Preimplantation development following in vitro fertilization of mouse oocytes: Effects of timing of superovulation and preincubation in vitro

1987 ◽  
Vol 4 (2) ◽  
pp. 111-115 ◽  
Author(s):  
David H. Edgar ◽  
Katherine M. Whalley ◽  
John A. Mills
Keyword(s):  
In Vitro Fertilization ◽  
Preimplantation Development ◽  
Vitro Fertilization ◽  
Mouse Oocytes

scholarly journals A Brief Incubation of Cumulus-Enclosed Mouse Eggs in a Calcium-Free Medium Containing a High Concentration of Calcium-Chelator Markedly Improves Preimplantation Development

2020 ◽  
Vol 17 (10) ◽  
pp. 3505
Author(s):  
Valeria Merico ◽  
Silvia Garagna ◽  
Maurizio Zuccotti
Keyword(s):  
In Vitro Fertilization ◽  
Mammalian Species ◽  
Developmental Rate ◽  
Cumulus Cells ◽  
Fertilization Rate ◽  
Preimplantation Development ◽  
High Concentration ◽  
Vitro Fertilization ◽  
Positive Effects

The presence of cumulus cells (CCs) surrounding ovulated eggs is beneficial to in vitro fertilization and preimplantation development outcomes in several mammalian species. In the mouse, this contribution has a negligible effect on the fertilization rate; however, it is not yet clear whether it has positive effects on preimplantation development. Here, we compared the rates of in vitro fertilization and preimplantation development of ovulated B6C3F1 CC-enclosed vs. CC-free eggs, the latter obtained either after a 5 min treatment in M2 medium containing hyaluronidase or after 5–25 min in M2 medium supplemented with 34.2 mM EDTA (M2-EDTA). We found that, although the maintenance of CCs around ovulated eggs does not increment their developmental rate to blastocyst, the quality of the latter is significantly enhanced. Most importantly, for the first time, we describe a further quantitative and qualitative improvement, on preimplantation development, when CC-enclosed eggs are isolated from the oviducts in M2-EDTA and left in this medium for a total of 5 min prior to sperm insemination. Altogether, our results establish an important advancement in mouse IVF procedures that would be now interesting to test on other mammalian species.

scholarly journals Mouse oocytes derived from fetal germ cells are competent to support the development of embryos by in vitro fertilization

2008 ◽  
Vol 75 (11) ◽  
pp. 1688-1689
Author(s):  
Wei Shen ◽  
Lan Li ◽  
Donghui Zhang ◽  
Qingjie Pan ◽  
Mingxiao Ding ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Germ Cells ◽  
Vitro Fertilization ◽  
Mouse Oocytes

Effect of Heat Shock and Cysteamine During In Vitro Fertilization on Preimplantation Development of Bovine Embryo.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 258-258
Author(s):  
Miki Sakatani ◽  
Ahmed Zaky Balboula ◽  
Ken-ichi Yamanaka ◽  
Masashi Takahashi
Keyword(s):  
Heat Shock ◽  
In Vitro Fertilization ◽  
Preimplantation Development ◽  
Bovine Embryo ◽  
Vitro Fertilization

Development of vitrified mouse oocytes after in vitro fertilization

Cryobiology ◽  
1991 ◽  
Vol 28 (1) ◽  
pp. 50-54 ◽  
Author(s):  
T. Kono ◽  
O.Y. Kwon ◽  
T. Nakahara
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Mouse Oocytes

The effect of propofol on parthenogenetic activation, in vitro fertilization and early development of mouse oocytes

1997 ◽  
Vol 67 (4) ◽  
pp. 769-774 ◽  
Author(s):  
Cécile Janssenswillen ◽  
Frank Christiaens ◽  
Frédéric Camu ◽  
André Van Steirteghem
Keyword(s):  
In Vitro Fertilization ◽  
Early Development ◽  
Vitro Fertilization ◽  
Parthenogenetic Activation ◽  
Mouse Oocytes

Effects of In Vitro Aging of Mouse Oocytes on Metaphase II Spindle Morphology, In Vitro Fertilization and Subsequent Embryonic Development

2010 ◽  
Vol 27 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Kazumasa Takahashi ◽  
Hitomi Matsui ◽  
Ikue Takahashi ◽  
Hiromichi Matsumoto ◽  
Emiko Fukui ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryonic Development ◽  
Vitro Fertilization ◽  
Mouse Oocytes ◽  
In Vitro Aging

Enhancing in vitro fertilization of mouse oocytes by partial zona pellucida digestion

1992 ◽  
Vol 9 (2) ◽  
pp. 128-132 ◽  
Author(s):  
N. Mordel ◽  
S. Ohad ◽  
B. Zentner ◽  
J. G. Schenker ◽  
J. Gordon ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Zona Pellucida ◽  
Vitro Fertilization ◽  
Mouse Oocytes

scholarly journals Piezo-assisted In Vitro Fertilization of Mouse Oocytes with Spermatozoa Retrieved from Epididymides Stored at 4 Degree C

2008 ◽  
Vol 54 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Zhi-Qiang FAN ◽  
Xiu-Wei LI ◽  
Ying LIU ◽  
Qing-Gang MENG ◽  
Yan-Ping WANG ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Mouse Oocytes

scholarly journals In vitro fertilization of mouse oocytes

2020 ◽  
Author(s):  
ADITYA SANKAR ◽  
Javier Martin Gonzalez
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Mouse Oocytes

Abstract this protocol describes the procedure of in vitro fertilisation of mouse oocytes. The procedure is performed at the transgenic core facility by a specialist according to the protocols found here: https://www.infrafrontier.eu/knowledgebase/protocols/cryopreservation-protocols

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