Episodic air pollution is associated with increased DNA fragmentation in human sperm without other changes in semen quality

2007 ◽  
Vol 22 (12) ◽  
pp. 3263-3263 ◽  
Author(s):  
M. Jafarabadi
2005 ◽  
Vol 20 (10) ◽  
pp. 2776-2783 ◽  
Author(s):  
Jiri Rubes ◽  
Sherry G. Selevan ◽  
Donald P. Evenson ◽  
Dagmar Zudova ◽  
Miluse Vozdova ◽  
...  

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Juliana R. Pariz ◽  
Caroline Ranéa ◽  
Rosa A. C. Monteiro ◽  
Donald P. Evenson ◽  
Joël R. Drevet ◽  
...  

Cryopreservation processes can damage spermatozoa and impair structural and functional cell characteristics. Plasma, nuclear membranes, and cellular organelles can suffer from the freeze and thaw process. This study evaluates the protective and stimulant effect of melatonin and caffeine supplementation on the functional characteristics of human spermatozoa before and after freezing. Thirty seminal samples from normozoospermic men aged 19–45 years old collected between October 2012 and May 2017 were included. Semen samples were supplemented with either 2 mM melatonin (MEL) prior to cryopreservation, 2 mM caffeine (CAF) in postthaw, or CAF and MEL (CM) in precryopreservation and postthaw, respectively. Kinetics and seminal parameters, mitochondrial activity, DNA fragmentation, and reactive oxygen species (ROS) levels were analyzed before and after cryopreservation. A significant reduction in sperm concentration, total and progressive motility, sperm kinetics, and mitochondrial activity, as well as a significant increase in DNA fragmentation and ROS production in postthaw samples compared to fresh samples, was identified. After administration of a caffeine and/or melatonin supplement, there was a significant increase in progressive motility in the CAF (p=0.005) and CM (p=0.048) groups, as well as mitochondrial activity in the CM group (p<0.05). Cryopreservation has negative effects on overall sperm quality and increases ROS production. A combination of caffeine and melatonin in prefreeze and postthaw sperm samples has proven to be a very effective and simple way to improve semen quality. This will be particularly useful for initial low-quality semen samples, those which suffer the most from the freezing/thawing process.


2012 ◽  
Vol 97 (1) ◽  
pp. 39-45.e2 ◽  
Author(s):  
Conrado Avendaño ◽  
Ariela Mata ◽  
César A. Sanchez Sarmiento ◽  
Gustavo F. Doncel

2004 ◽  
Vol 82 (4) ◽  
pp. 871-877 ◽  
Author(s):  
T SAID ◽  
A AGARWAL ◽  
R SHARMA ◽  
E MASCHA ◽  
S SIKKA ◽  
...  

Vestnik ◽  
2021 ◽  
pp. 226-232
Author(s):  
К. К. Тлеуханов ◽  
Н. А. Алтыбаева ◽  
М. К. Отарбаев ◽  
Е. М. Тойшибеков ◽  
А. А. Тлеуханова

В статье представлены собранные данные о методах устранения повышенной частоты фрагментации ДНК у сперматозоидов, которые в некоторых исследованиях подтверждают, что введение антиоксидантов может снизить уровень фрагментации ДНК у сперматозоидов. The article presents collected data on methods of eliminating the increased frequency of DNA fragmentation in spermatozoa, which in some studies confirm that the introduction of antioxidants can reduce the level of DNA fragmentation in spermatozoa.


2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
J.E. Hart ◽  
I. Holland ◽  
S. Gillooly ◽  
G. Adamkiewicz ◽  
K.A. Anderson ◽  
...  

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