scholarly journals Dense Genetic Linkage Maps of Three Populus Species (Populus deltoides, P. nigra and P. trichocarpa) Based on AFLP and Microsatellite Markers

Genetics ◽  
2001 ◽  
Vol 158 (2) ◽  
pp. 787-809 ◽  
Author(s):  
Maria-Teresa Cervera ◽  
Véronique Storme ◽  
Bart Ivens ◽  
Jaqueline Gusmão ◽  
Ben H Liu ◽  
...  
Keyword(s):  
Populus Deltoides ◽  
Female Parent ◽  
Aflp Markers ◽  
Linkage Maps ◽  
Important Species ◽  
Mapping Strategy ◽  
Sequence Tagged Sites ◽  
Fundamental Research ◽  
Populus Species ◽  
The Individual

Abstract Populus deltoides, P. nigra, and P. trichocarpa are the most important species for poplar breeding programs worldwide. In addition, Populus has become a model for fundamental research on trees. Linkage maps were constructed for these three species by analyzing progeny of two controlled crosses sharing the same female parent, Populus deltoides cv. S9-2 × P. nigra cv. Ghoy and P. deltoides cv. S9-2 × P. trichocarpa cv. V24. The two-way pseudotestcross mapping strategy was used to construct the maps. Amplified fragment length polymorphism (AFLP) markers that segregated 1:1 were used to form the four parental maps. Microsatellites and sequence-tagged sites were used to align homoeologous groups between the maps and to merge linkage groups within the individual maps. Linkage analysis and alignment of the homoeologous groups resulted in 566 markers distributed over 19 groups for P. deltoides covering 86% of the genome, 339 markers distributed over 19 groups for P. trichocarpa covering 73%, and 369 markers distributed over 28 groups for P. nigra covering 61%. Several tests for randomness showed that the AFLP markers were randomly distributed over the genome.

scholarly journals Construction of High-Density Linkage Maps of Populus deltoides × P. simonii Using Restriction-Site Associated DNA Sequencing

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0150692 ◽  
Author(s):  
Chunfa Tong ◽  
Huogen Li ◽  
Ying Wang ◽  
Xuran Li ◽  
Jiajia Ou ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Restriction Site ◽  
Populus Deltoides ◽  
High Density ◽  
Linkage Maps

AFLP targeting of the 1-cM region conferring the vrs1 gene for six-rowed spike in barley, Hordeum vulgare L.

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1122-1129 ◽  
Author(s):  
Congfen He ◽  
Badraldin Ebrahim Sayed-Tabatabaei ◽  
Takao Komatsuda
Keyword(s):  
Hordeum Vulgare ◽  
Fragment Length ◽  
Positional Cloning ◽  
Aflp Markers ◽  
Near Isogenic Lines ◽  
Hordeum Vulgare L ◽  
Plant Materials ◽  
Multiple Alleles ◽  
Isogenic Lines ◽  
Sequence Tagged Sites

Spike morphology is a key characteristic in the study of barley domestication, yield, and use. Multiple alleles at the vrs1 locus control the development and fertility of the lateral spikelets of barley. We developed five amplified fragment length polymorphism (AFLP) markers tightly linked to the vrs1 locus using well-characterized near-isogenic lines as plant materials. The AFLP markers were integrated into three different maps, in which 'Azumamugi' was used as the maternal parent. Of the three maps, Hordeum vulgare L. 'Azumamugi' × H. vulgare 'Golden Promise' showed recombination of the AFLP markers and the vrs1 locus (closest, 0.05 cM), providing the best mapping population for positional cloning of alleles at the vrs1 locus. Conversion of AFLP bands into polymorphic sequence-tagged sites (STSs) is necessary for further high-throughput genotype scoring and for bacterial artificial chromosome (BAC) library screening. We cloned and sequenced the five AFLP bands and synthesized primer pairs. PCR amplification generated DNAs of the same size from all four parental lines for each marker. Restriction endonuclease treatment of e40m36-1110/AccIII, e34m13-260/Psp1406I, e52m32-270/FokI, and e31m26-520/MnlI revealed fragment length polymorphisms between 'Azumamugi' and all the two-rowed parents. Allelism between the AFLPs and corresponding STS markers was confirmed genetically, indicating the usefulness of the STSs as genetic markers.Key words: positional cloning, codominance, near-isogenic lines, high-resolution maps, STSs.

