scholarly journals Rapid selection of quinolone resistance in Campylobacter jejuni but not in Escherichia coli in individually housed broilers

2003 ◽  
Vol 52 (4) ◽  
pp. 719-723 ◽  
Author(s):  
M. van Boven
Keyword(s):  
Escherichia Coli ◽  
Campylobacter Jejuni ◽  
Quinolone Resistance ◽  
Selection Of

In Vivo Sequential Selection of Escherichia coli with Topoisomerase- and Efflux-Mediated Misleading Quinolone Resistance Phenotypes

2012 ◽  
Vol 18 (1) ◽  
pp. 19-22
Author(s):  
Mounira Smati ◽  
Jean-Philippe Emond ◽  
Guillaume Arlet ◽  
Jacques Tankovic
Keyword(s):  
Escherichia Coli ◽  
Quinolone Resistance ◽  
Sequential Selection ◽  
Selection Of

scholarly journals Sparfloxacin Selects Gyrase Mutations in First-Step Mycoplasma hominis Mutants, whereas Ofloxacin Selects Topoisonmerase IV Mutations

1999 ◽  
Vol 43 (10) ◽  
pp. 2493-2496 ◽  
Author(s):  
George E. Kenny ◽  
Patrick A. Young ◽  
Frank D. Cartwright ◽  
Karen E. Sjöström ◽  
Wai M. Huang
Keyword(s):  
Escherichia Coli ◽  
Mycoplasma Hominis ◽  
Quinolone Resistance ◽  
Second Step ◽  
Primary Target ◽  
Step Selection ◽  
Selection Of

ABSTRACT The role of mutations in the genes for GyrA and ParC in quinolone resistance in Mycoplasma hominis was studied. Selection with sparfloxacin gave mutations at GyrA83 (Ser→Leu;Escherichia coli numbering) or GyrA87 (Glu→Lys), and mutants had increased levels of resistance to sparfloxacin (8- to 16-fold) but not to ofloxacin. Selection with ofloxacin gave changes at ParC80 (Ser→Ile) or ParC84 (Glu→Lys), and mutants were four- to eightfold more resistant to ofloxacin but not to sparfloxacin. Selection of second-step mutants from strains with ParC mutations with either quinolone yielded double mutants with additional mutations at GyrA83 (Ser→Trp or Ser→Leu) or GyrA87 (Glu→Lys). Second-step selection of GyrA mutants gave additional mutations at ParC80 (Ser→Ile) or ParC84 (Glu→Lys). Two-step mutants showed high levels of resistance to ofloxacin (MICs, 64 to 128 μg/ml) and moderate levels of resistance to sparfloxacin (MICs, 2 to 8 μg/ml). The primary target of ofloxacin in first-step mutants of Mycoplasma hominis was ParC, whereas that for sparfloxacin was GyrA.

scholarly journals In Vivo Selection of Fluoroquinolone-Resistant Escherichia coli Isolates Expressing Plasmid-Mediated Quinolone Resistance and Expanded-Spectrum β-Lactamase

2006 ◽  
Vol 50 (4) ◽  
pp. 1525-1527 ◽  
Author(s):  
Laurent Poirel ◽  
Johann D. D. Pitout ◽  
Lucy Calvo ◽  
Jose-Manuel Rodriguez-Martinez ◽  
Deirdre Church ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Quinolone Resistance ◽  
Amino Acid Substitutions ◽  
In Vivo Selection ◽  
Susceptible Isolate ◽  
Canadian Patient ◽  
Selection Of

ABSTRACT A ciprofloxacin-resistant Escherichia coli isolate, isolate 1B, was obtained from a urinary specimen of a Canadian patient treated with norfloxacin for infection due to a ciprofloxacin-susceptible isolate, isolate 1A. Both isolates harbored a plasmid-encoded sul1-type integron with qnrA1 and bla VEB-1 genes. Isolate 1B had amino acid substitutions in gyrase and topoisomerase.

The selection of optimum fermentation condition for improved expression of sumo-il11 in escherichia coli

2015 ◽  
Vol 37 (1se) ◽  
Author(s):  
Nguyen Thi Quy ◽  
Duong Thu Huong ◽  
Dang Thi Ngoc Ha ◽  
Le Thi Thu Hong ◽  
Do Thi Huyen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fermentation Condition ◽  
Selection Of

scholarly journals Selection and co-selection of antibiotic resistances among Escherichia coli by antibiotic use in primary care: An ecological analysis

PLoS ONE ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. e0218134 ◽  
Author(s):  
Koen B. Pouwels ◽  
Berit Muller-Pebody ◽  
Timo Smieszek ◽  
Susan Hopkins ◽  
Julie V. Robotham
Keyword(s):  
Escherichia Coli ◽  
Primary Care ◽  
Antibiotic Use ◽  
Ecological Analysis ◽  
Selection Of

scholarly journals Comparison of Common Enrichment Broths Used in Diagnostic Laboratories for Shiga Toxin—Producing Escherichia coli

2021 ◽  
Vol 9 (3) ◽  
pp. 503
Author(s):  
Michael Bording-Jorgensen ◽  
Hannah Tyrrell ◽  
Colin Lloyd ◽  
Linda Chui
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Real Time Pcr ◽  
Acute Gastroenteritis ◽  
Diagnostic Testing ◽  
First Line ◽  
Gram Negative ◽  
Culture Independent ◽  
Surveillance Programs ◽  
Selection Of

Acute gastroenteritis caused by Shiga toxin-producing Escherichia coli (STEC) affects more than 4 million individuals in Canada. Diagnostic laboratories are shifting towards culture-independent diagnostic testing; however, recovery of STEC remains an important aspect of surveillance programs. The objective of this study was to compare common broth media used for the enrichment of STEC. Clinical isolates including O157:H7 as well as non-O157 serotypes were cultured in tryptic soy (TSB), MacConkey (Mac), and Gram-negative (GN) broths and growth was compared using culture on sheep’s blood agar and real-time PCR (qPCR). In addition, a selection of the same isolates was spiked into negative stool and enriched in the same three broths, which were then evaluated using culture on CHROMagarTM STEC agar and qPCR. TSB was found to provide the optimal enrichment for growth of isolates with and without stool. The results from this study suggest that diagnostic laboratories may benefit from enriching STEC samples in TSB as a first line enrichment instead of GN or Mac.

scholarly journals Use of tellurite for the selection of verocytotoxigenic Escherichia coli O157

1993 ◽  
Vol 39 (2) ◽  
pp. 155-158 ◽  
Author(s):  
P. M. Zadik ◽  
P. A. Chapman ◽  
C. A. Siddons
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Selection Of
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