scholarly journals Cell-free DNA in human follicular fluid as a biomarker of embryo quality

2014 ◽  
Vol 29 (12) ◽  
pp. 2661-2669 ◽  
Author(s):  
E. Scalici ◽  
S. Traver ◽  
N. Molinari ◽  
T. Mullet ◽  
M. Monforte ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Embryo Quality ◽  
Human Follicular Fluid ◽  
Cell Free Dna ◽  
Free Dna

scholarly journals Cell-free DNA in Human Follicular Fluid as Biomarker for Intracytoplasmic Sperm Injection Procedure Outcome

2021 ◽  
Vol 9 (B) ◽  
pp. 1745-1750
Author(s):  
Maanee Azzam ◽  
Adeela Hamood ◽  
Hind Abdulkadim
Keyword(s):  
Pregnancy Outcome ◽  
Follicular Fluid ◽  
Fertilization Rate ◽  
P Value ◽  
Human Follicular Fluid ◽  
Cell Free Dna ◽  
Free Dna ◽  
Detection Approach ◽  
Iraqi Women ◽  
High Level

Background: Follicular fluid considered as an important microenvironment for oocyte development, cell free-DNA (cfDNA) fragments that are found in this fluid and are released from cell apoptosis and/or necrosis, aimed to quantified the level of cf-DNA, in the follicular fluid and to assess any relation between the level of cf-DNA in this fluid with women’s age, duration of infertility, cause of infertility, her ovarian reserve values. Methods: Eighty-nine women were prospectively included in this study FF cf-DNA which was determined by conventional real time PCR-syber green detection approach which quantified by ALU-specific primers. Results: cell-free DNA (cfDNA) level in Follicular fluid samples of Iraqi women level was; cfDNA (Mean±SD, 0.916±0.106 ng/μl). there was no significant relation between cfDNA and pregnancy outcome, but very low level and very high level cf DNA were related to negative pregnancy outcome, cfDNA was second most important predictive factor of pregnancy outcome after fertilization rate, but both not statistically significant p value was (0.622 and 0.241) respectively. Conclusion: current study notice that cfDNA in the follicular fluid may mainly reflect the cellular activity and the balance between programed apoptosis and cell necrosis.

Cell-free DNA in human follicular fluid as new non-invasive biomarker of embyo quality

2014 ◽  
Vol 102 (3) ◽  
pp. e62
Author(s):  
E. Scalici ◽  
S. Traver ◽  
T. Mullet ◽  
A. Ferrières ◽  
M. Monforte ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Human Follicular Fluid ◽  
Cell Free Dna ◽  
Non Invasive ◽  
Free Dna

Cell-free DNA: a non-invasive test for assessing embryo quality

Placenta ◽  
2011 ◽  
Vol 32 ◽  
pp. S281-S282 ◽  
Author(s):  
Paola Scaruffi ◽  
Shanti Levi ◽  
Gian Paolo Tonini ◽  
Paola Anserini
Keyword(s):  
Embryo Quality ◽  
Cell Free Dna ◽  
Non Invasive ◽  
Invasive Test ◽  
Free Dna

scholarly journals Trimethylamine-N-oxide is present in human follicular fluid and is a negative predictor of embryo quality

2020 ◽  
Vol 35 (1) ◽  
pp. 81-88 ◽  
Author(s):  
R A Nagy ◽  
I Homminga ◽  
C Jia ◽  
F Liu ◽  
J L C Anderson ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Embryo Quality ◽  
Conflicts Of Interest ◽  
Medical Center ◽  
Academic Medical Center ◽  
Gut Bacteria ◽  
Human Follicular Fluid ◽  
Cross Sectional ◽  
Oocyte Fertilization ◽  
Negative Predictor

