scholarly journals PHENOTYPIC DIVERSITY AMONG ALLELES AT THE PER-1 LOCUS OF NEUROSPORA CRASSA

Genetics ◽  
1976 ◽  
Vol 82 (4) ◽  
pp. 595-603 ◽  
Author(s):  
H Branch Howe ◽  
T E Johnson
Keyword(s):  
Neurospora Crassa ◽  
Phenotypic Diversity ◽  
Chromosomal Rearrangements ◽  
Phenotypic Differences ◽  
Genetic Complexity ◽  
Mutant Strains ◽  
Low Levels ◽  
The Individual

ABSTRACT Comparison of 11 perithecial color mutants suggested that all were alleles at the per-1 locus but nonetheless separable into two groups because of phenotypic differences. Three of the mutant strains produced orange perithecia and black ascospores, and eight produced paler, yellow perithecia and white ascospores. Perithecial phenotype was dependent upon the genotype of the protoperithecial parent; ascospore phenotype, upon the genotype of the individual ascospore. No evidence was found that the white ascospores were due to chromosomal rearrangements. No separation of the perithecial and ascospore phenotypes by recombination was observed in a cross between one of the mutants and a per-1  + strain. However, apparent low levels of recombination in crosses between some of the mutants indicated possible genetic complexity at the per-1 locus. The phase specificity of the per-1 mutations and the possible nature and mode of expression of the orange and yellow perithecial pigments are discussed.

scholarly journals Genomic diversity of Mycobacterium avium subsp. paratuberculosis: pangenomic approach for highlighting unique genomic features with newly constructed complete genomes

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Jaewon Lim ◽  
Hong-Tae Park ◽  
Seyoung Ko ◽  
Hyun-Eui Park ◽  
Gyumin Lee ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Phenotypic Diversity ◽  
Control Strategies ◽  
Genomic Diversity ◽  
Phenotypic Traits ◽  
Whole Genome ◽  
Genomic Features ◽  
Phenotypic Differences ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Type Strains

AbstractMycobacterium avium subsp. paratuberculosis (MAP) is a causative agent of Johne’s disease, which is a chronic granulomatous enteropathy in ruminants. Determining the genetic diversity of MAP is necessary to understand the epidemiology and biology of MAP, as well as establishing disease control strategies. In the present study, whole genome-based alignment and comparative analysis were performed using 40 publicly available MAP genomes, including newly sequenced Korean isolates. First, whole genome-based alignment was employed to identify new genomic structures in MAP genomes. Second, the genomic diversity of the MAP population was described by pangenome analysis. A phylogenetic tree based on the core genome and pangenome showed that the MAP was differentiated into two major types (C- and S-type), which was in keeping with the findings of previous studies. However, B-type strains were discriminated from C-type strains. Finally, functional analysis of the pangenome was performed using three virulence factor databases (i.e., PATRIC, VFDB, and Victors) to predict the phenotypic diversity of MAP in terms of pathogenicity. Based on the results of the pangenome analysis, we developed a real-time PCR technique to distinguish among S-, B- and C-type strains. In conclusion, the results of our study suggest that the phenotypic differences between MAP strains can be explained by their genetic polymorphisms. These results may help to elucidate the diversity of MAP, extending from genomic features to phenotypic traits.

scholarly journals Sexuality in Neurospora crassa I. Mutations to male sterility

1972 ◽  
Vol 19 (3) ◽  
pp. 191-204 ◽  
Author(s):  
J. Weijer ◽  
N. V. Vigfusson
Keyword(s):  
Neurospora Crassa ◽  
Male Sterility ◽  
Wide Spectrum ◽  
Female Parent ◽  
Behaviour Pattern ◽  
Type Locus ◽  
Development Cycle ◽  
Ultraviolet Light Irradiation ◽  
Mutant Strains ◽  
Aberrant Segregation

