scholarly journals Emerging bacterial pathogens: Escherichia coli, Enterobacter aerogenes and Proteus mirabilis clinical isolates harbouring the same transferable plasmid coding for metallo-β-lactamase VIM-1 in Greece

2007 ◽  
Vol 59 (3) ◽  
pp. 578-579 ◽  
Author(s):  
Irene Galani ◽  
Maria Souli ◽  
Evangelos Koratzanis ◽  
George Koratzanis ◽  
Zoi Chryssouli ◽  
...  
Author(s):  
T. O. Olowomofe ◽  
J. O. Oluyege ◽  
A. O. Ogunlade ◽  
D. T. Makinde

Bed linen is clearly recognized as a potential reservoir for microorganisms and could be a vector of disease transmission. The present study was aimed at isolating and characterizing bacteria and fungi from different kinds of bed linen of student in Ekiti State University hostels. Pour plate method was used for the enumeration of total bacteria count from the posterior and anterior end of the bed linen. The average bacteria count for the anterior and posterior end was 7.46 and 7.16 Log10 CFU/ml respectively. The most dominant microbial species were bacteria and these were mostly found in the environment and on human skin. The bacteria isolated were Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis, Enterobacter aerogenes, Bacillus cereus, Escherichia coli and Klebsiella aerogenes. Bacillus cereus had the highest frequency of occurrence (25%), followed by Staphylococcus aureus (15%), Escherichia coli (15%), Klebsiella aerogenes (15%), Staphylococcus epidermis (15%), Enterobacter aerogenes (10%) and Proteus mirabilis (5%). The fungi isolates were Aspergillus sulphureus, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus and Fusarium spp. Antibiotics susceptibility test was carried out on the bacteria isolates with gram negative bacteria showing resistance to Cotrimoxazole and gram positive bacteria showing resistance to Amplicillin. Most of the bacteria isolates have multiple antibiotics resistance. The bacterial isolates were susceptible to Ciprofloxacin. Plasmid profiling was also done with Escherichia coli having three high molecular weight plasmids, Bacillus cereus and Enterobacter aerogenes do not possess plasmid. The identified species are suspected to be opportunistic pathogens for human, representing a risk for people with weakened immune system, especially in cases of super-infection.


2020 ◽  
Vol 3 (2) ◽  
pp. 96
Author(s):  
Isna Romadhona ◽  
Fauna Herawati ◽  
Rika Yulia

Antibiotik merupakan obat yang digunakan untuk mengatasi dan mencegah infeksi bakteri. Penggunaan antibiotik yang tidak tepat dapat menimbulkan berbagai masalah, diantaranya pengobatan akan lebih mahal dan juga risiko terjadinya resistensi bakteri terhadap antibiotik. Penelitian ini bertujuan untuk mengetahui profil penggunaan antibiotik dan profil peta kuman pada pasien gangren diabetes melitus di sebuah RSUD di Kabupaten Gresik serta untuk mengetahui kesesuaian penggunaan antibiotik dengan mengacu pada Permenkes Republik Indonesia No. 2406/Menkes/PER/XII/2011. Data penggunaan antibiotik diperoleh dari catatan Rekam Medis pada periode Januari – November 2017. Data penggunaan antibiotik dihitung dengan menggunakan rumus DDD/100 pasien-hari rawat. Hasil perhitungan DDD/100 pasien-hari rawat menunjukkan hasil sebesar 470,11 DDD/100 pasien-hari rawat. Peta kuman pada pasien gangren, melaporkan adanya bakteri Enterobacter cloacae 24%, Escherichia coli 18%, Staphylococcus aureus 15%, Acinetobacter baumannii 9%, Pseudomonas aeruginosa 6%, Citrobacter youngae 6%, Enterobacter aerogenes 6%, Proteus vulgaris 6%, Staphylococcus schleiferi 6%, Klebsiella pneumoniae 3%, dan Proteus mirabilis 3% . Penggunaan antibiotik seftriakson dan metronidazol pada pasien gangren diabetes melitus di sebuah RSUD di Kabupaten Gresik pada periode Januari – November 2017 telah sesuai dengan pedoman penggunaan antibiotik berdasarkan Permenkes Republik Indonesia No. 2406/Menkes/PER/ XII/2011, yaitu antibiotik golongan sefalosporin generasi III yang lebih aktif terhadap Enterobacteriaceae dan antibiotik golongan nitroimidazol yang dapat mengobati infeksi bakteri basil anerob Gram-Negatif.


2020 ◽  
Vol 51 (4) ◽  
pp. 1807-1812
Author(s):  
Gabrielli Stefaninni Santiago ◽  
Daniela Gonçalves ◽  
Irene da Silva Coelho ◽  
Shana de Mattos de Oliveira Coelho ◽  
Helena Neto Ferreira

Author(s):  
ADENILDE RIBEIRO NASCIMENTO ◽  
JOÃO ELIAS MOUCHREK FILHO ◽  
SILVIO CARVALHO MARINHO ◽  
ANDRÉ GUSTAVO LIMA DE ALMEIDA MARTINS ◽  
MARTHA REIS SOUSA ◽  
...  

