scholarly journals Discovery of a novel imprinted gene by transcriptional analysis of parthenogenetic embryonic stem cells

2010 ◽  
Vol 25 (8) ◽  
pp. 1927-1941 ◽  
Author(s):  
Hathaitip Sritanaudomchai ◽  
Hong Ma ◽  
Lisa Clepper ◽  
Sumita Gokhale ◽  
Randy Bogan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Transcriptional Analysis ◽  
Imprinted Gene

Comparative Transcriptional Analysis of Embryoid Body Versus Two-Dimensional Differentiation of Murine Embryonic Stem Cells

2008 ◽  
Vol 14 (10) ◽  
pp. 1603-1614 ◽  
Author(s):  
Darío E. Sepúlveda ◽  
Barbara A. Andrews ◽  
Juan A. Asenjo ◽  
Eleftherios Terry Papoutsakis
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Transcriptional Analysis ◽  
Two Dimensional ◽  
Murine Embryonic Stem Cells

scholarly journals Transcriptional analysis of early lineage commitment in human embryonic stem cells

2007 ◽  
Vol 7 (1) ◽  
pp. 12 ◽  
Author(s):  
Andrew L Laslett ◽  
Sean Grimmond ◽  
Brooke Gardiner ◽  
Lincon Stamp ◽  
Adelia Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Transcriptional Analysis ◽  
Lineage Commitment

scholarly journals Direct reprogramming of human embryonic to trophoblast stem cells

2021 ◽  
Author(s):  
Norah M.E. Fogarty ◽  
Ahmed Abdelbaki ◽  
Afshan McCarthy ◽  
Liani Devito ◽  
Alice E. Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Transcriptional Analysis ◽  
Differentiation Potential ◽  
Trophoblast Stem Cells ◽  
Cellular Models ◽  
Trophoblast Stem

AbstractDuring the first week of development, human embryos form a blastocyst comprised of an inner cell mass and trophectoderm (TE) cells, the latter of which are progenitors of placental trophoblast. Here we investigated the expression of transcripts in the human TE from early to late blastocyst stages. We identified enrichment of transcription factors GATA2, GATA3, TFAP2C and KLF5 and characterised their protein expression dynamics across TE development. By inducible overexpression and mRNA transfection we determined that these factors, together with MYC, are sufficient to establish induced trophoblast stem cells (iTSCs) from human embryonic stem cells. These iTSCs self-renew and recapitulate morphological characteristics, gene expression profiles and directed differentiation potential similar to existing human TSCs. Systematic omission of each or combinations of factors, revealed the critical importance of GATA2 and GATA3 for successful iTSC reprogramming. Altogether, these findings provide insights into the transcription factor network that may be operational in the human TE and broaden the methods for establishing cellular models of early human placental progenitor cells, which may be useful in the future to model placental-associated diseases.Summary statementTranscriptional analysis of human blastocysts reveals transcription factors sufficient to derive induced trophoblast stem cells from primed human embryonic stem cells.

Comparative Transcriptional Analysis of Embryoid Body Versus Two-Dimensional Differentiation of Murine Embryonic Stem Cells

2008 ◽  
Vol 0 (0) ◽  
pp. 080423075413219 ◽  
Author(s):  
Darío E. Sepúlveda ◽  
Barbara A. Andrews ◽  
Juan A. Asenjo ◽  
Eleftherios Terry Papoutsakis
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Transcriptional Analysis ◽  
Two Dimensional ◽  
Murine Embryonic Stem Cells

scholarly journals In Vitro Corticogenesis from Embryonic Stem Cells Recapitulates the In Vivo Epigenetic Control of Imprinted Gene Expression

2016 ◽  
pp. bhw102 ◽  
Author(s):  
Tristan Bouschet ◽  
Emeric Dubois ◽  
Christelle Reynès ◽  
Satya K. Kota ◽  
Stéphanie Rialle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Imprinted Gene ◽  
Epigenetic Control
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