scholarly journals RECOMBINATION IN DROSOPHILA MELANOGASTER MALE

Genetics ◽  
1973 ◽  
Vol 73 (3) ◽  
pp. 439-444
Author(s):  
Yuichiro Hiraizumi ◽  
Barton Slatko ◽  
Charles Langley ◽  
Annegreth Nill
Keyword(s):  
Drosophila Melanogaster ◽  
High Frequency ◽  
Centromeric Region ◽  
Frequent Occurrence ◽  
Complementary Products ◽  
Male Recombination ◽  
Considerable Fraction ◽  
The Third

ABSTRACT T-007 strain of Drosophila melanogaster is known to show recombination in males. The present study established the following points: (1) Clustering occurrence of recombinant, unequal recovery of complementary products of recombination, relatively high frequency of recombination around centromeric region, and relatively frequent occurrence of mosaic phenontype flies—all of these seem to indicate that a considerable fraction of male recombination in the T-007 strain is of premeiotic, or somatic origin, although a fraction still could be of meiotic origin; (2) Male recombination occurs in the third as well as in the second chromosomes, and the frequencies of recombinations are comparable between these two chromosome pairs.

scholarly journals SELECTION FOR MALE RECOMBINATION IN DROSOPHILA MELANOGASTER

Genetics ◽  
1976 ◽  
Vol 84 (2) ◽  
pp. 333-351
Author(s):  
Margaret G Kidwell ◽  
J F Kidwell
Keyword(s):  
Drosophila Melanogaster ◽  
Centromeric Region ◽  
Major Effect ◽  
Specific Region ◽  
Correlated Responses ◽  
Male Recombination ◽  
The Third ◽  
High Line ◽  
Two Component ◽  
Selection For

ABSTRACT Two-way selection for male recombination over seven intervals of the third chromosome in Drosophila melanogaster was practiced for nine generations followed by relaxed selection for five generations. Significant responses in both directions were observed but these mainly occurred in early generations in the low line and in later generations in the high line. Divergence of male recombination frequencies between the two selection lines was not restricted to any specific region but occurred in every measured interval of the chromosome. However, right-arm intervals showed a more pronounced response than either left-arm intervals or the centromeric region. Correlated responses in sterility and distortion of transmission ratios occurred as a result of selection for male recombination. Cluster distributions of male recombinants suggested a mixture of meiotic and late gonial events but relative map distances more closely resembled those of the salivary chromosome than standard meiotic or mitotic distances. Patterns of male recombination over time in both second and third chromosomes strongly suggested a major effect associated with the presence of third chromosomes from the Harwich strain. Evidence was also found for modifiers with relatively small effects located in other regions of the genome. The overall results are interpreted in terms of a two-component model of hybrid dysgenesis.

scholarly journals INVESTIGATION OF THE NATURE OF P-INDUCED MALE RECOMBINATION IN DROSOPHILA MELANOGASTER

Genetics ◽  
1985 ◽  
Vol 110 (2) ◽  
pp. 257-279
Author(s):  
Donald A R Sinclair ◽  
Thomas A Grigliatti
Keyword(s):  
Drosophila Melanogaster ◽  
Distribution Pattern ◽  
High Frequency ◽  
Radiation Treatment ◽  
Chromosomal Distribution ◽  
Mutagenic Potential ◽  
Male Recombination ◽  
Pleiotropic Mutation

ABSTRACT The present study consists of an investigation of P-induced male recombination in Drosophila melanogaster from a number of perspectives. In an initial set of experiments, male recombination induced by several different P strains was examined on both major autosomes. The ability of these P strains to evoke recombination is striking; in many cases it exceeded that of radiation treatment. Also of interest is the apparent nonrandom chromosomal distribution of P-exchange breakpoints. The data suggest that both recombinagenic capacity and distribution pattern of exchange breakpoints may be P-strain specific. In addition to these findings, we have confirmed previous indications that P-induced exchange is reasonably symmetrical and that it frequently occurs during premeiotic stages of spermatogenesis. Moreover, we have established that radiation and P background act additively with regard to the induction of male recombination. The second part of the work involved an analysis of heterochromatic vs. euchromatic recombination induced by several recombinagenically potent P strains. Results of these experiments have confirmed our earlier findings concerning the recombinagenic capacity of P strains. More importantly, it would appear that P-induced exchange in heterochromatin is rare. The induction of various kinds of mutations was also monitored in several of these experiments. The results indicate that the mutagenic potential of the P strains is substantial and of particular interest, that certain types of mutations are P-strain specific. For example, rare heterochromatic lesions were recovered exclusively in the experiment using the h12 strain, whereas a novel pleiotropic mutation occurred at a high frequency only in the T-007 experiment. Our findings are discussed within the context of a model of P-induced exchange.

