Subtype variability, virological response and drug resistance assessed on dried blood spots collected from HIV patients on antiretroviral therapy in Angola

2008 ◽  
Vol 61 (3) ◽  
pp. 694-698 ◽  
Author(s):  
C. Garrido ◽  
N. Zahonero ◽  
D. Fernandes ◽  
D. Serrano ◽  
A. R. Silva ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antiretroviral Therapy ◽  
Virological Response ◽  
Dried Blood Spots ◽  
Hiv Patients ◽  
Blood Spots

scholarly journals Drug resistance in HIV patients with virological failure or slow virological response to antiretroviral therapy in Ethiopia

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Alemseged Abdissa ◽  
Daniel Yilma ◽  
Jannik Fonager ◽  
Anne M Audelin ◽  
Lone H Christensen ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antiretroviral Therapy ◽  
Virological Failure ◽  
Virological Response ◽  
Hiv Patients

HIV type-1 drug resistance testing on dried blood spots is feasible and reliable in patients who fail antiretroviral therapy in rural Tanzania

2010 ◽  
Vol 15 (7) ◽  
pp. 1003-1009 ◽  
Author(s):  
Asgeir Johannessen ◽  
Mona Holberg-Petersen ◽  
Gunilla Lövgaarden ◽  
Ezra Naman ◽  
Vidar Ormaasen ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antiretroviral Therapy ◽  
Dried Blood Spots ◽  
Resistance Testing ◽  
Blood Spots ◽  
Drug Resistance Testing ◽  
Rural Tanzania ◽  
Hiv Type 1

Virological Response and Drug Resistance 1 and 2 Years Post-Partum in HIV-Infected Women Initiated on Life-Long Antiretroviral Therapy in Malawi

2016 ◽  
Vol 32 (8) ◽  
pp. 737-742 ◽  
Author(s):  
Sandro Mancinelli ◽  
Clementina Maria Galluzzo ◽  
Mauro Andreotti ◽  
Giuseppe Liotta ◽  
Haswel Jere ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antiretroviral Therapy ◽  
Virological Response ◽  
Post Partum

scholarly journals Virological Response and Antiretroviral Drug Resistance Emerging during Antiretroviral Therapy at Three Treatment Centers in Uganda

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0145536 ◽  
Author(s):  
Pontiano Kaleebu ◽  
Wilford Kirungi ◽  
Christine Watera ◽  
Juliet Asio ◽  
Fred Lyagoba ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antiretroviral Therapy ◽  
Virological Response ◽  
Antiretroviral Drug Resistance ◽  
Antiretroviral Drug

Dried blood spots for HIV-1 drug resistance genotyping in decentralized settings in Senegal

2013 ◽  
Vol 86 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Abou Abdallah Malick Diouara ◽  
Halimatou Diop-Ndiaye ◽  
Khady Kebe-Fall ◽  
Edmond Tchiakpè ◽  
Ousseynou Ndiaye ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Drug Resistance Genotyping ◽  
Hiv 1

High concordance between HIV-1 drug resistance genotypes generated from plasma and dried blood spots in antiretroviral-experienced patients

AIDS ◽  
2007 ◽  
Vol 21 (18) ◽  
pp. 2503-2511 ◽  
Author(s):  
Silvina Masciotra ◽  
Carolina Garrido ◽  
Ae S Youngpairoj ◽  
Amanda McNulty ◽  
Natalia Zahonero ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
High Concordance ◽  
Hiv 1

scholarly journals Adherence, virological outcome, and drug resistance in Chinese HIV patients receiving first-line antiretroviral therapy from 2011 to 2015

Medicine ◽  
2018 ◽  
Vol 97 (50) ◽  
pp. e13555 ◽  
Author(s):  
Pengtao Liu ◽  
Lingjie Liao ◽  
Wei Xu ◽  
Jing Yan ◽  
Zhongbao Zuo ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antiretroviral Therapy ◽  
First Line ◽  
Hiv Patients ◽  
Virological Outcome

scholarly journals HIV-2 Drug Resistance Genotyping from Dried Blood Spots

2020 ◽  
Vol 59 (1) ◽  
pp. e02303-20
Author(s):  
Dana N. Raugi ◽  
Robert S. Nixon ◽  
Sally Leong ◽  
Khadim Faye ◽  
Jean Phillipe Diatta ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Viral Load ◽  
Plasma Viral Load ◽  
Mononuclear Cells ◽  
Dried Blood Spots ◽  
Antiretroviral Drugs ◽  
Resistance Testing ◽  
Peripheral Blood Mononuclear ◽  
Blood Spots ◽  
Drug Resistance Testing

ABSTRACTThe treatment of HIV-2 in resource-limited settings (RLS) is complicated by the limited availability of HIV-2-active antiretroviral drugs and inadequate access to HIV-2 viral load and drug resistance testing. Dried blood spots (DBS)-based drug resistance testing, widely studied for HIV-1, has not been reported for HIV-2 and could present an opportunity to improve care for HIV-2-infected individuals. We selected 150 DBS specimens from ongoing studies of antiretroviral therapy (ART) for HIV-2 infection in Senegal and subjected them to genotypic drug resistance testing. Total nucleic acid was extracted from DBS, reverse transcribed, PCR amplified, and analyzed by population-based Sanger sequencing, and major drug resistance-associated mutations (RAM) were identified. Parallel samples from plasma and peripheral blood mononuclear cells (PBMC) were also genotyped. We obtained 58 protease/reverse transcriptase genotypes. Plasma viral load was significantly correlated with genotyping success (P < 0.001); DBS samples with corresponding plasma viral load >250 copies/ml had a success rate of 86.8%. In paired DBS-plasma genotypes, 83.8% of RAM found in plasma were also found in DBS, and replicate DBS genotyping revealed that a single test detected 86.7% of known RAM. These findings demonstrate that DBS-based genotypic drug resistance testing for HIV-2 is feasible and can be deployed in RLS with limited infrastructure.

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