scholarly journals Variant Salmonella genomic island 1 antibiotic resistance gene clusters in Salmonella enterica serovar Derby isolates from humans in Taiwan

2006 ◽  
Vol 59 (2) ◽  
pp. 325-326 ◽  
Author(s):  
C.-H. Chiu ◽  
H.-L. Chen ◽  
L.-S. Kao ◽  
C.-Y. Yang ◽  
C. Chu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Antibiotic Resistance Gene ◽  
Gene Clusters

scholarly journals Variant Salmonella Genomic Island 1-L Antibiotic Resistance Gene Cluster in Salmonella enterica Serovar Newport

2006 ◽  
Vol 50 (11) ◽  
pp. 3944-3946 ◽  
Author(s):  
Axel Cloeckaert ◽  
Karine Praud ◽  
Benoît Doublet ◽  
Marie Demartin ◽  
François-Xavier Weill
Keyword(s):  
Antibiotic Resistance ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Antibiotic Resistance Gene ◽  
Gene Cassette ◽  
Class 1 Integron ◽  
Resistance Gene Cluster ◽  
Class 1

ABSTRACT We report a new Salmonella genomic island 1 variant antibiotic resistance gene cluster called SGI1-L in a Salmonella enterica serovar Newport isolate containing a dfrA15 gene cassette conferring resistance to trimethoprim. The isolate carried another class 1 integron containing the aacC5 and aadA7 gene cassettes conferring resistance to gentamicin and streptomycin/spectinomycin, respectively.

scholarly journals Variant Salmonella Genomic Island 1 Antibiotic Resistance Gene Cluster Containing a Novel 3′-N-Aminoglycoside Acetyltransferase Gene Cassette, aac(3)-Id, in Salmonella enterica Serovar Newport

2004 ◽  
Vol 48 (10) ◽  
pp. 3806-3812 ◽  
Author(s):  
Benoît Doublet ◽  
François-Xavier Weill ◽  
Laetitia Fabre ◽  
Elisabeth Chaslus-Dancla ◽  
Axel Cloeckaert
Keyword(s):  
Antibiotic Resistance ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Antibiotic Resistance Gene ◽  
Gene Cassette ◽  
Multidrug Resistant ◽  
Resistance Gene Cluster ◽  
New Variant

ABSTRACT Salmonella genomic island 1 (SGI1) harbors an antibiotic resistance gene cluster and was previously identified in the multidrug-resistant Salmonella enterica serovars Typhimurium DT104, Agona, Paratyphi B, and Albany. This antibiotic resistance gene cluster is a complex class 1 integron and most often confers resistance to ampicillin (Ap), chloramphenicol (Cm)/florfenicol (Ff), streptomycin (Sm)/spectinomycin (Sp), sulfonamides (Su), and tetracycline (Tc) (ApCmFfSmSpSuTc profile). Recently, variant SGI1 antibiotic resistance gene clusters conferring different antibiotic resistance profiles have been identified in several S. enterica serovars and were classified as SGI1-A to -G. We identified a new variant SGI1 antibiotic resistance gene cluster in two multidrug-resistant S. enterica serovar Newport strains isolated from humans in France. In these strains, the Sm/Sp resistance gene cassette aadA2 inserted at the first attI1 site was replaced by two other aminoglycoside resistance gene cassettes. The first one contains a new resistance gene encoding an AAC(3)-I aminoglycoside 3-N-acetyltransferase that confers resistance to gentamicin (Gm) and sisomicin (Sc). This gene has been named aac(3)-Id. The second one harbors the Sm/Sp resistance gene aadA7. This gene cassette replacement in the SGI1 complex integron of serovar Newport strains constitutes a new variant SGI1 antibiotic resistance gene cluster named SGI1-H. The occurrence of SGI1 in different S. enterica serovars, now including serovar Newport, strengthens the hypothesis of horizontal transfer of SGI1.

scholarly journals Molecular diversity and antibiotic resistance gene profile of Salmonella enterica serovars isolated from humans and food animals in Lagos, Nigeria

2019 ◽  
Vol 66 (4) ◽  
pp. 509-527 ◽  
Author(s):  
Abraham Ajayi ◽  
Stella Ifeanyi Smith ◽  
Julien Coulibaly Kalpy ◽  
Ibidunni Oreoluwa Bode-Sojobi ◽  
Yao Kouamé René ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Molecular Diversity ◽  
Antibiotic Resistance Gene ◽  
Gene Profile ◽  
Food Animals

scholarly journals Molecular Analysis of Antibiotic Resistance Gene Clusters in Vibrio cholerae O139 and O1 SXT Constins

2001 ◽  
Vol 45 (11) ◽  
pp. 2991-3000 ◽  
Author(s):  
Bianca Hochhut ◽  
Yasmin Lotfi ◽  
Didier Mazel ◽  
Shah M. Faruque ◽  
Roger Woodgate ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Vibrio Cholerae ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Dna Sequences ◽  
Antibiotic Resistance Gene ◽  
Antibiotic Resistance Genes ◽  
Gene Clusters ◽  
Vibrio Cholerae O139 ◽  
El Tor