scholarly journals EVIDENCE FOR NEWLY INDUCED GENETIC ACTIVITY RESPONSIBLE FOR MALE RECOMBINATION INDUCTION IN DROSOPHILA MELANOGASTER

Genetics ◽  
1978 ◽  
Vol 90 (1) ◽  
pp. 105-124
Author(s):  
Barton E Slatko
Keyword(s):  
Drosophila Melanogaster ◽  
Major Element ◽  
Centromeric Region ◽  
Natural Populations ◽  
Recombination Activity ◽  
Male Recombination ◽  
High Frequencies ◽  
Genetic Activity

ABSTRACT The T-007 second chromosomal line of Drosophila melanogaster, previously shown to contain a major element, Mr, responsible for male recombination induction, also contains the genetic capability to induce male recombination activity into (nonhomologous) third chromosomes. This newly induced male recombination activity maps to the centromeric region of two third-chromosome lines that were subjected to mapping experiments. The ability of these third chromosome lines to induce male recombination accounts for previous observations concerning the ability of Mr  + genotypes (derived from Mr/Mr  + heterozygous females) to induce male recombination for only a few generations, when only second chromosomes were selected and backcrossed. The occurrence of this effect, and a similar effect induced in the homologue of T-007, suggests a possible explanation of how natural populations of D. melanogaster have come to contain such high frequencies of these "male recombination" second and third chromosomes, despite their numerous deleterious effects.

scholarly journals Structure, frequency and distribution of P elements in relation to P—M hybrid dysgenic male recombination in Drosophila melanogaster

1989 ◽  
Vol 53 (3) ◽  
pp. 163-171 ◽  
Author(s):  
K. A. Exley ◽  
P. Eggleston
Keyword(s):  
Drosophila Melanogaster ◽  
Egg Production ◽  
Polytene Chromosomes ◽  
Element Distribution ◽  
Hybrid Dysgenesis ◽  
P Element ◽  
Male Recombination ◽  
P Elements ◽  
Egg Hatchability ◽  
The Third

SummaryThe frequency and distribution of P elements were investigated in the third chromosomes of two wild-type strains of Drosophila melanogaster using in situ hybridization of biotinylated probes to the polytene chromosomes. The relationship between these data and the extent of hybrid dysgenesis was determined through assays of egg production, egg hatchability (F2 embryo lethality), snw destabilization and male recombination along the third chromosome. The results suggest that P-element distribution, frequency and structure are all contributory factors in the regulation of hybrid dysgenesis. Texas 6 was shown consistently to be a stronger P strain than Texas 1, eliciting greater reductions in fertility, more extensive snw destabilization and higher frequencies of male recombination. Clustering of male recombination events, arising from pre-meiotic crossing over, was evident among the dysgenic progeny of each strain. Male recombination and snw destabilization were independently distributed among the dysgenic males studied, suggesting that these traits represent separate P-mediated functions. The third chromosome male recombination maps produced by the two strains differed significantly from each other and from the published female meiotic and polytene chromosome maps. Male recombination breakpoints were associated with the original distribution of P sequences in the two strains and the results suggest that this relationship may be closer for potentially complete P factors than for P sequences in general. An analysis of sub-lines derived from individual recombinant males revealed that chromosomal breakpoints could also be associated with novel insertions following P-element transposition.

scholarly journals AN ANALYSIS OF MALE-RECOMBINATION ELEMENTS IN A NATURAL POPULATION OF DROSOPHILA MELANOGASTER IN SOUTH TEXAS

Genetics ◽  
1978 ◽  
Vol 88 (1) ◽  
pp. 81-91
Author(s):  
Kathleen A Matthews ◽  
Yuichiro Hiraizumi
Keyword(s):  
Drosophila Melanogaster ◽  
Natural Population ◽  
Sex Chromosomes ◽  
South Texas ◽  
Male Recombination ◽  
Transmission Frequency ◽  
The Third ◽  
Y Chromosomes

