ON THE IN VITRO DEVELOPMENT OF PSEUDO-TRYPTOPHAN SYNTHASE ACTIVITY BY EXTRACTS OF NEUROSPORA CRASSA MUTANT STRAINS td6 AND td120

1966 ◽  
Vol 44 (5) ◽  
pp. 651-654 ◽  
Author(s):  
S. D. Wainwright ◽  
Sandra D. Halcrow ◽  
Loyola J. Metcalfe
Keyword(s):  
Neurospora Crassa ◽  
Tryptophan Synthase ◽  
In Vitro Development ◽  
Mutant Strains ◽  
Vitro Development

EVIDENCE OF A MICROSOMAL FACTOR REQUIRED FOR IN VITRO DEVELOPMENT OF PSEUDO-TRYPTOPHAN SYNTHASE ACTIVITY BY EXTRACTS OF NEUROSPORA CRASSA

1966 ◽  
Vol 44 (1) ◽  
pp. 77-83 ◽  
Author(s):  
S. D. Wainwright
Keyword(s):  
Neurospora Crassa ◽  
Type Strain ◽  
Wild Type Strain ◽  
Enzymic Activity ◽  
Tryptophan Synthase ◽  
Wild Type ◽  
In Vitro Development ◽  
Microsome Fraction ◽  
Vitro Development

Extracts of conidia of the td3 mutant strain of Neurospora crassa did not develop pseudo-tryptophan synthase enzyme activity under conditions leading to formation of the activity by equivalent extracts of the wild type strain. The defect in extracts of the td3 mutant appears to be located in the microsome fraction. The latter is unable to interact effectively with a component of the td3 mutant "post-microsome" fraction which will support in vitro development of the enzymic activity when supplemented with microsomes from other strains of N. crassa.

Quantification of the “In Vitro” Development of the Mouse Embryo Inner Ear

1973 ◽  
Vol 82 (1) ◽  
pp. 19-21 ◽  
Author(s):  
Thomas Van De Water ◽  
Robert J. Ruben
Keyword(s):  
Mouse Embryo ◽  
Scoring System ◽  
Organ Cultures ◽  
In Vitro Development ◽  
Mutant Strains ◽  
Sensory Structures ◽  
Vitro Analysis ◽  
Vitro Development ◽  
In Vitro Analysis

The development of sensory structures in organ cultures of the twelfth and thirteenth gestation day mouse otocysts has been quantified and the expected “in vitro” development may be predicted. The scoring system may be applied to the “in vitro” analysis of congenitally malformed ears in mutant strains of mice.

Quantification of the “In Vitro” Development of the Mouse Embryo Inner Ear

1973 ◽  
Vol 82 (4_suppl) ◽  
pp. 19-21 ◽  
Author(s):  
Thomas Van De Water
Keyword(s):  
Mouse Embryo ◽  
Scoring System ◽  
Organ Cultures ◽  
In Vitro Development ◽  
Mutant Strains ◽  
Sensory Structures ◽  
Vitro Analysis ◽  
Vitro Development ◽  
In Vitro Analysis

The development of sensory structures in organ cultures of the twelfth and thirteenth gestation day mouse otocysts has been quantified and the expected “in vitro” development may be predicted. The scoring system may be applied to the “in vitro” analysis of congenitally malformed ears in mutant strains of mice.

In vitro development of human triploid zygotes reconstructed by pronuclear transfer

2002 ◽  
Vol 78 ◽  
pp. S180-S181
Author(s):  
John Zhang ◽  
Yi Ming Shu ◽  
Lewis C Krey ◽  
Hui Liu ◽  
Guang Lun Zhuang ◽  
...  
Keyword(s):  
In Vitro Development ◽  
Pronuclear Transfer ◽  
Vitro Development

Gallic acid promotes the in vitro development of sheep secondary isolated follicles involving the phosphatidylinositol 3-kinase pathway

2021 ◽  
pp. 106767
Author(s):  
Gizele A.L. Silva ◽  
Luana B. Araújo ◽  
Larissa C.R. Silva ◽  
Bruna B. Gouveia ◽  
Ricássio S. Barberino ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Phosphatidylinositol 3 Kinase ◽  
In Vitro Development ◽  
Vitro Development ◽  
Kinase Pathway ◽  
Phosphatidylinositol 3

scholarly journals Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets

2021 ◽  
Vol 22 (16) ◽  
pp. 8367
Author(s):  
Hien Lau ◽  
Shiri Li ◽  
Nicole Corrales ◽  
Samuel Rodriguez ◽  
Mohammadreza Mohammadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oxygen Consumption ◽  
Insulin Secretion ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
In Vitro Development ◽  
Porcine Islets ◽  
Vitro Development

Pre-weaned porcine islets (PPIs) represent an unlimited source for islet transplantation but are functionally immature. We previously showed that necrostatin-1 (Nec-1) immediately after islet isolation enhanced the in vitro development of PPIs. Here, we examined the impact of Nec-1 on the in vivo function of PPIs after transplantation in diabetic mice. PPIs were isolated from pancreata of 8–15-day-old, pre-weaned pigs and cultured in media alone, or supplemented with Nec-1 (100 µM) on day 0 or on day 3 of culture (n = 5 for each group). On day 7, islet recovery, viability, oxygen consumption rate, insulin content, cellular composition, insulin secretion capacity, and transplant outcomes were evaluated. While islet viability and oxygen consumption rate remained high throughout 7-day tissue culture, Nec-1 supplementation on day 3 significantly improved islet recovery, insulin content, endocrine composition, GLUT2 expression, differentiation potential, proliferation capacity of endocrine cells, and insulin secretion. Adding Nec-1 on day 3 of tissue culture enhanced the islet recovery, proportion of delta cells, beta-cell differentiation and proliferation, and stimulation index. In vivo, this leads to shorter times to normoglycemia, better glycemic control, and higher circulating insulin. Our findings identify the novel time-dependent effects of Nec-1 supplementation on porcine islet quantity and quality prior to transplantation.

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