SUMMARYMutations giving rise to sexual sterility were induced in Neurospora crassa macroconidia by ultraviolet-light irradiation. Thirty mutants were isolated on the basis of their male sterility in crosses with a wild-type strain. When used as the male parent these mutants exhibited a wide spectrum of sexual behaviour patterns ranging from the production of only small brown protoperithecia (complete male sterility) to the production of large and normally pigmented perithecia but with an undeveloped ostiole and very few if any spores. For many of the mutants the behaviour pattern is different when the strain is used as the female parent. Segregation data reveal that none of these mutants represent mutations of the mating-type locus. These findings suggest that the sexual development cycle is blocked at various stages in the different mutant strains. All attempts to restore fertility by supplying various additives to the medium or by varying the incubation time and temperature were unsuccessful. Conidial viability tests carried out on many of the strains revealed no abnormality in this respect. The aberrant segregation patterns exhibited by many of the mutants are discussed.

scholarly journals STRESS RESISTANCE AND COPY STRATEGIES OF STUDENTS ’BEHAVIOR DURING THE EDUCATIONAL PROCESS IN HIGHER EDUCATION INSTITUTIONS

2021 ◽  
pp. 98-107
Author(s):  
Andrii Cherkashyn
Keyword(s):  
Higher Education ◽  
Stress Resistance ◽  
Mental Stress ◽  
Higher Education Institutions ◽  
Educational Process ◽  
Self Control ◽  
Psychological Science ◽  
Effective Activity ◽  
Low Levels ◽  
The Individual

The article considers the main approaches to the problem of stress resistance of students in higher education institutions of Ukraine. It is determined that a number of scientists consider stress resistance as a property of the individual that determines its effective activity, and others as cognitively conditioned mechanisms for overcoming stress. It is noted that in psychological science there are other views on the stress resistance of the individual, but when studying the stress resistance of students in the educational process of higher education, it is advisable to use the above approaches. Studies suggest that most students have low levels of psychological stress, mental stress and medium, high levels of resilience. The main coping strategies of students’ behavior in stressful situations are “confrontational coping”, “avoidance”, “self-control” and “problem solving planning”.

scholarly journals PENGARUH SKEMA KOMPENSASI, SELF EFFICACY DAN PENALARAN MORAL TERHADAP SLACK BUDGETING

2019 ◽  
Vol 4 (1) ◽  
pp. 77-96
Author(s):  
Nayang Helmayunita ◽  
Ade Elsa Betavia
Keyword(s):  
Moral Reasoning ◽  
Laboratory Experiment ◽  
Self Efficacy ◽  
Accounting Students ◽  
State University ◽  
Compensation Scheme ◽  
Budgetary Slack ◽  
Causal Factors ◽  
Low Levels ◽  
The Individual

This study aims to examine the effect of compensation schemes, self efficacy and moral reasoning on slack budgeting. In this study the hypothesis is proposed that in the slack inducing compensation scheme the slack budgeting will be greater than using the truth inducing compensation scheme, then managers with low self efficacy will do slack budgeting compared to those with high self efficacy. It is also proposed that the individual with low moral reasoning will do slack budgeting rather than the individual with high moral reasoning. The research design in this study was a quasi 2 x 2 laboratory experiment, with Accounting students who had sat in the 5th semester of Padang State University as lower level managers who participated in budgeting. The statistical method used to test the hypothesis is two-way ANOVA. This study provides results that the compensation scheme can affect slack budgeting actions, self efficacy has no effect on slack budgeting actions, and the interaction between slack inducing compensation schemes with low self efficacy has no effect on slack budgeting actions. Moral reasoning affects slack budgeting actions, and at lower level managers who have low levels of moral reasoning and low levels of self efficacy, interactions with slack inducing compensation schemes affect slack budgeting actions. The results of this study can contribute in the management accounting literature related to budgetary slack and its causal factors. Keyword: Slack Budgeting, Compensation Schemes, Self Efficacy, and Moral Reasoning.

