scholarly journals 262 SPERM CHROMATIN STRUCTURE, OXIDATIVE STRESS AND BASIC SEMEN PARAMETERS OF MEN FROM SUBFERTILE COUPLES

2005 ◽  
Vol 17 (2) ◽  
pp. 281
Author(s):  
M. Bochenek ◽  
P. Gogol ◽  
J. Janeczko
Keyword(s):  
Chromatin Structure ◽  
Sperm Concentration ◽  
World Health ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Progressive Motility ◽  
Fertility Potential ◽  
Health Organization ◽  
High Level

It is known that the mammalian sperm chromatin structure plays an important role in male fertility. In opposition to many other areas of biological research, the human sperm chromatin can be considered as a model for animal fertility investigations. This is due to the great number of males with high levels of chromatin abnormalities and the ease of tracking their fertility potential. The aim of the study was to find a relationship between sperm chromatin structure, level of reactive oxygen species (ROS) and the basic semen parameters: sperm concentration and motility. The semen from a total of 391 men from subfertile couples 22–51 years old was used. The sperm chromatin abnormalities were examined flow cytometrically according to the SCSA method (sperm chromatin structure assay; Evenson D.P. Methods In Cell Biology, vol. 33, 1990) and ROS level was examined by luminometry (Kolletis et al. 1999 Fertil. Steril.). Sperm concentration and motility were checked microscopically. Sperm concentration of the examined ejaculates ranged from 0.05 × 106/mL to 627.5 × 106/mL and progressive motility ranged from 0% to 70%. More than 30% of spermatozoa with abnormal chromatin (level considered as the infertility threshold) was found in 70 (17.9%) patients; 15–30% of spermatozoa with abnormal chromatin (level of decreased fertility potential) was found in 154 (39.4%) patients; and in 167 (42.7%) patients the number of abnormal spermatozoa did not exceed 15% (level of normal fertility potential; Evenson et al. 1999 Hum. Reprod.; Zini et al. 2001 Fertil. Steril.). High significant correlations were found between chromatin abnormality and: patients' age (0.1008, P = 0.017), sperm concentration (−0.2735, P < 0.001), progressive motility (−0.4365, P < 0.001), and ROS level (0.2709, P < 0.001). However in patients with normal sperm concentration (>20 × 106/mL, according to the World Health Organization), as many as 11.5% had a high level of chromatin abnormality (>30% of abnormal chromatin) and 29.7% of moderate chromatin abnormality (15–30% abnormal chromatin). Similarly, in patients with normal progressive sperm motility (>50%, according to the World Health Organization) 1.7% had a high level of chromatin abnormality (>30% of abnormal chromatin), and 33.9% had a moderate level of chromatin abnormality (15–30% abnormal chromatin). Contrary to the findings of many earlier investigations, a strong relationship between sperm chromatin damages and basic semen parameters was observed in this work. The sperm chromatin structure assay should be included in standard semen examination to avoid expensive and time consuming in vitro procedures for spermatozoa with damaged DNA.

Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay

Zygote ◽  
2003 ◽  
Vol 11 (4) ◽  
pp. 367-371 ◽  
Author(s):  
Isabelle Oger ◽  
Christelle Da Cruz ◽  
Gilles Panteix ◽  
Yves Menezo
Keyword(s):  
Dna Fragmentation ◽  
Chromatin Structure ◽  
Inverse Relationship ◽  
Sperm Concentration ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Oxidative Products ◽  
Dna Fragmentation Index

In our work, we have used 8-hydroxy-deoxyguanosine (8-OH-dG), one of the major oxidative products of sperm DNA, in a population of patients consulting for infertility. We found an inverse relationship between sperm concentration and the log of the ratio of 8-OH-dG to dG (P<0.01). On the same patients' sperm samples, the sperm chromatin structure assay (SCSA) was performed. An inverse relationship was observed between the DNA fragmentation index and sperm concentration (P<0.001). There was also a positive relationship between SCSA and log 8-OH-dG/dG. This indicates that DNA fragmentation measured by the SCSA originates in part from oxidative products. In a few patients, antioxidant treatment decreased the DNA fragmentation index below the threshold of 30% that is crucial for subfertility.