Marker allozyme genes and alleles for differentiation of Populus deltoides, P. nigra, P. maximowiczii, and their interspecific hybrids

1990 ◽  
Vol 68 (5) ◽  
pp. 990-998 ◽  
Author(s):  
Om P. Rajora
Keyword(s):  
Interspecific Hybrids ◽  
Populus Deltoides ◽  
Single Pair ◽  
Enzyme Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Root Tips ◽  
Controlled Crosses ◽  
Populus Species ◽  
Species Specific ◽  
Allozyme Loci

Horizontal starch gel electrophoresis of enzymes was used to compare the allelic constitution of individuals of Populus deltoides Marsh., P. nigra L., P. maximowiczii Henry, P. ×canadensis Moench, and F1 progeny of controlled crosses. Forty allozyme loci coding for 12 enzyme systems in root tips were observed. Populus deltoides, P. nigra, and P. maximowiczii were genetically distinct from each other. Each of these species had unique alleles at many loci, and one or two of these species also had some species-specific genes. Populus deltoides, P. nigra, and P. maximowiczii could be distinguished by mutually exclusive or unique alleles at any of the four allozyme loci Aco-2, Lap-1, Lap-2, and Pgi-2. Additionally, allozymes of Pgm-1, 6-Pgd-2, 6-Pgd-4, and 6-Pgd-5 could be used as markers to distinguish P. deltoides from P. nigra and P. maximowiczii, allozymes of Mdh-2, Per-3, Pgm-2, and Pgm-3 to distinguish P. nigra from P. deltoïdes and P. maximowiczii, and allozymes of Got-1, Got-4, and Pgi-1 to distinguish P. maximowiczii from P. deltoides and P. nigra. The observed marker allozyme genes and alleles can be effectively used for discriminating among the three Populus species and their interspecific hybrids, and identification and verification of paternity of progeny of single-pair and interspecific pollen-mix controlled crosses. Biochemical and molecular markers have significance in genetics, breeding, and systematics of these Populus species. Key words: Populus, allozymes, diagnostic genes and alleles, species and hybrid differentiation, enzyme electrophoresis.

scholarly journals Asymmetrical natural hybridization between Populus deltoides and P. balsamifera (Salicaceae)This note is one of a selection of papers published in the Special Issue on Poplar Research in Canada.

2007 ◽  
Vol 85 (12) ◽  
pp. 1227-1232 ◽  
Author(s):  
Mona Hamzeh ◽  
Christina Sawchyn ◽  
Pierre Périnet ◽  
Selvadurai Dayanandan
Keyword(s):  
Populus Deltoides ◽  
Female Parent ◽  
Forest Tree ◽  
Snp Markers ◽  
Natural Hybridization ◽  
Populus Balsamifera ◽  
Chloroplast Genomes ◽  
Species Specific ◽  
Evolutionary Consequences ◽  
Species Being

Natural hybridization has long been recognized as a means for gene flow between species and has important evolutionary consequences. Although hybridization is generally considered to be symmetrical, with both hybridizing species being equally likely to be the male or female parent, several studies have demonstrated the presence of asymmetrical hybridization and introgression from one species to the other. We investigated the direction of natural hybridization between two sympatric forest tree species in North America ( Populus deltoides Bartr. ex Marsh. and Populus balsamifera L.) using species-specific single nucleotide polymorphism (SNP) markers in both the nuclear and chloroplast genomes. All natural hybrid individuals, identified from morphological traits, had nuclear alleles corresponding to both parental species, while the chloroplast genotypes showed similarity to P. deltoides, indicating asymmetrical hybridization with P. deltoides as the maternal and P. balsamifera as the paternal donor species. This observed asymmetrical hybridization may be attributable to cytonuclear interactions.

scholarly journals Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides ‘S9-2’

