scholarly journals Rapid decline in the efficiency of HIV drug resistance genotyping from dried blood spots (DBS) and dried plasma spots (DPS) stored at 37 C and high humidity

2009 ◽  
Vol 64 (1) ◽  
pp. 33-36 ◽  
Author(s):  
J. G. Garcia-Lerma ◽  
A. McNulty ◽  
C. Jennings ◽  
D. Huang ◽  
W. Heneine ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Dried Blood Spots ◽  
Rapid Decline ◽  
High Humidity ◽  
Hiv Drug Resistance ◽  
Blood Spots ◽  
Drug Resistance Genotyping

Dried blood spots for HIV-1 drug resistance genotyping in decentralized settings in Senegal

2013 ◽  
Vol 86 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Abou Abdallah Malick Diouara ◽  
Halimatou Diop-Ndiaye ◽  
Khady Kebe-Fall ◽  
Edmond Tchiakpè ◽  
Ousseynou Ndiaye ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Drug Resistance Genotyping ◽  
Hiv 1

scholarly journals HIV-1 drug resistance genotyping from dried blood spots stored for 1 year at 4 C

2008 ◽  
Vol 61 (6) ◽  
pp. 1217-1220 ◽  
Author(s):  
A. S. Youngpairoj ◽  
S. Masciotra ◽  
C. Garrido ◽  
N. Zahonero ◽  
C. de Mendoza ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Drug Resistance Genotyping ◽  
Hiv 1

scholarly journals Pyrosequencing Dried Blood Spots Reveals Differences in HIV Drug Resistance between Treatment Naïve and Experienced Patients

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56170 ◽  
Author(s):  
Hezhao Ji ◽  
Yang Li ◽  
Binhua Liang ◽  
Richard Pilon ◽  
Paul MacPherson ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Dried Blood Spots ◽  
Hiv Drug Resistance ◽  
Blood Spots ◽  
Treatment Naïve

High concordance between HIV-1 drug resistance genotypes generated from plasma and dried blood spots in antiretroviral-experienced patients

AIDS ◽  
2007 ◽  
Vol 21 (18) ◽  
pp. 2503-2511 ◽  
Author(s):  
Silvina Masciotra ◽  
Carolina Garrido ◽  
Ae S Youngpairoj ◽  
Amanda McNulty ◽  
Natalia Zahonero ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
High Concordance ◽  
Hiv 1

scholarly journals A MiSeq-HyDRA platform for enhanced HIV drug resistance genotyping and surveillance

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Tracy Taylor ◽  
Emma R. Lee ◽  
Mikaela Nykoluk ◽  
Eric Enns ◽  
Binhua Liang ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Hiv Drug Resistance ◽  
Drug Resistance Genotyping

scholarly journals HIV-2 Drug Resistance Genotyping from Dried Blood Spots

2020 ◽  
Vol 59 (1) ◽  
pp. e02303-20
Author(s):  
Dana N. Raugi ◽  
Robert S. Nixon ◽  
Sally Leong ◽  
Khadim Faye ◽  
Jean Phillipe Diatta ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Viral Load ◽  
Plasma Viral Load ◽  
Mononuclear Cells ◽  
Dried Blood Spots ◽  
Antiretroviral Drugs ◽  
Resistance Testing ◽  
Peripheral Blood Mononuclear ◽  
Blood Spots ◽  
Drug Resistance Testing

ABSTRACTThe treatment of HIV-2 in resource-limited settings (RLS) is complicated by the limited availability of HIV-2-active antiretroviral drugs and inadequate access to HIV-2 viral load and drug resistance testing. Dried blood spots (DBS)-based drug resistance testing, widely studied for HIV-1, has not been reported for HIV-2 and could present an opportunity to improve care for HIV-2-infected individuals. We selected 150 DBS specimens from ongoing studies of antiretroviral therapy (ART) for HIV-2 infection in Senegal and subjected them to genotypic drug resistance testing. Total nucleic acid was extracted from DBS, reverse transcribed, PCR amplified, and analyzed by population-based Sanger sequencing, and major drug resistance-associated mutations (RAM) were identified. Parallel samples from plasma and peripheral blood mononuclear cells (PBMC) were also genotyped. We obtained 58 protease/reverse transcriptase genotypes. Plasma viral load was significantly correlated with genotyping success (P < 0.001); DBS samples with corresponding plasma viral load >250 copies/ml had a success rate of 86.8%. In paired DBS-plasma genotypes, 83.8% of RAM found in plasma were also found in DBS, and replicate DBS genotyping revealed that a single test detected 86.7% of known RAM. These findings demonstrate that DBS-based genotypic drug resistance testing for HIV-2 is feasible and can be deployed in RLS with limited infrastructure.

Minority HIV mutation detection in dried blood spots indicates high specimen integrity and reveals hidden archived drug resistance

2011 ◽  
Vol 50 (2) ◽  
pp. 148-152 ◽  
Author(s):  
Xierong Wei ◽  
Ae S. Youngpairoj ◽  
Carolina Garrido ◽  
Natalia Zahonero ◽  
Angelica Corral ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mutation Detection ◽  
Dried Blood Spots ◽  
Blood Spots
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