scholarly journals Exosomes derived from human umbilical cord mesenchymal stem cells accelerate growth of VK2 vaginal epithelial cells through MicroRNAsin vitro

2018 ◽  
Vol 34 (2) ◽  
pp. 248-260 ◽  
Author(s):  
Zhongyi Zhu ◽  
Yijing Zhang ◽  
Yiqun Zhang ◽  
Hongdao Zhang ◽  
Wei Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Epithelial Cells ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Vaginal Epithelial Cells ◽  
Accelerate Growth

scholarly journals Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Prostate-Like Epithelial Cells In Vivo

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e102657 ◽  
Author(s):  
Wang Li ◽  
Bo Ye ◽  
Xiao-Yan Cai ◽  
Jian-Hua Lin ◽  
Wei-Qiang Gao
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Epithelial Cells ◽  
Umbilical Cord ◽  
Human Umbilical Cord

scholarly journals Human Umbilical Cord-Derived Mesenchymal Stem Cells Promote Corneal Epithelial Repair In Vitro

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1254
Author(s):  
Santhosh Kacham ◽  
Tejal Sunil Bhure ◽  
Sindhuja D. Eswaramoorthy ◽  
Gaurav Naik ◽  
Subha Narayan Rath ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Epithelial Cells ◽  
Umbilical Cord ◽  
Corneal Transplantation ◽  
Human Umbilical Cord ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Epithelial Repair

Corneal injuries are among the leading causes of blindness and vision impairment. Trauma, infectious keratitis, thermal and chemical (acids and alkali burn) injuries may lead to irreversible corneal scarring, neovascularization, conjunctivalization, and limbal stem cell deficiency. Bilateral blindness constitutes 12% of total global blindness and corneal transplantation remains a stand-alone treatment modality for the majority of end-stage corneal diseases. However, global shortage of donor corneas, the potential risk of graft rejection, and severe side effects arising from long-term use of immunosuppressive medications, demands alternative therapeutic approaches. Umbilical cord-derived mesenchymal stem cells can be isolated in large numbers using a relatively less invasive procedure. However, their role in injury induced corneal repair is largely unexplored. Here, we isolated, cultured and characterized mesenchymal stem cells from human umbilical cord, and studied the expression of mesenchymal (CD73, CD90, CD105, and CD34), ocular surface and epithelial (PAX6, WNT7A, and CK-8/18) lineage markers through immunofluorescence. The cultured human limbal and corneal epithelial cells were used as controls. Scratch assay was used to study the corneal epithelial repair potential of umbilical cord-derived mesenchymal stem cells, in vitro. The in vitro cultured umbilical cord-derived mesenchymal stem cells were plastic adherent, showed trilineage differentiation and expressed: mesenchymal markers CD90, CD105, CD73; epithelial marker CK-8/18, and ocular lineage developmental markers PAX6 and WNT-7A. Our findings suggest that umbilical cord-derived mesenchymal stem cells promote repair of the injured corneal epithelium by stimulating the proliferation of corneal epithelial cells, in vitro. They may serve as a potential non-ocular source of stem cells for treating injury induced bilateral corneal diseases.

Human umbilical cord-mesenchymal stem cells: a promising strategy for corneal epithelial regeneration

2020 ◽  
Vol 15 (3) ◽  
pp. 1381-1397 ◽  
Author(s):  
Siti Maisura Azmi ◽  
Mohamed Salih ◽  
Samar Abdelrazeg ◽  
Fatin Fazrina Roslan ◽  
Rafeezul Mohamed ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Epithelial Cells ◽  
Umbilical Cord ◽  
Cellular Migration ◽  
Human Umbilical Cord ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Telomerase ◽  
Epithelial Growth

Aim: As a strategy to improve the outcome of ex vivo cultivated corneal epithelial transplantation, the role of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) is investigated in promoting corneal epithelial growth and functions. Materials & methods: Human telomerase-immortalized corneal epithelial cells were characterized and its functions evaluated by scratch migration assay, cellular senescence, HLA expression and spheres formation with hUC-MSC. Results: Expression of corneal epithelial markers was influenced by the duration and method of co-culture. Indirect co-culture improved cellular migration and delayed senescence when treated after 3 and 5 days. hUC-MSC downregulated expression of HLA Class I and II in IFN-γ-stimulated human telomerase-immortalized corneal epithelial cells. Conclusion: hUC-MSC promote corneal epithelial growth and functions after treatment with hUC-MSC.

Sign in / Sign up

Export Citation Format

Share Document