scholarly journals Preimplantation development of mouse oocytes activated by different levels of human phospholipase C zeta

2007 ◽  
Vol 23 (2) ◽  
pp. 365-373 ◽  
Author(s):  
Y. Yu ◽  
C.M. Saunders ◽  
F.A. Lai ◽  
K. Swann
Keyword(s):  
Phospholipase C ◽  
Preimplantation Development ◽  
Mouse Oocytes ◽  
Different Levels

scholarly journals Glycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xin-Yan Cao ◽  
Jack Rose ◽  
Shi-Yong Wang ◽  
Yong Liu ◽  
Meng Zhao ◽  
...  
Keyword(s):  
Germinal Vesicle ◽  
Preimplantation Development ◽  
Mouse Oocytes

scholarly journals Phospholipase C in mouse oocytes: characterization of β and γ isoforms and their possible involvement in sperm-induced Ca2+ spiking

1996 ◽  
Vol 316 (2) ◽  
pp. 583-591 ◽  
Author(s):  
Genevieve DUPONT ◽  
Orla M. McGUINNESS ◽  
Martin H. JOHNSON ◽  
Michael J. BERRIDGE ◽  
Franck BORGESE
Keyword(s):  
Monoclonal Antibodies ◽  
Phospholipase C ◽  
Kinase Inhibitors ◽  
Critical Role ◽  
Inhibitory Effect ◽  
Functional Studies ◽  
Mouse Oocytes ◽  
Mature Mouse

This study involved an investigation of the role of phospholipase C (PLC) in generating repetitive Ca2+ spikes at fertilization. Using a PCR-based strategy we have demonstrated that mouse oocytes have mRNA coding for PLCβ1, PLCβ3 and PLCγ isoenzymes. Furthermore, immunodetection of PLCγ1 using monoclonal antibodies reveals that PLCγ1 protein is present in mature mouse oocytes, ruling out the possibility that mRNA was being transcribed but not expressed. We were unsuccessful at detecting the presence of PLCβ protein, but the presence of this isoform can be inferred from functional studies. The PLC inhibitor, U73122, exerted an inhibitory effect on oocytes activated by spermatozoa or acetylcholine at concentrations of 10 and 30 μM respectively, while its inactive analogue had no effect. The soluble tyrosine kinase inhibitors, genistein (100 μM), herbimycin (10 μM) and geldanamycin (0.6 μM) which could affect signalling through PLCγ hindered but never completely inhibited Ca2+ spiking in response to fertilization. We conclude that the activation of PLC to generate InsP3 may play a critical role in fertilization.

Cryopreservation of ICR mouse oocytes: improved post-thawed preimplantation development after vitrification using Taxol™, a cytoskeleton stabilizer

2001 ◽  
Vol 75 (6) ◽  
pp. 1177-1184 ◽  
Author(s):  
Sung E Park ◽  
Hyung M Chung ◽  
Kwang Y Cha ◽  
Woo S Hwang ◽  
Eun S Lee ◽  
...  
Keyword(s):  
Preimplantation Development ◽  
Mouse Oocytes ◽  
Icr Mouse

scholarly journals Morphokinetic Analysis of Preimplantation Development of Mouse Oocytes Fertilised in Vitro Using a Non-humidifying Incubator With Time-lapse Cinematography (CCM-iBIS)

2021 ◽  
Author(s):  
Hiroyuki Watanabe ◽  
Haruka Ito ◽  
Ayumi Shintome ◽  
Hiroshi Suzuki
Keyword(s):  
Embryonic Development ◽  
Oxygen Tension ◽  
Developmental Rate ◽  
Time Lapse ◽  
Preimplantation Development ◽  
Mouse Oocytes ◽  
Lower Oxygen ◽  
Developmental Rates

Abstract Preimplantation development of mouse oocytes fertilised in vitro was assessed in a non-humidified incubator with time-lapse cinematography (CCM-iBIS). The developmental rates of embryos to the 4-cell and blastocyst stages under 5% CO2, 5% O2, and 90% N2 in CCM-iBIS were significantly higher than those under 5% CO2 in air in CCM-iBIS and a conventional CO2 incubator (CPO2-2301). The developmental speed of embryos was much faster in those cultured under lower oxygen tension in CCM-iBIS than in higher oxygen tension in CCM-iBIS and CPO2-2301. Embryonic development was much faster and more synchronised under lower oxygen tension. Non-humidified culture did not affect the development of the embryos. Mouse embryos cultured at lower oxygen tension reached 2-cell at 17 h, 3-cell at 39 h, 4-cell at 40 h, initiation of compaction at 58 h, morula at 70 h, and blastocyst at 82 h after insemination on average. Although compaction partially unravelled with cell division in compacting embryos, it appeared to complete depending on the timing of observation. Observation at a conventional 24-h interval 13 likely misjudges the developmental rate to morula. Determination of embryonic development 72 h after insemination may be more appropriate for “compacting morula” rather than “morula” in mice.

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