Abstract STUDY QUESTION Are levels of trimethylamine-N-oxide (TMAO) in human follicular fluid (FF) related to IVF outcomes? SUMMARY ANSWER Higher levels of TMAO are a negative predictor of oocyte fertilization and embryo quality. WHAT IS KNOWN ALREADY TMAO is a metabolic product of dietary choline and l-carnitine produced via subsequent enzymatic modifications by the intestinal microbiota and hepatocytes. TMAO promotes inflammatory and oxidative stress pathways and has been characterized as a causative biomarker for the development of cardiometabolic disease. STUDY DESIGN, SIZE, DURATION For the present cross-sectional study, samples (FF and plasma) from 431 modified natural cycle (MNC)-IVF cycles of 132 patients were collected prospectively between October 2014 and March 2018 in a single academic medical center. PARTICIPANTS/MATERIALS, SETTING, METHODS TMAO and its precursors (choline, l-carnitine and gamma-butyrobetaine) were measured by ultra-high-performance liquid chromatography/mass spectrometry in (i) matched FF and plasma from 63 MNC-IVF cycles, in order to compare metabolite levels in the two matrices and (ii) FF from 232 MNC-IVF cycles in which only one oocyte was retrieved at follicular puncture. The association between metabolite levels and oocyte fertilization, embryo fragmentation percentage, embryo quality and the occurrence of pregnancy was analyzed using multilevel generalized estimating equations with adjustment for patient and cycle characteristics. MAIN RESULTS AND THE ROLE OF CHANCE The level of choline was higher in FF as compared to matched plasma (P < 0.001). Conversely, the levels of TMAO and gamma-butyrobetaine were lower in FF as compared to plasma (P = 0.001 and P = 0.075, respectively). For all metabolites, there was a positive correlation between FF and plasma levels. Finally, levels of TMAO and its gut-derived precursor gamma-butyrobetaine were lower in FF from oocytes that underwent normal fertilization (TMAO: odds ratio [OR] 0.66 [0.49–0.90], P = 0.008 per 1.0-μmol/L increase; gamma-butyrobetaine: OR 0.77 [0.60–1.00], P = 0.047 per 0.1-μmol/L increase) and developed into top-quality embryos (TMAO: OR 0.56 [0.42–0.76], P < 0.001 per 1.0-μmol/L increase; gamma-butyrobetaine: OR 0.79 [0.62–1.00], P = 0.050 per 0.1-μmol/L increase) than in FF from oocytes of suboptimal development. LIMITATIONS, REASONS FOR CAUTION The individual contributions of diet, gut bacteria and liver to the metabolite pools have not been quantified in this analysis. WIDER IMPLICATIONS OF THE FINDINGS More research on the contribution of diet and the effect of gut bacteria on FF TMAO is warranted. Since TMAO integrates diet, microbiota and genetic setup of the person, our results indicate potential important clinical implications for its use as biomarker for lifestyle interventions to improve fertility. STUDY FUNDING/COMPETING INTEREST(S) No external funding was received for this project. The Department of Obstetrics and Gynecology of the University Medical Center Groningen received an unrestricted educational grant of Ferring Pharmaceutical BV, the Netherlands. The authors have no other conflicts of interest. TRIAL REGISTRATION NUMBER Netherlands Trial Register number NTR4409.

scholarly journals Cell‐free DNA content in follicular fluid: A marker for the developmental ability of porcine oocytes

2019 ◽  
Vol 19 (1) ◽  
pp. 95-103 ◽  
Author(s):  
Kana Ichikawa ◽  
Hidenori Shibahara ◽  
Komei Shirasuna ◽  
Takehito Kuwayama ◽  
Hisataka Iwata
Keyword(s):  
Follicular Fluid ◽  
Dna Content ◽  
Cell Free Dna ◽  
Free Dna

scholarly journals Follicular Fluid Oocyte/Cumulus-Free DNA Concentrations as a Potential Biomolecular Marker of Embryo Quality and IVF Outcome

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
M. Dimopoulou ◽  
G. Anifandis ◽  
C. I. Messini ◽  
K. Dafopoulos ◽  
S. Kouris ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Embryo Quality ◽  
Ivf Outcome ◽  
Quantitative Real Time Pcr ◽  
Direct Role ◽  
Free Dna ◽  
Detection Approach ◽  
Sybr Green Detection ◽  
Group 2 ◽  
Group 1

The present prospective study examined the follicular fluid oocyte/cumulus-free DNA concentrations (ff o/c-free DNA) during ovarian stimulation and the possible association between ff o/c-free DNA and embryological results such as embryo quality and pregnancy rate. Eighty-three women undergoing IV/ICSI-ET treatments were prospectively included in this study. ff o/c-free DNA was determined by conventional quantitative real time PCR-Sybr green detection approach. The 83 ff samples were categorized in two groups: group 1n=62with cumulus oocytes complexes (CoCs)≥2 and group 2n=21with CoCs = 1. Group 1 revealed significant higher embryo quality in terms of mean score of embryo transfer (MSET), but lower ff o/c-free DNA concentrations compared to group 2. The two groups showed comparable pregnancy rates (positive hCG and clinical pregnancy). The higher the ff o/c-free DNA concentration, the lower the number of produced oocytes. ff o/c-free DNA did not seem to have any direct role in the IVF outcome. Further research is required to clarify whether ff o/c-free DNA is a biomolecular marker of embryo quality and IVF outcome.

scholarly journals The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality

2015 ◽  
Vol 30 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Sara D.M. Valckx ◽  
Jessie De Bie ◽  
Ellen D. Michiels ◽  
Ilse G. Goovaerts ◽  
Usha Punjabi ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Embryo Quality ◽  
Developmental Competence ◽  
Bovine Oocyte ◽  
Human Follicular Fluid ◽  
Oocyte Developmental Competence
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