol 48 (7) ◽  
pp. 2510-2517 ◽  
Author(s):  
Benoît Doublet ◽  
Patrick Butaye ◽  
Hein Imberechts ◽  
David Boyd ◽  
Michael R. Mulvey ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Gene Clusters ◽  
Complex Class ◽  
Class 1 Integron ◽  
Transposase Gene ◽  
New Variant ◽  
Class 1

ABSTRACT Salmonella genomic island 1 (SGI1) harbors a multidrug resistance (MDR) gene cluster which is a complex class 1 integron. Variant SGI1 MDR gene clusters conferring different MDR profiles have also been identified in several Salmonella enterica serovars and classified as SGI1-A to -F. A retrospective study was undertaken to characterize MDR regions from serovar Agona strains harboring SGI1 isolated from poultry in Belgium between 1992 and 2002. A total of 171 serovar Agona strains, displaying resistance to at least one antibiotic, were studied for the presence of SGI1. SGI1 was detected in 94 serovar Agona strains. The most prevalent variant was SGI1-A (85%), which harbors within the SGI1 complex class 1 integron a common region (CR1) containing orf513, a putative transposase gene, adjacent to the dfrA10 trimethoprim resistance gene. A new variant SGI1 named SGI1-G was identified in two strains. It consisted of the pse-1 gene cassette, as in SGI1-B, but with additional insertion of the orf513/dfrA10 region structure. Seven strains displaying the typical SGI1 MDR profile (Ap Cm Ff Sm Sp Su Tc) showed genetic variation at the 3′ end of SGI1. These strains harbored the insertion of the CR1 containing orf513 as in SGI1-A, -D, and -G. However, downstream the right end of CR1, they presented different 7.4- to 8.5-kb deletions of the SGI1 3′ end that extended to the chromosomal genes yieE and yieF. These results suggest a possible role of CR1 in deletion formation, as has been reported for some insertion sequences. Pulsed-field gel electrophoresis analysis showed that all the serovar Agona SGI1-carrying strains belonged to a single clone. Thus, SGI1 is largely encountered in serovar Agona strains isolated from poultry in Belgium, the most prevalent variant being SGI1-A. SGI1 MDR region undergoes recombinational events resulting in a diversity of MDR gene clusters.

scholarly journals PGI2 Is a Novel SGI1-Relative Multidrug-Resistant Genomic Island Characterized in Proteus mirabilis

2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Chang-Wei Lei ◽  
Yan-Peng Chen ◽  
Ling-Han Kong ◽  
Jin-Xin Zeng ◽  
Yong-Xiang Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Proteus Mirabilis ◽  
Genomic Island ◽  
Multidrug Resistant ◽  
Complex Class ◽  
Helper Plasmid ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1

ABSTRACT A novel 61,578-bp genomic island named Proteus genomic island 2 (PGI2) was characterized in Proteus mirabilis of swine origin in China. The 23.85-kb backbone of PGI2 is related to those of Salmonella genomic island 1 and Acinetobacter genomic island 1. The multidrug resistance (MDR) region of PGI2 is a complex class 1 integron containing 14 different resistance genes. PGI2 was conjugally mobilized in trans to Escherichia coli in the presence of a conjugative IncC helper plasmid.

Carriage of qnrA1 and qnrB2, blaCTX-M15, and complex class 1 integron in a clinical multiresistant Citrobacter freundii isolate

2010 ◽  
Vol 67 (2) ◽  
pp. 188-190 ◽  
Author(s):  
Sónia Ferreira ◽  
Ana Paradela ◽  
Jorge Velez ◽  
Elmano Ramalheira ◽  
Timothy R. Walsh ◽  
...  
Keyword(s):  
Citrobacter Freundii ◽  
Complex Class ◽  
Class 1 Integron ◽  
Class 1

scholarly journals Salmonella genomic island 1 and class 1 integron in Salmonella isolates from stray dogs

2011 ◽  
Vol 5 (23) ◽  
pp. 3907-3912
Author(s):  
Chang Yi Chih ◽  
Chuang Hsiao Li ◽  
Chiu Chien Chao ◽  
Yeh Kuang Sheng ◽  
Chang Chao Chin ◽  
...  
Keyword(s):  
Genomic Island ◽  
Class 1 Integron ◽  
Stray Dogs ◽  
Class 1

scholarly journals Complex Class 1 Integron in a Clinical Escherichia coli Strain From Vietnam Carrying Both mcr-1 and blaNDM–1

2019 ◽  
Vol 10 ◽  
Author(s):  
Hong-Ngoc Le-Vo ◽  
Phuong Thi-Bich Tran ◽  
Lien Le ◽  
Yuki Matsumoto ◽  
Daisuke Motooka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coli Strain ◽  
Complex Class ◽  
Class 1 Integron ◽  
Class 1

scholarly journals SGI1-K, a Variant of the SGI1 Genomic Island Carrying a Mercury Resistance Region, in Salmonella enterica Serovar Kentucky

2006 ◽  
Vol 51 (1) ◽  
pp. 317-323 ◽  
Author(s):  
Renee S. Levings ◽  
Sally R. Partridge ◽  
Steven P. Djordjevic ◽  
Ruth M. Hall
Keyword(s):  
Salmonella Enterica ◽  
Genomic Island ◽  
Mercury Resistance ◽  
Variant Form ◽  
Short Segment ◽  
Class 1 Integron ◽  
Antibiotic Resistant ◽  
Right Hand ◽  
Class 1 ◽  
The Right