ABSTRACT Genomes from a group of Drosophila melanogmter collected from a natural population at San Benito, South Texas, in March of 1975 were analyzed for the presence of male-recombination elements. All three autosomes and both sex chromosomes were examined, with emphasis placed on the two major autosomes, the second and third chromosomes. In samples of 16 second and 16 third chromosomes, at least half, but not all, of each were found to carry male-recombination elements. It is suggested, although the data are not conclusive, that some of the fourth, X, and Y chromosomes might also be associated with male-recombination elements.—When a male-recombination element, or elements, was located in the second chromosome, relatively more male recombination was induced in the second than in the third chromosome. This situation was reversed when the element(s) was located in the third chromosome.—Distortion of transmission frequency, one of the characteristics of previously studied second chromosome lines associated with male recombination, was confirmed for these second chromosomes that carried male-recombination elements. Similar, but less pronounced, distortion was observed for the third chromosome lines that carried male-recombination elements.

scholarly journals An SD (Segregation Distribution) – MR (Male Recombination) chromosome isolated from a natural population of Drosophila melanogaster

1984 ◽  
Vol 43 (2) ◽  
pp. 149-158
Author(s):  
Gábor Bencze ◽  
Barton E. Slatko
Keyword(s):  
Drosophila Melanogaster ◽  
Natural Population ◽  
X Chromosome ◽  
Segregation Distortion ◽  
Reciprocal Cross ◽  
Centromeric Region ◽  
Meiotic Drive ◽  
Recombination Activity ◽  
Male Recombination ◽  
Lines Of Evidence

SUMMARYA second chromosome line of Drosophila melanogaster (S-90), isolated from a northern California natural population, is able to induce (1) an increased frequency of X-chromosome visible mutations, (2) male recombination activity subject to reciprocal cross suppression, and (3) strong meiotic drive from heterozygous males. Based upon several lines of evidence (including the response to suppressor chromosomes of both systems) we conclude that S-90 contains both SD (Segregation Distortion) and MR (P or I) chromosome activity. The two systems appear to behave independently and simultaneously, and a small centromeric region of the S-90 chromosome appears to contain the major genetic elements of both systems.

Аpplying of high-frequency monopolar diathermocoagulation in the treatment of chronic pulpitis

2020 ◽  
Vol 1 (12) ◽  
pp. 40-42
Author(s):  
F. Yu. Daurova ◽  
D. I. Tomaeva ◽  
S. V. Podkopaeva ◽  
Yu. A. Taptun
Keyword(s):  
Root Canal ◽  
High Frequency ◽  
Antibacterial Effect ◽  
Comparison Group ◽  
Poor Quality ◽  
Endodontic Treatment ◽  
Root Canals ◽  
The Third ◽  
Before And After

Relevance: the reason for the development of complications in endodontic treatment is poor-quality instrumental treatment root canals.Aims: a study of the animicrobial action and clinical efficacy of high-frequency monopolar diathermocoagulation in the treatment of chronic forms of pulpitis.Materials and methods: 102 patients with various chronic forms of pulpitis were divided into three groups of 34 patients each. In the first two groups, high-frequency monopolar diathermocoagulation was used in endodontic treatment in different modes. In the third group, endodontic treatment was carried out without the use of diathermocoagulation (comparison group). The root canal microflora in chronic pulpitis in vivo was studied twice-before and after diathermocoagulation.Results: it was established that high-frequency monopolar diathermocoagulation in the effect mode is 3, power is 4 (4.1 W) and effect is 4, power is 4 (5.4 W) with an exposure time of 3 seconds, it has a pronounced antibacterial effect on all presented pathogenic microflora obtained from the root canals of the teeth.

scholarly journals A Cluster of Cuticle Protein Genes of Drosophila melanogaster at 65A: Sequence, Structure and Evolution