O objetivo deste trabalho foi avaliar as condições higiênico-sanitárias de diversos tipos de polpas de frutas comercializadas nas feiras livres de São Luís-MA, Brasil. Realizaram-se as análises de coliformes a 45ºC, Salmonella sp., Staphylococcus coagulase positiva e bolores e leveduras em 40 amostras adquiridas em feiras livres. Os resultados mostraram ausência de Salmonella sp. e Staphylococcus coagulase positiva nas alíquotas das amostras, respectivamente, 25 mL e 0,1 mL. Porém, verificou-se 100% de contaminação por bolores e leveduras e 12% por coliformes a 45°C. Algumas cepas de enterobactérias patogênicas ao homem foram identificadas, dentre as quais Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Citrobacter freundii, Erwinia uredovora e Proteus mirabilis.


2002 ◽  
Vol 2 (2) ◽  
pp. 174
Author(s):  
Kleiber Pessoa Borges ◽  
Aparecido Osdimir Bertolin

O Córrego São João tem sua nascente situada na Fazenda Pilão, no município de Porto Nacional-TO, abrange várias propriedades rurais, e na zona urbana alguns bairros representativos, como: Jardim Querido, Jardim Umuarama, Santa Helena e Vila Nova, desembocando no Rio Tocantins. Tanto em zonas rurais como urbanas, o rio recebe contribuições de resíduos poluidores (químicos, físicos e biológicos), no entanto, suas águas vêm sendo utilizadas principalmente para abastecimento público e irrigação. Mediante a amostragem sistemática, em pontos estratégicos ao longo do córrego, fez-se avaliação da qualidade da água, por meio de indicador bacteriano. As amostras coletadas foram analisadas microbiologicamente pelas técnicas de Tubos Múltiplos e Membrana Filtrante; e meios de cultura específicos para Enterobacteriaceae. Os valores mínimos e máximos encontrados para coliformes totais (CT) e coliformes fecais (CF) por 100 mL foram respectivamente: 0,17 x 102 - 660,0 x 102; 2,0 x 102 - 300,0 x 102. Foram isoladas e identificadas as espécies: Escherichia coli, Proteus mirabilis, Citrobacter diversus, Citrobacter freundii, Enterobacter aerogenes, Serratia odorifera, Salmonella sp e Edwardsiella tarda nos diferentes pontos de coleta. Concluiu-se que as águas do Córrego São João são impróprias para consumo humano, sem prévia desinfecção e para irrigação de hortas e outros tipos de alimentos consumidos pela população.


2019 ◽  
Vol 2 ◽  
Author(s):  
Anggi Reza Pramesti ◽  
Sella Mustika ◽  
Nurul Habibah ◽  
Sofia Puspitarini ◽  
Meichica Serlie ◽  
...  

Banyaknya kegiatan Penambangan Emas Tanpa Ijin (PETI) di Indonesia menjadi sumber utama terjadinya pencemaran limbah merkuri. Merkuri merupakan salah satu jenis polutan logam berat yang bersifat toksik. Merkuri menimbulkan masalah serius pada kesehatan manusia karena dapat terakumulasi pada tubuh dan bersifat neurotoksin. Beberapa bakteri memiliki kemampuan resisten terhadap merkuri. Sejumlah bakteri yang resisten terhadap limbah merkuri telah diisolasi dari berbagai lokasi pertambangan emas di Indonesia. Beberapa bakteri lokal tersebut dirangkum dalam penelitian ini. Bakteri tersebut diantaranya yaitu : Bacillus sp., Escherichia coli, Enterobacter cloacea, Enterobacter aerogenes, Klebsiella pneumoniae, Alcaligenes sp., Pseudomonas sp., Enterobacter agglomerans, Proteus vugaris, Proteus mirabilis, Enterobacter hafniae, Kliebsella sp., Lactobacillus sp., Morganella morganii, Brevundimonas vesicularis, Nitrococcus mobilis, Fusobacterium aquatile, dan Fusobacterium necrogenes. Bakteri yang resisten terhadap merkuri tersebut dapat dimanfaatkan sebagai agen untuk remediasi wilayah yang tercemar limbah merkuri


2000 ◽  
Vol 44 (1) ◽  
pp. 213-216 ◽  
Author(s):  
Helene Marchandin ◽  
Helene Jean-Pierre ◽  
Christophe De Champs ◽  
Danielle Sirot ◽  
Helene Darbas ◽  
...  

ABSTRACT Several Pseudomonas aeruginosa strains, including one urinary isolate producing an extended-spectrum β-lactamase TEM-24, were isolated from a long-term-hospitalized woman. Three TEM-24-producing enterobacterial species (Enterobacter aerogenes, Escherichia coli, and Proteus mirabilis) were isolated from the same patient. TEM-24 and the resistance markers for aminoglycosides, chloramphenicol, and sulfonamide were encoded by a 180-kb plasmid transferred by conjugation into E. coli HB101.


2012 ◽  
Vol 3 (1) ◽  
pp. 111-117
Author(s):  
A. O. Ponedilok ◽  
V. G. Gavryliuk ◽  
Y. V. Khlopova ◽  
A. I. Vinnikov

The spectrum of causative agents of nonspecific infections of the women urogenital tracts is studied. It is established that the typical etiological agents of the vaginosis are yeast-like fungi Candida albicans (35.7 %) and Escherichia coli (30.2 %), and the clinical isolates of E. coli (47.3 %) and Proteus mirabilis (15.8 %) are usual for vulvovaginitis. The frequency of detection of the causative agents of inflammatory genito-urinary diseases in women of different age groups varies: strains of E. coli are often found in patients of 1–12 years (47.3 %) and in women of 43–66 years old (36.0 %), but C. albicans – in patients of 18–42 years (39.0 %). High levels of the resistance to penicilline, tetracycline and fluoroquinolone antibiotics in selected clinical isolates of opportunistic microorganisms are determined. 


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