Evaluation of fertility potential by toluidine blue test and the sperm chromatin structure assay

2007 ◽  
Vol 88 ◽  
pp. S301
Author(s):  
R. Mahfouz ◽  
A. Agarwal ◽  
T.M. Said ◽  
J. Erenpreiss ◽  
A. Giwercman ◽  
...  
Keyword(s):  
Toluidine Blue ◽  
Chromatin Structure ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Fertility Potential

scholarly journals Sperm chromatin structure assay and classical semen parameters: systematic review

2010 ◽  
Vol 20 (1) ◽  
pp. 114-124 ◽  
Author(s):  
J.A. Castilla ◽  
S. Zamora ◽  
M.C. Gonzalvo ◽  
J.D. Luna del Castillo ◽  
J.A. Roldan-Nofuentes ◽  
...  
Keyword(s):  
Systematic Review ◽  
Chromatin Structure ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay

Loss of livestock breeding efficiency due to uncompensable sperm nuclear defects

1999 ◽  
Vol 11 (1) ◽  
pp. 1 ◽  
Author(s):  
D. P. Evenson
Keyword(s):  
Chromatin Structure ◽  
Male Fertility ◽  
Nuclear Dna ◽  
Early Embryo ◽  
Sperm Chromatin ◽  
Sperm Chromatin Structure Assay ◽  
Fertility Potential ◽  
Post Stress

An important goal of modern analyses of semen is to elucidate the molecular traits of mammalian sperm chromatin structural abnormalities, defined here as ‘uncompensable’, that lead to abnormalities in fertility, pronuclear formation, early embryo quality and pregnancy outcome. Sperm with uncompensable nuclear abnormalities are able to fertilize oocytes both in vivo and in vitro; however, due to the uncompensable trait(s), the embryo development may be abnormal. Uncompensable nuclear traits can be experimentally induced in bull sperm by a mild thermal insult to the testis. Sperm nuclear morphology abnormalities seen in ejaculates 11-days post stress are likely related to molecular changes in chromatin observed 3-days post stress by the flow cytometric sperm chromatin structure assay (SCSA). The SCSA measures the susceptibility of sperm nuclear DNA to denaturation in situ. This susceptibility has been correlated with the presence of DNA strand breaks that may be derived in part by oxidative stress and possibly by a unique, abortive apoptotic mechanism. The extent of DNA denaturation is not significantly related to the level of disulfide bonding between the chromatin protamines. The use of human sperm with uncompensable nuclear traits for artificial reproductive techniques is also discussed. The goal of this research is to remove from semen doses those sperm with uncompensable nuclear traits and thereby increase male fertility potential. Extra key words: male fertility potential, sperm chromatin structure assay (SCSA).

scholarly journals Effect of Myoinositol and Antioxidants on Sperm Quality in Men with Metabolic Syndrome

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Mario Montanino Oliva ◽  
Elisa Minutolo ◽  
Assunta Lippa ◽  
Paola Iaconianni ◽  
Alberto Vaiarelli
Keyword(s):  
Metabolic Syndrome ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
World Health ◽  
Semen Parameters ◽  
Metabolic Profiles ◽  
Prospective Longitudinal Study ◽  
Semen Characteristics ◽  
Health Organization ◽  
Prospective Longitudinal

This prospective longitudinal study investigated the effects of a dietary supplement in patients affected by reduced sperm motility (asthenospermic males) with metabolic syndrome. The product tested was Andrositol®, which contains myoinositol (MI) as principal compound, in association with other molecules, and the parameters evaluated were semen characteristics as well as hormone and metabolic profiles. The inclusion criteria were subjects aged over 18 years, with asthenospermia and metabolic syndrome. The exclusion criteria were presence of cryptorchidism, varicocele, and prostatitis. For this study, 45 males who had such features were enrolled. Their selection was made according to the 2010 World Health Organization (WHO) criteria (5th Edition) for the Evaluation of Human Semen. Hormone and metabolic profiles and semen parameters were assessed at the beginning of the study and after three months of treatment with Andrositol. The differences between the values before and after the supplementation were found statistically significant. Andrositol normalized the metabolic profile of these patients, improving their insulin sensitivity. Moreover, testosterone levels were increased and the semen characteristics, such as sperm concentration, motility, and morphology, highly improved. In conclusion, the association of MI with other molecules (micronutrients and vitamins) could be an effective therapy for metabolic disorders, as well as hormonal and spermatic changes responsible for male infertility.

scholarly journals Effects of Varicocelectomy on Abnormal Semen Parameters in Patients with Clinically Palpable Varicocele

2018 ◽  
Vol 43 (2) ◽  
pp. 94-100
Author(s):  
Md. Selim Morshed ◽  
AKM Khurshidul Alam ◽  
AKM Anwarul Islam ◽  
Sojib Bin Zaman ◽  
Mohammad Saruar Alam ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Surgical Correction ◽  
World Health ◽  
Semen Parameters ◽  
Semen Parameter ◽  
Quasi Experimental ◽  
Health Organization ◽  
The Impact

