scholarly journals Evaluation of male fertility potential by Toluidine Blue test for sperm chromatin structure assessment

2009 ◽  
Vol 24 (7) ◽  
pp. 1569-1574 ◽  
Author(s):  
I. Tsarev ◽  
M. Bungum ◽  
A. Giwercman ◽  
J. Erenpreisa ◽  
T. Ebessen ◽  
...  
Keyword(s):  
Toluidine Blue ◽  
Chromatin Structure ◽  
Male Fertility ◽  
Sperm Chromatin ◽  
Structure Assessment ◽  
Fertility Potential

Evaluation of fertility potential by toluidine blue test and the sperm chromatin structure assay

2007 ◽  
Vol 88 ◽  
pp. S301
Author(s):  
R. Mahfouz ◽  
A. Agarwal ◽  
T.M. Said ◽  
J. Erenpreiss ◽  
A. Giwercman ◽  
...  
Keyword(s):  
Toluidine Blue ◽  
Chromatin Structure ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Fertility Potential

Loss of livestock breeding efficiency due to uncompensable sperm nuclear defects

1999 ◽  
Vol 11 (1) ◽  
pp. 1 ◽  
Author(s):  
D. P. Evenson
Keyword(s):  
Chromatin Structure ◽  
Male Fertility ◽  
Nuclear Dna ◽  
Early Embryo ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Fertility Potential ◽  
Post Stress

An important goal of modern analyses of semen is to elucidate the molecular traits of mammalian sperm chromatin structural abnormalities, defined here as ‘uncompensable’, that lead to abnormalities in fertility, pronuclear formation, early embryo quality and pregnancy outcome. Sperm with uncompensable nuclear abnormalities are able to fertilize oocytes both in vivo and in vitro; however, due to the uncompensable trait(s), the embryo development may be abnormal. Uncompensable nuclear traits can be experimentally induced in bull sperm by a mild thermal insult to the testis. Sperm nuclear morphology abnormalities seen in ejaculates 11-days post stress are likely related to molecular changes in chromatin observed 3-days post stress by the flow cytometric sperm chromatin structure assay (SCSA). The SCSA measures the susceptibility of sperm nuclear DNA to denaturation in situ. This susceptibility has been correlated with the presence of DNA strand breaks that may be derived in part by oxidative stress and possibly by a unique, abortive apoptotic mechanism. The extent of DNA denaturation is not significantly related to the level of disulfide bonding between the chromatin protamines. The use of human sperm with uncompensable nuclear traits for artificial reproductive techniques is also discussed. The goal of this research is to remove from semen doses those sperm with uncompensable nuclear traits and thereby increase male fertility potential. Extra key words: male fertility potential, sperm chromatin structure assay (SCSA).

scholarly journals Toluidine blue cytometry test for sperm DNA conformation: comparison with the flow cytometric sperm chromatin structure and TUNEL assays

2004 ◽  
Vol 19 (10) ◽  
pp. 2277-2282 ◽  
Author(s):  
J. Erenpreiss ◽  
K. Jepson ◽  
A. Giwercman ◽  
I. Tsarev ◽  
Je. Erenpreisa ◽  
...  
Keyword(s):  
Toluidine Blue ◽  
Chromatin Structure ◽  
Dna Conformation ◽  
Sperm Chromatin ◽  
Flow Cytometric ◽  
Sperm Dna

scholarly journals Sperm chromatin structure and male fertility: biological and clinical aspects

2006 ◽  
Vol 8 (1) ◽  
pp. 11-29 ◽  
Author(s):  
J. Erenpreiss ◽  
M. Spano ◽  
J. Erenpreisa ◽  
M. Bungum ◽  
A. Giwercman
Keyword(s):  
Chromatin Structure ◽  
Male Fertility ◽  
Sperm Chromatin ◽  
Clinical Aspects

scholarly journals 262 SPERM CHROMATIN STRUCTURE, OXIDATIVE STRESS AND BASIC SEMEN PARAMETERS OF MEN FROM SUBFERTILE COUPLES

2005 ◽  
Vol 17 (2) ◽  
pp. 281
Author(s):  
M. Bochenek ◽  
P. Gogol ◽  
J. Janeczko
Keyword(s):  
Chromatin Structure ◽  
Sperm Concentration ◽  
World Health ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Progressive Motility ◽  
Fertility Potential ◽  
Health Organization ◽  
High Level

It is known that the mammalian sperm chromatin structure plays an important role in male fertility. In opposition to many other areas of biological research, the human sperm chromatin can be considered as a model for animal fertility investigations. This is due to the great number of males with high levels of chromatin abnormalities and the ease of tracking their fertility potential. The aim of the study was to find a relationship between sperm chromatin structure, level of reactive oxygen species (ROS) and the basic semen parameters: sperm concentration and motility. The semen from a total of 391 men from subfertile couples 22–51 years old was used. The sperm chromatin abnormalities were examined flow cytometrically according to the SCSA method (sperm chromatin structure assay; Evenson D.P. Methods In Cell Biology, vol. 33, 1990) and ROS level was examined by luminometry (Kolletis et al. 1999 Fertil. Steril.). Sperm concentration and motility were checked microscopically. Sperm concentration of the examined ejaculates ranged from 0.05 × 106/mL to 627.5 × 106/mL and progressive motility ranged from 0% to 70%. More than 30% of spermatozoa with abnormal chromatin (level considered as the infertility threshold) was found in 70 (17.9%) patients; 15–30% of spermatozoa with abnormal chromatin (level of decreased fertility potential) was found in 154 (39.4%) patients; and in 167 (42.7%) patients the number of abnormal spermatozoa did not exceed 15% (level of normal fertility potential; Evenson et al. 1999 Hum. Reprod.; Zini et al. 2001 Fertil. Steril.). High significant correlations were found between chromatin abnormality and: patients' age (0.1008, P = 0.017), sperm concentration (−0.2735, P < 0.001), progressive motility (−0.4365, P < 0.001), and ROS level (0.2709, P < 0.001). However in patients with normal sperm concentration (>20 × 106/mL, according to the World Health Organization), as many as 11.5% had a high level of chromatin abnormality (>30% of abnormal chromatin) and 29.7% of moderate chromatin abnormality (15–30% abnormal chromatin). Similarly, in patients with normal progressive sperm motility (>50%, according to the World Health Organization) 1.7% had a high level of chromatin abnormality (>30% of abnormal chromatin), and 33.9% had a moderate level of chromatin abnormality (15–30% abnormal chromatin). Contrary to the findings of many earlier investigations, a strong relationship between sperm chromatin damages and basic semen parameters was observed in this work. The sperm chromatin structure assay should be included in standard semen examination to avoid expensive and time consuming in vitro procedures for spermatozoa with damaged DNA.

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