ABSTRACT A multiple-antibiotic-resistant Salmonella enterica serovar Kentucky strain was found to contain SGI1-K, a variant form of the Salmonella genomic island 1 (SGI1) with an In4-type class 1 integron that contains only one cassette array, aacCA5-aadA7, and an adjacent mercury resistance module. Part of the 3′-conserved segment (3′-CS) of the integron, together with the inverted short segment from the right-hand end of the integron transposition module normally found between the 3′-CS and IS6100 in In4 family integrons, has been removed by an IS6100-mediated deletion. IRt, the right-hand inverted repeat found at the outer end of the integron, abuts a mercury resistance region instead of the usual SGI1 backbone segment. The mer module is a hybrid of those found in Tn501 and Tn21. This mer region and a further uncharacterized segment of at least 10 kb appear to have been incorporated between IRt and the SGI1 backbone. These findings demonstrate that the multidrug resistance region in SGI1 can incorporate new DNA segments in the same way as multiple antibiotic resistance regions in plasmids.

scholarly journals qnrVC-Like Gene Located in a Novel Complex Class 1 Integron Harboring the ISCR1 Element in an Aeromonas punctata Strain from an Aquatic Environment in Shandong Province, China

2010 ◽  
Vol 54 (8) ◽  
pp. 3471-3474 ◽  
Author(s):  
Ruirui Xia ◽  
Xianhu Guo ◽  
Yuzhen Zhang ◽  
Hai Xu
Keyword(s):  
Amino Acid ◽  
Gene Cassette ◽  
Multidrug Resistant ◽  
Amino Acid Identity ◽  
Complex Class ◽  
Class 1 Integron ◽  
Class 1 ◽  
Gyrase A ◽  
Ciprofloxacin Resistance ◽  
Novel Complex

ABSTRACT A qnrVC-like gene, qnrVC4, was found in a novel complex class 1 integron gene cassette array following the ISCR1 element and bla PER-1 in a multidrug-resistant strain of the aquatic bacterium Aeromonas punctata. The deduced QnrVC4 protein sequence shares 45% to 81% amino acid identity with quinolone resistance determinants QnrB6, QnrA1, QnrS1, QnrC, QnrVC1, and QnrVC3. A Ser-83 to Ile amino acid substitution in gyrase A may be mainly responsible for ciprofloxacin resistance in this strain.

scholarly journals Early Strains of Multidrug-Resistant Salmonella enterica Serovar Kentucky Sequence Type 198 from Southeast Asia Harbor Salmonella Genomic Island 1-J Variants with a Novel Insertion Sequence

2012 ◽  
Vol 56 (10) ◽  
pp. 5096-5102 ◽  
Author(s):  
Simon Le Hello ◽  
François-Xavier Weill ◽  
Véronique Guibert ◽  
Karine Praud ◽  
Axel Cloeckaert ◽  
...  
Keyword(s):  
Southeast Asia ◽  
Salmonella Enterica ◽  
Insertion Sequence ◽  
Genomic Island ◽  
Far East ◽  
Gene Clusters ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1

ABSTRACTSalmonellagenomic island 1 (SGI1) is a 43-kb integrative mobilizable element that harbors a great diversity of multidrug resistance gene clusters described in numerousSalmonella entericaserovars and also inProteus mirabilis. The majority of SGI1 variants contain an In104-derivative complex class 1 integron inserted between resolvase generesand open reading frame (ORF) S044 in SGI1. Recently, the international spread of ciprofloxacin-resistantS. entericaserovar Kentucky sequence type 198 (ST198) containing SGI1-K variants has been reported. A retrospective study was undertaken to characterize ST198S. Kentucky strains isolated before the spread of the epidemic ST198-SGI1-K population in Africa and the Middle East. Here, we characterized 12 ST198S. Kentucky strains isolated between 1969 and 1999, mainly from humans returning from Southeast Asia (n= 10 strains) or Israel (n= 1 strain) or from meat in Egypt (n= 1 strain). All these ST198S. Kentucky strains did not belong to the XbaI pulsotype X1 associated with the African epidemic clone but to pulsotype X2. SGI1-J subgroup variants containing different complex integrons with a partial transposition module and inserted within ORF S023 of SGI1 were detected in six strains. The SGI1-J4 variant containing a partially deleted class 1 integron and thus showing a narrow resistance phenotype to sulfonamides was identified in two epidemiologically unrelated strains from Indonesia. The four remaining strains harbored a novel SGI1-J variant, named SGI1-J6, which containedaadA2,floR2,tetR(G)-tetA(G), andsul1resistance genes within its complex integron. Moreover, in all theseS. Kentucky isolates, a novel insertion sequence related to the IS630family and named ISSen5was found inserted upstream of the SGI1 complex integron in ORF S023. Thus, two subpopulations ofS. Kentucky ST198 independently and exclusively acquired the SGI1 during the 1980s and 1990s. Unlike the ST198-X1 African epidemic subpopulation, the ST198-X2 subpopulation mainly from Asia harbors variants of the SGI1-J subgroup that are encountered mainly in the Far East, as previously described forS. entericaserovars Emek and Virchow.

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