Prevalence of varicocele is approximately 15.1% of the general population. Studies showed that surgical correction of clinically palpable varicocele could improve the semen parameters. However, there is scarce of study to demonstrate post-operative outcome of varicocelectomy among the patients with abnormal semen parameter. Therefore, this study tried to compare the microscopic changes in sperm concentration, sperm motility, and sperm morphology before and after varicocelectomy. This quasi-experimental study was done in Urology Department of Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh from November 2014 to April 2016. Forty five patients with clinically palpable varicocele were finally includedin the study. Semen parameters of these patients were assessed by following the World Health Organization′s 5th manual of semen parameter, 2010. Subinguinal varicocelectomy was performed in every case. Follow up was done three months and six months after surgery. Data were analysed with statistical software SPSS 20 and level of significance was assessed by paired t-test.Almost 60.0% of patients were from 25 to 35 years age group. Of them, 87.1% patients were presented with left-sided varicocele, and 80% were grade III. All the patients showed oligospermia on semen analysis, whereas the number of abnormal sperm motility and abnormal morphology was 39, and 09, respectively. After surgical correction of varicocele, mean improvement of sperm concentration, motility and morphology found in 35 patients (77.7%), 31 patients (79.4%) and six patients (66.6%) respectively. The improvement was statistically significant (p<0.05). This study showed that surgical treatment could lead to the betterment of semen parameters in patients with clinically palpable varicocele, which will guide in deciding the management of varicocele patients presented with abnormal semen parameter. However, to recommend varicocelectomy as a most useful procedure in treating infertility cases; further longitudinal studies are recommended to establish the impact of varicocelectomy on

Non-Communicable Diseases and Global Health Politics

2019 ◽  
pp. 626-659
Author(s):  
Roger Magnusson
Keyword(s):  
Respiratory Diseases ◽  
Communicable Diseases ◽  
World Health ◽  
Global Response ◽  
Non Communicable Diseases ◽  
Health Politics ◽  
Chronic Respiratory Diseases ◽  
Health Organization ◽  
High Level ◽  
And Control

Non-communicable diseases (NCDs), including cardiovascular disease, cancer, chronic respiratory diseases, and diabetes, are responsible for around 70 percent of global deaths each year. This chapter describes how NCDs have become prevalent and critically evaluates global efforts to address NCDs and their risk factors, with a particular focus on the World Health Organization (WHO) and United Nations (UN) system. It explores the factors that have prevented those addressing NCDs from achieving access to resources and a priority commensurate with their impact on people’s lives. The chapter evaluates the global response to NCDs both prior to and since the UN High-Level Meeting on Prevention and Control of Non-communicable Diseases, held in 2011, and considers opportunities for strengthening that response in future.

The effects of Pb on sperm parameters and sperm DNA fragmentation of men investigated for infertility

2020 ◽  
Vol 31 (4) ◽  
Author(s):  
G.U.S. Wijesekara ◽  
D.M.S. Fernando ◽  
S. Wijeratne
Keyword(s):  
Dna Fragmentation ◽  
Seminal Plasma ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
World Health ◽  
Sperm Dna Fragmentation ◽  
Progressive Motility ◽  
Sperm Dna ◽  
The Mean ◽  
Pb Concentration

AbstractBackgroundLead (Pb) is one of the metals most prevalent in the environment and is known to cause infertility and deoxyribonucleic acid (DNA) fragmentation. This study aimed to determine the association between seminal plasma Pb and sperm DNA fragmentation in men investigated for infertility.MethodsMale partners (n = 300) of couples investigated for infertility were recruited after informed consent was obtained. Sperm parameters were assessed according to the World Health Organization (WHO) guidelines. Seminal plasma Pb was estimated by atomic absorption spectrophotometry after digestion with nitric acid.ResultsIn Pb-positive and -negative groups the sperm parameters and sperm DNA fragmentation were compared using independent sample t-test and the Mann-Whitney U-test, respectively. The mean [standard deviation (SD)] age and duration of infertility were 34.8 (5.34) years and 45.7 (35.09) months, respectively, and the mean Pb concentration was 15.7 μg/dL. In Pb positives compared to Pb negatives the means (SD) of sperm count, progressive motility viability and normal morphology were lower (p > 0.05) but the DNA fragmentation was significantly higher 39.80% (25.08) than Pb negatives 22.65% (11.30). Seminal plasma Pb concentration and sperm DNA fragmentation had a positive correlation (r = 0.38, p = 0.03). A negative correlation was observed between sperm DNA fragmentation and sperm concentration, progressive motility, total motility and viability. When the DNA fragmentation was ≥30% sperm concentration and viability decreased (p < 0.05).ConclusionsPb in seminal plasma had a significant effect on sperm DNA fragmentation but not with other sperm parameters.

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