scholarly journals Phenotypic diversity of bread wheat lines with introgressions from the diploid cereal Aegilops speltoides for technological properties of grain and f lour

2020 ◽  
Vol 24 (7) ◽  
pp. 738-746
Author(s):  
L. V. Shchukina ◽  
I. F. Lapochkina ◽  
T. A. Pshenichnikova
Keyword(s):  
Genetic Diversity ◽  
Bread Wheat ◽  
Phenotypic Diversity ◽  
Chromosomal Rearrangements ◽  
Diploid Species ◽  
Aegilops Speltoides ◽  
Technological Properties ◽  
Gluten Content ◽  
Spring Variety ◽  
Growing Conditions

The creation of varieties adapted to changing environmental conditions, resistant to various pathogens, and satisfying various grain purposes is impossible without using the genetic diversity of wheat. One of the ways to expand the genetic diversity of wheat is to introduce new variants of genes from the genetic pool of congeners and wild relatives into the genotypes of existing varieties. In this study, we used 10 lines from the Arsenal collection created on the genetic basis of the spring variety ‘Rodina’ and the diploid species Aegilops speltoides in the Federal Research Center “Nemchinovka” in 1994. The lines were previously characterized for the presence of translocations and chromosomal rearrangements cytologically and using molecular markers. Technological analyses were performed on grain obtained in Western Siberia and Moscow region. The aim of this study was to establish the possibilities of expanding the phenotypic diversity for technological properties of grain and flour as a result of such hybridization of bread wheat and the diploid cereal Aegilops speltoides. The variety ‘Rodina’ forms a vitreous grain with a high gluten content in Siberia, but has low physical properties of flour and dough. Five derived lines were found to have significantly higher protein and gluten content in grain. The highest values under both growing conditions were found in lines 73/00i, 82/00i, and 84/00i. Two lines (69/00i and 76/00i) showed a high flour strength and dough elasticity, characterizing the lines as strong and valuable in quality. These lines can be used for baking bread. Line 82/00i inherited from Ae. speltoides a soft-grain endosperm, which indicates the introgression of the Ha-Sp gene, homoeoallelic to the Ha gene of bread wheat, into ‘Rodina’. Flour of this line is suitable for the manufacture of confectionery without the use of technological additives. The lines generally retained their characteristics in different growing conditions. They can be attracted as donors of new alleles of genes that determine the technological properties of grain and resistance to biotic stresses.

scholarly journals LuxS-dependent AI-2 production is not involved in global regulation of natural product biosynthesis inPhotorhabdusandXenorhabdus

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3471 ◽  
Author(s):  
Antje K. Heinrich ◽  
Merle Hirschmann ◽  
Nick Neubacher ◽  
Helge B. Bode
Keyword(s):  
Regulatory System ◽  
Regulatory Rna ◽  
Insect Larvae ◽  
Phenotypic Differences ◽  
Global Regulators ◽  
Autoinducer 2 ◽  
Small Regulatory Rna ◽  
Mutant Strains ◽  
Different Strains

The Gram-negative bacteriaPhotorhabdusandXenorhabdusare known to produce a variety of different natural products (NP). These compounds play different roles since the bacteria live in symbiosis with nematodes and are pathogenic to insect larvae in the soil. Thus, a fine tuned regulatory system controlling NP biosynthesis is indispensable. Global regulators such as Hfq, Lrp, LeuO and HexA have been shown to influence NP production ofPhotorhabdusandXenorhabdus. Additionally, photopyrones as quorum sensing (QS) signals were demonstrated to be involved in the regulation of NP production inPhotorhabdus.In this study, we investigated the role of another possible QS signal, autoinducer-2 (AI-2), in regulation of NP production. The AI-2 synthase (LuxS) is widely distributed within the bacterial kingdom and has a dual role as a part of the activated methyl cycle pathway, as well as being responsible for AI-2 precursor production. We deletedluxSin three different entomopathogenic bacteria and compared NP levels in the mutant strains to the wild type (WT) but observed no difference to the WT strains. Furthermore, the absence of the small regulatory RNAmicA, which is encoded directly upstream ofluxS, did not influence NP levels. Phenotypic differences between theP. luminescens luxSdeletion mutant and an earlier describedluxSdeficient strain ofP. luminescenssuggested that two phenotypically different strains have evolved in different laboratories.