2001 ◽  
Vol 91 (11) ◽  
pp. 1069-1073 ◽  
Author(s):  
J. Zhang ◽  
M. Steenackers ◽  
V. Storme ◽  
S. Neyrinck ◽  
M. Van Montagu ◽  
...  
Keyword(s):  
Binding Site ◽  
Genetic Map ◽  
Positional Cloning ◽  
Populus Deltoides ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Aflp Markers ◽  
Interspecific Crosses ◽  
Leucine Rich Repeat ◽  
Starting Point

Melampsora larici-populina is the most damaging leaf pathogen for poplar in Europe. Previous genetic analyses have revealed both qualitative and quantitative resistance to this fungus. As a starting point for positional cloning of the gene or genes conferring qualitative resistance to M. larici-populina races E1, E2, and E3, a local genetic map of the Melampsora resistance (MER) locus was constructed based on amplified fragment length polymorphism (AFLP) markers. Eleven AFLP markers were identified by bulked segregant analysis. These markers were used to identify 17 recombinants at the MER locus, from a total of 512 progenies derived from three interspecific crosses involving the same resistant female parent, Populus deltoides ‘S9-2’. The local genetic map covered a 3.4-centimorgan interval encompassing the target locus. Sequence analysis of these AFLP markers revealed similarities to the nucleotide binding site/leucine-rich repeat class of disease resistance genes.

Two high-density AFLP® linkage maps of Zea mays L.: analysis of distribution of AFLP markers

1999 ◽  
Vol 99 (6) ◽  
pp. 921-935 ◽  
Author(s):  
M. Vuylsteke ◽  
R. Mank ◽  
R. Antonise ◽  
E. Bastiaans ◽  
M. L. Senior ◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
High Density ◽  
Aflp Markers ◽  
Linkage Maps

scholarly journals Growth performance of hybrid poplar clones on two agricultural sites with and without early irrigation and fertilization

2019 ◽  
Vol 68 (1) ◽  
pp. 58-66 ◽  
Author(s):  
Neil D. Nelson ◽  
Richard Meilan ◽  
William E. Berguson ◽  
Bernard G. McMahon ◽  
Meijun Cai ◽  
...  
Keyword(s):  
Populus Deltoides ◽  
Statistical Significance ◽  
Irrigation Treatment ◽  
Hybrid Poplar ◽  
Female Parent ◽  
Height Growth ◽  
Screening Tests ◽  
Environment Interaction ◽  
Genotype X Environment ◽  
Poplar Clones

Abstract Growth, clone rank, genotype x environment interaction, and effects of early fertilization and irrigation were evaluated for 56 hybrid poplar clones after three years’ growth on two agricultural sites in Indiana, USA. Forty-eight percent of the clones were Populus deltoides x P. nigra (DxN) crosses made with a female parent of Minnesota provenance, with the hybrids or female parent screened in Minnesota for survival, growth rate, and disease tolerance or resistance. Forty-one percent of the clones had at least one parent from provenances that are more southerly and/or have more moderate climates of provenance origin than Minnesota. Eleven percent of the clones were screened in Minnesota but were either not DXN crosses or did not have parents from Minnesota provenances in their parentage. Height growth averaged 1.78 m per year for all clones over all treatments and 2.02 m per year for the fastest- growing six clones (top 10th percentile). Tree bole volume for the fastest-growing 10 % of the clones was 70 % larger than the average of two commercial standard clones. The clonal effect was dominant in comparison to site, treatment, and interaction effects. The fertilizer, irrigation, and fertilizer x irrigation treatments tended to increase growth, but the statistical significance of the treatment effects differed by site, and the treatments explained only a small portion of the variance. Clone rank was the same on both sites, regardless of treatment, except for the fertilizer x irrigation treatment. DxN clones linked to Minnesota parentage out-performed most clones of more southerly or other more moderate climatic origins, in these tests conducted far south of Minnesota. The data provide further evidence of broad adaptability of DxN hybrids with female P. deltoides parents of Minnesota provenance, possibly eliminating the need for narrow breeding zones and reducing the number of screening tests needed at different latitudes, saving time and money. Tests are planned to further analyze and possibly extend this inference.

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