ABSTRACT Many recent Asian clinical Vibrio cholerae E1 Tor O1 and O139 isolates are resistant to the antibiotics sulfamethoxazole (Su), trimethoprim (Tm), chloramphenicol (Cm), and streptomycin (Sm). The corresponding resistance genes are located on large conjugative elements (SXT constins) that are integrated into prfC on the V. cholerae chromosome. We determined the DNA sequences of the antibiotic resistance genes in the SXT constin in MO10, an O139 isolate. In SXTMO10, these genes are clustered within a composite transposon-like structure found near the element's 5′ end. The genes conferring resistance to Cm (floR), Su (sulII), and Sm (strA and strB) correspond to previously described genes, whereas the gene conferring resistance to Tm, designated dfr18, is novel. In some other O139 isolates the antibiotic resistance gene cluster was found to be deleted from the SXT-related constin. The El Tor O1 SXT constin, SXTET, does not contain the same resistance genes as SXTMO10. In this constin, the Tm resistance determinant was located nearly 70 kbp away from the other resistance genes and found in a novel type of integron that constitutes a fourth class of resistance integrons. These studies indicate that there is considerable flux in the antibiotic resistance genes found in the SXT family of constins and point to a model for the evolution of these related mobile elements.

scholarly journals Detection of Salmonella spp. in Retail Raw Food Samples from Vietnam and Characterization of Their Antibiotic Resistance

2007 ◽  
Vol 73 (21) ◽  
pp. 6885-6890 ◽  
Author(s):  
Thi Thu Hao Van ◽  
George Moutafis ◽  
Taghrid Istivan ◽  
Linh Thuoc Tran ◽  
Peter J. Coloe
Keyword(s):  
Antibiotic Resistance ◽  
Nalidixic Acid ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Antimicrobial Agents ◽  
Genomic Island ◽  
Antibiotic Resistance Gene ◽  
Food Samples ◽  
Salmonella Spp

ABSTRACT A study was conducted to examine the levels of Salmonella spp. contamination in raw food samples, including chicken, beef, pork, and shellfish, from Vietnam and to determine their antibiotic resistance characteristics. A total of 180 samples were collected and examined for the presence of Salmonella spp., yielding 91 Salmonella isolates. Sixty-one percent of meat and 18% of shellfish samples were contaminated with Salmonella spp. Susceptibility of all isolates to a variety of antimicrobial agents was tested, and resistance to tetracycline, ampicillin/amoxicillin, nalidixic acid, sulfafurazole, and streptomycin was found in 40.7%, 22.0%, 18.7%, 16.5%, and 14.3% of the isolates, respectively. Resistance to enrofloxacin, trimethoprim, chloramphenicol, kanamycin, and gentamicin was also detected (8.8 to 2.2%). About half (50.5%) of the isolates were resistant to at least one antibiotic, and multiresistant Salmonella isolates, resistant to at least three different classes of antibiotics, were isolated from all food types. One isolate from chicken (serovar Albany) contained a variant of the Salmonella genomic island 1 antibiotic resistance gene cluster. The results show that antibiotic resistance in Salmonella spp. in raw food samples from Vietnam is significant.

scholarly journals Salmonella Genomic Island 1 Multidrug Resistance Gene Clusters in Salmonella enterica Serovar Agona Isolated in Belgium in 1992 to 2002

2004 ◽  
Vol 48 (7) ◽  
pp. 2510-2517 ◽  
Author(s):  
Benoît Doublet ◽  
Patrick Butaye ◽  
Hein Imberechts ◽  
David Boyd ◽  
Michael R. Mulvey ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Gene Clusters ◽  
Complex Class ◽  
Class 1 Integron ◽  
Transposase Gene ◽  
New Variant ◽  
Class 1

ABSTRACT Salmonella genomic island 1 (SGI1) harbors a multidrug resistance (MDR) gene cluster which is a complex class 1 integron. Variant SGI1 MDR gene clusters conferring different MDR profiles have also been identified in several Salmonella enterica serovars and classified as SGI1-A to -F. A retrospective study was undertaken to characterize MDR regions from serovar Agona strains harboring SGI1 isolated from poultry in Belgium between 1992 and 2002. A total of 171 serovar Agona strains, displaying resistance to at least one antibiotic, were studied for the presence of SGI1. SGI1 was detected in 94 serovar Agona strains. The most prevalent variant was SGI1-A (85%), which harbors within the SGI1 complex class 1 integron a common region (CR1) containing orf513, a putative transposase gene, adjacent to the dfrA10 trimethoprim resistance gene. A new variant SGI1 named SGI1-G was identified in two strains. It consisted of the pse-1 gene cassette, as in SGI1-B, but with additional insertion of the orf513/dfrA10 region structure. Seven strains displaying the typical SGI1 MDR profile (Ap Cm Ff Sm Sp Su Tc) showed genetic variation at the 3′ end of SGI1. These strains harbored the insertion of the CR1 containing orf513 as in SGI1-A, -D, and -G. However, downstream the right end of CR1, they presented different 7.4- to 8.5-kb deletions of the SGI1 3′ end that extended to the chromosomal genes yieE and yieF. These results suggest a possible role of CR1 in deletion formation, as has been reported for some insertion sequences. Pulsed-field gel electrophoresis analysis showed that all the serovar Agona SGI1-carrying strains belonged to a single clone. Thus, SGI1 is largely encountered in serovar Agona strains isolated from poultry in Belgium, the most prevalent variant being SGI1-A. SGI1 MDR region undergoes recombinational events resulting in a diversity of MDR gene clusters.

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