scholarly journals Adenosine kinase-deficient mutant of Neurospora crassa

1982 ◽  
Vol 152 (3) ◽  
pp. 1292-1294
Author(s):  
J M Magill ◽  
P Dalke ◽  
T S Lyda ◽  
C W Magill
Keyword(s):  
Neurospora Crassa ◽  
Resistant Mutant ◽  
Adenosine Kinase ◽  
Deficient Mutant ◽  
Wild Type ◽  
Purine Nucleotides ◽  
Mutant Strains

Tubercidin-resistant mutant strains of Neurospora crassa were isolated, and at least one appeared to be deficient in adenosine kinase. No significant differences in [8-14C]adenosine labeling of purine nucleotides or nucleosides were found between the wild type and the adenosine kinase-deficient strains.

Glutamate synthase levels in Neurospora crassa mutants altered with respect to nitrogen metabolism

1981 ◽  
Vol 1 (2) ◽  
pp. 158-164
Author(s):  
N S Dunn-Coleman ◽  
E A Robey ◽  
A B Tomsett ◽  
R H Garrett
Keyword(s):  
Neurospora Crassa ◽  
Glutamate Dehydrogenase ◽  
Nitrogen Metabolism ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Glutamate Synthase ◽  
Wild Type ◽  
Mutant Strains ◽  
Synthase Regulation ◽  
Glutamate Biosynthesis ◽  
Glutamine Limitation

Glutamate synthase catalyzes glutamate formation from 2-oxoglutarate plus glutamine and plays an essential role when glutamate biosynthesis by glutamate dehydrogenase is not possible. Glutamate synthase activity has been determined in a number of Neurospora crassa mutant strains with various defects in nitrogen metabolism. Of particular interest were two mutants phenotypically mute except in an am (biosynthetic nicotinamide adenine dinucleotide phosphate-glutamate dehydrogenase deficient, glutamate requiring) background. These mutants, i and en-am, are so-called enhancers of am; they have been redesignated herein as en(am)-1 and en(am)-2, respectively. Although glutamate synthase levels in en(am)-1 were essentially wild type, the en(am)-2 strain was devoid of glutamate synthase activity under all conditions examined, suggesting that en(am)-2 may be the structural locus for glutamate synthase. Regulation of glutamate synthase occurred to some extent, presumably in response to glutamate requirements. Glutamate starvation, as in am mutants, led to enhanced activity. In contrast, glutamine limitation, as in gln-1 mutants, depressed glutamate synthase levels.

Does Lead at Low Dose Affect Intelligence in Children?

PEDIATRICS ◽  
1981 ◽  
Vol 68 (6) ◽  
pp. 894-896
Author(s):  
Herbert L. Needleman ◽  
David Bellinger ◽  
Alan Leviton
Keyword(s):  
Adverse Effects ◽  
Low Dose ◽  
Intelligence Test ◽  
Pediatric Clinic ◽  
Study Find ◽  
Group Data ◽  
Low Levels ◽  
The One ◽  
The Individual

Ernhart et al, on the basis of their own follow-up data and an incorrect critique of a single study, find reason to question the entire literature documenting the adverse effects of low levels of lead. They assert that, if effects exist, they are minimal. To reach this sweeping conclusion, they contradict or ignore the findings of the earlier study by Perino and Ernhart, misread a table from the one study they single out for criticism, and draw debatable conclusions from their own data. We conclude by calling the readers' attention to this sentence: "While the effects of subclinical lead intoxication may not be noted in the individual cases seen in a pediatric clinic, analysis of group data indicate quite clearly (emphasis added) that performance on an intelligence test is impaired."

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