scholarly journals Pharmacodynamics of linezolid in a clinical isolate of Streptococcus pneumoniae genetically modified to express lux genes

2003 ◽  
Vol 52 (3) ◽  
pp. 511-513 ◽  
Author(s):  
H. M. Alloush
Keyword(s):  
Streptococcus Pneumoniae ◽  
Clinical Isolate ◽  
Genetically Modified ◽  
Lux Genes

scholarly journals Expression of lux Genes in a Clinical Isolate of Streptococcus pneumoniae: Using Bioluminescence To Monitor Gemifloxacin Activity

2002 ◽  
Vol 46 (2) ◽  
pp. 538-542 ◽  
Author(s):  
S. J. Beard ◽  
V. Salisbury ◽  
R. J. Lewis ◽  
J. A. Sharpe ◽  
A. P. MacGowan
Keyword(s):  
Growth Rate ◽  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Clinical Isolate ◽  
Optical Density ◽  
Exponential Growth ◽  
Light Output ◽  
Photorhabdus Luminescens ◽  
Gram Positive Bacteria ◽  
Lux Genes

ABSTRACT A clinical isolate of Streptococcus pneumoniae was transformed with a plasmid containing the lux operon of Photorhabdus luminescens that had been modified to function in gram-positive bacteria. Cells containing this plasmid produced light stably and constitutively, without compromising the growth rate. Light output was correlated with measurements of optical density and viable counts during exponential growth and provided a sensitive, real-time measure of the pharmacodynamics of the fluoroquinolone gemifloxacin.

scholarly journals Heterogeneous Macrolide Resistance and Gene Conversion in the Pneumococcus

2006 ◽  
Vol 50 (1) ◽  
pp. 359-361 ◽  
Author(s):  
Nicole Wolter ◽  
Anthony M. Smith ◽  
David J. Farrell ◽  
Keith P. Klugman
Keyword(s):  
Streptococcus Pneumoniae ◽  
Gene Conversion ◽  
Clinical Isolate ◽  
Resistant Strain ◽  
Macrolide Resistance ◽  
Fitness Cost ◽  
Rrna Genes ◽  
23S Rrna ◽  
Resistant Phenotype ◽  
23S Rrna Genes

ABSTRACT A macrolide-resistant clinical isolate of Streptococcus pneumoniae with 23S rRNA mutations showed a heterogeneous phenotype and genotype. The mutant 23S rRNA genes from this isolate transformed susceptible strain R6 to resistance. Culture of resistant strain R6 in the absence of antibiotic pressure showed gene conversion to occur between the four 23S rRNA alleles, resulting in reversion to susceptibility with the resistant phenotype showing a fitness cost. These data explain the disappearance on subculture of heterogeneous macrolide resistance in the pneumococcus.

scholarly journals Characterization of a Genetic Element Carrying the Macrolide Efflux Gene mef(A) in Streptococcus pneumoniae

2000 ◽  
Vol 44 (9) ◽  
pp. 2585-2587 ◽  
Author(s):  
Maria Santagati ◽  
Francesco Iannelli ◽  
Marco R. Oggioni ◽  
Stefania Stefani ◽  
Gianni Pozzi
Keyword(s):  
Streptococcus Pneumoniae ◽  
Resistance Gene ◽  
Clinical Isolate ◽  
Open Reading Frames ◽  
Genetic Element ◽  
Reading Frames

ABSTRACT The mef(A) gene from a clinical isolate ofStreptococcus pneumoniae exhibiting the M-type resistance to macrolides was found to be part of the 7,244-bp chromosomal element Tn1207.1, which contained 8 open reading frames.orf2 encodes a resolvase/invertase, and orf5 is a homolog of the macrolide-streptogramin B resistance genemsr(SA).

scholarly journals Insertion Sequence 1515 in the ply Gene of a Type 1 Clinical Isolate of Streptococcus pneumoniae Abolishes Pneumolysin Expression

2007 ◽  
Vol 45 (7) ◽  
pp. 2296-2297 ◽  
Author(s):  
F. Garnier ◽  
R. P. Janapatla ◽  
E. Charpentier ◽  
G. Masson ◽  
C. Grelaud ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Clinical Isolate ◽  
Insertion Sequence

scholarly journals Mutation in 23S rRNA Responsible for Resistance to 16-Membered Macrolides and Streptogramins in Streptococcus pneumoniae

2001 ◽  
Vol 45 (1) ◽  
pp. 319-323 ◽  
Author(s):  
Florence Depardieu ◽  
Patrice Courvalin
Keyword(s):  
Escherichia Coli ◽  
Streptococcus Pneumoniae ◽  
Clinical Isolate ◽  
23S Rrna ◽  
Resistance Phenotype ◽  
Domain V

ABSTRACT Streptococcus pneumoniae clinical isolate BM4455 was resistant to 16-membered macrolides and to streptogramins. This unusual resistance phenotype was due to an A2062C (Escherichia coli numbering) mutation in domain V of the four copies of 23S rRNA.

scholarly journals Antibacterial Activity of Leaf Extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. Against Streptococcus pneumoniae

2020 ◽  
Vol 7 (1) ◽  
pp. 1-9
Author(s):  
Nuke Annisa Nasution ◽  
I Made Artika ◽  
Dodi Safari
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pneumoniae ◽  
Clinical Isolate ◽  
Antibacterial Agent ◽  
Antibacterial Agents ◽  
Leaf Extracts ◽  
Extract Concentration ◽  
Global Concern ◽  
Muntingia Calabura

Antibacterial resistance in Streptococcus pneumoniae has been increasing and is one of ongoing global concern.  The need to find new antibacterial agents against Streptococcus pneumoniae is of paramount importance. Medicinal plants are prospective sources of antibacterial agents. The aims of the present study were to determine the activity of leaf extraxt of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. against Streptococcus pneumoniae.  Leaves of Anredera cordifolia (Ten.) Steenis were extracted using 96% ethanol, while the leaves of Muntingia calabura L were extracted using 100% methanol.  The leaf extracts of the two plants obtained were bioassayed for antibacterial activity against Streptococcus pneumoniae ATCC 49619 and a clinical isolate Streptococcus pneumoniae PU 067.  Results showed that leaf extracts of both Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. have antibacterial activity in vitro against Streptococcus pneumoniae ATCC 49619 at crude extract concentrations of 25%, 50%, 75% and 100% (w/v). Both plants extracts showed strongest activity against S. pneumoniae ATCC 49619 at extract concentration of 75%.   In addition, the extracts of both plants have inhibitory activity against growth of the clinical isolate Streptococcus pneumoniae PU 067. Both plant extracts showed strongest activity against S. pneumoniae PU 067 at extract concentration of 100%.  Therefore, leaf extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. can potentially be used as a source of antibacterial agent for Streptococcus pneumoniae. Keywords: Antibacterial agent, Anredera cordifolia (Ten.) Steenis, Muntingia calabura L., Streptococcus pneumoniae.

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