Genetics ◽  
1997 ◽  
Vol 147 (3) ◽  
pp. 1213-1224
Author(s):  
Jean-Philippe Charles ◽  
Carol Chihara ◽  
Shamim Nejad ◽  
Lynn M Riddiford
Keyword(s):  
Drosophila Melanogaster ◽  
Genomic Dna ◽  
Multiple Copy ◽  
Sequence Structure ◽  
Coding Regions ◽  
The Third ◽  
Genetic Complexity ◽  
Genes Encoding ◽  
Cuticle Protein ◽  
Cuticle Proteins

A 36-kb genomic DNA segment of the Drosophila melanogaster genome containing 12 clustered cuticle genes has been mapped and partially sequenced. The cluster maps at 65A 5-6 on the left arm of the third chromosome, in agreement with the previously determined location of a putative cluster encompassing the genes for the third instar larval cuticle proteins LCP5, LCP6 and LCP8. This cluster is the largest cuticle gene cluster discovered to date and shows a number of surprising features that explain in part the genetic complexity of the LCP5, LCP6 and LCP8 loci. The genes encoding LCP5 and LCP8 are multiple copy genes and the presence of extensive similarity in their coding regions gives the first evidence for gene conversion in cuticle genes. In addition, five genes in the cluster are intronless. Four of these five have arisen by retroposition. The other genes in the cluster have a single intron located at an unusual location for insect cuticle genes.

scholarly journals GENETIC AND BIOCHEMICAL BASIS OF ENZYME ACTIVITY VARIATION IN NATURAL POPULATIONS. I. ALCOHOL DEHYDROGENASE IN DROSOPHILA MELANOGASTER

Genetics ◽  
1978 ◽  
Vol 89 (2) ◽  
pp. 371-388
Author(s):  
John F McDonald ◽  
Francisco J Ayala
Keyword(s):  
Gene Regulation ◽  
Drosophila Melanogaster ◽  
Alcohol Dehydrogenase ◽  
Natural Populations ◽  
Difficult Problem ◽  
Regulatory Genes ◽  
Biochemical Basis ◽  
Evolutionary Consequence ◽  
The Third ◽  
Adh Activity

ABSTRACT Recent studies by various authors suggest that variation in gene regulation may be common in nature, and might be of great evolutionary consequence; but the ascertainment of variation in gene regulation has proven to be a difficult problem. In this study, we explore this problem by measuring alcohol dehydrogenase (ADH) activity in Drosophila melanogaster strains homozygous for various combinations of given second and third chromosomes sampled from a natural population. The structural locus (Adh) coding for ADH is on the second chromosome. The results show that: (1) there are genes, other than Adh, that affect the levels of ADH activity; (2) at least some of these "regulatory" genes are located on the third chromosome, and thus are not adjacent to the Adh locus; (3) variation exists in natural populations for such regulatory genes; (4) the effect of these regulatory genes varies as they interact with different second chromosomes; (5) third chromosomes with high-activity genes are either partially or completely dominant over chromosomes with low-activity genes; (6) the effects of the regulatory genes are pervasive throughout development; and (7) the third chromosome genes regulate the levels of ADH activity by affecting the number of ADH molecules in the flies. The results are consistent with the view that the evolution of regulatory genes may play an important role in adaptation.

Study on Corrosion of X80 Pipeline Steel in Different Water Content of Soil

2014 ◽  
Vol 1015 ◽  
pp. 655-658
Author(s):  
Shu Zhen Yu ◽  
Guang Jun Xu ◽  
Han Hua Song ◽  
Xun Zhu ◽  
Wen We Lu ◽  
...  
Keyword(s):  
Water Content ◽  
High Frequency ◽  
Pipeline Steel ◽  
Electrochemical Corrosion ◽  
High Water ◽  
High Water Content ◽  
Linear Segment ◽  
X80 Pipeline Steel ◽  
The Third ◽  
In The Beginning

The electrochemical corrosion of X80 pipeline steel in Xinzhou’s soil with different water content is tested and analyzed. The corrosion signal time and frequency domain figure show that the corrosion signal fluctuates all the time in 30 days’ test with low water content (14%). The slope of high-frequency linear segment in the potential PSD is smaller than it is at the beginning. In the soil with the middle water content (18%), the intense wave motion lasts for 15 days. The noise fluctuation only exists before the third days when in the high water content soil (22%). After the third day, linear drift can be observed but no transient peak. The slope of high-frequency linear segment changes obviously compared with the situation in the beginning. And the high-frequency white noise appears.

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