scholarly journals Cytogenetic analysis of chromosome region 73AD of Drosophila melanogaster.

Genetics ◽  
1990 ◽  
Vol 125 (4) ◽  
pp. 783-793
Author(s):  
J M Belote ◽  
F M Hoffmann ◽  
M McKeown ◽  
R L Chorsky ◽  
B S Baker
Keyword(s):  
Drosophila Melanogaster ◽  
Mutational Analysis ◽  
Chromosome Region ◽  
Temperature Sensitive ◽  
Recessive Lethal ◽  
Male And Female ◽  
Complementation Groups ◽  
Molecular Maps ◽  
Dominant Temperature Sensitive ◽  
Dark Body

Abstract The 73AD salivary chromosome region of Drosophila melanogaster was subjected to mutational analysis in order to (1) generate a collection of chromosome breakpoints that would allow a correlation between the genetic, cytological and molecular maps of the region and (2) define the number and gross organization of complementation groups within this interval. Eighteen complementation groups were defined and mapped to the 73A2-73B7 region, which is comprised of 17 polytene bands. These complementation groups include the previously known scarlet (st), transformer (tra) and Dominant temperature-sensitive lethal-5 (DTS-5) genes, as well as 13 new recessive lethal complementation groups and one male and female sterile locus. One of the newly identified lethal complementation groups corresponds to the molecularly identified abl locus, and another gene is defined by mutant alleles that exhibit an interaction with the abl mutants. We also recovered several mutations in the 73C1-D1.2 interval, representing two lethal complementation groups, one new visible mutant, plucked (plk), and a previously known visible, dark body (db). There is no evidence of a complex of sex determination genes in the region near tra.

scholarly journals MUTATIONAL ANALYSIS OF THE REGION SURROUNDING THE 93D HEAT SHOCK LOCUS OF DROSOPHILA MELANOGASTER

Genetics ◽  
1984 ◽  
Vol 106 (2) ◽  
pp. 249-265
Author(s):  
Jym Mohler ◽  
Mary Lou Pardue
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Mutational Analysis ◽  
Point Mutations ◽  
Γ Irradiation ◽  
Lethal Mutations ◽  
Complementation Groups ◽  
In Trans ◽  
Shock Locus ◽  
Shock Proteins

ABSTRACT The region containing subdivisions 93C, 93D and 93E on chromosome 3 of Drosophila melanogaster has been screened for visible and lethal mutations. Treatment with three mutagens, γ irradiation, ethyl methanesulfonate and diepoxybutane, has produced mutations that fall into 20 complementation groups, including the previously identified ebony locus. No point mutations affecting the heat shock locus in 93D were detected; however, a pair of deficiencies that overlap in the region of this locus was isolated. Flies heterozygous in trans for this pair of deficiencies are capable of producing all of the major heat shock puffs (except 93D) and the major heat shock proteins. In addition, these flies show recovery of normal protein synthesis following a heat shock.

scholarly journals Cytogenetic and molecular localization of tipE: a gene affecting sodium channels in Drosophila melanogaster.

Genetics ◽  
1995 ◽  
Vol 139 (4) ◽  
pp. 1679-1688 ◽  
Author(s):  
G Feng ◽  
P Deák ◽  
D P Kasbekar ◽  
D W Gil ◽  
L M Hall
Keyword(s):  
Drosophila Melanogaster ◽  
Sodium Channel ◽  
Sodium Channels ◽  
Positional Cloning ◽  
Chromosome Region ◽  
Translocation Breakpoint ◽  
Temperature Sensitive ◽  
Binding Studies ◽  
Sodium Permeability ◽  
Cloned Gene

Abstract Voltage-sensitive sodium channels play a key role in nerve cells where they are responsible for the increase in sodium permeability during the rising phase of action potentials. In Drosophila melanogaster a subset of temperature-sensitive paralytic mutations affect sodium channel function. One such mutation is temperature-induced paralysis locus E (tipE), which has been shown by electrophysiology and ligand binding studies to reduce sodium channel numbers. Three new gamma-ray-induced tipE alleles associated with either visible deletions in 64AB or a translocation breakpoint within 64B2 provide landmarks for positional cloning of tipE. Beginning with the flanking cloned gene Ras2, a 140-kb walk across the translocation breakpoint was completed. Germline transformation using a 42-kb cosmid clone and successively smaller subclones localized the tipE gene within a 7.4-kb genomic DNA segment. Although this chromosome region is rich in transcripts, only three overlapping mRNAs (5.4, 4.4, and 1.7 kb) lie completely within the smallest rescuing construct. The small sizes of the rescuing construct and transcripts suggest that tipE does not encode a standard sodium channel alpha-subunit with four homologous repeats. Sequencing these transcripts will elucidate the role of the tipE gene product in sodium channel functional regulation.

scholarly journals Genetic analysis of the adenosine3 (Gart) region of the second chromosome of Drosophila melanogaster.

Genetics ◽  
1990 ◽  
Vol 124 (4) ◽  
pp. 889-897
Author(s):  
S Y Tiong ◽  
D Nash
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Gene Product ◽  
Genetic Interaction ◽  
Chromosome Region ◽  
The Other ◽  
Purine Biosynthesis ◽  
Induced Mutations ◽  
Complementation Groups ◽  
Functional Complexity

Abstract The Gart gene of Drosophila melanogaster is known, from molecular biological evidence, to encode a polypeptide that serves three enzymatic functions in purine biosynthesis. It is located in polytene chromosome region 27D. One mutation in the gene (ade3(1)) has been described previously. We report here forty new ethyl methanesulfonate-induced mutations selected aga!nst a synthetic deficiency of the region from 27C2-9 to ++28B3-4. The mutations were characterized cytogenetically and by complementation analysis. The analysis apparently identifies 12 simple complementation groups. In addition, two segments of the chromosome exhibit complex complementation behavior. The first, the 28A region, gave three recessive lethals and also contains three known visible mutants, spade (spd), Sternopleural (Sp) and wingless (wg); a complex pattern of genetic interaction in the region incorporates both the new and the previously known mutants. The second region is at 27D, where seven extreme semilethal mutations give a complex complementation pattern that also incorporates ade3(1). Since ade3(1) is defective in one of the enzymatic functions encoded in the Gart gene, we assume the other seven also affect the gene. The complexity of the complementation pattern presumably reflects the functional complexity of the gene product. The phenotypic effects of the mutants at 27D are very similar to those described for ade2 mutations, which also interrupt purine biosynthesis.

scholarly journals Genetic and molecular mapping of chromosome region 85A in Drosophila melanogaster.

Genetics ◽  
1988 ◽  
Vol 120 (3) ◽  
pp. 733-742
Author(s):  
W K Jones ◽  
J M Rawls
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Molecular Mapping ◽  
Chromosome Region ◽  
Chromosome Rearrangements ◽  
Genetic Complementation ◽  
Chromosomal Segment ◽  
Molecular Map ◽  
Molecular Studies ◽  
Complementation Groups

Abstract Chromosome region 85A contains at least 12 genetic complementation groups, including the genes dhod, pink and hunchback. In order to better understand the organization of this chromosomal segment and to permit molecular studies of these genes, we have carried out a genetic analysis coupled with a chromosome walk to isolate the DNA containing these genes. Complementation tests with chromosomal deficiencies permitted unambiguous ordering of most of the complementation groups identified within the 85A region. Recombinant bacteriophage clones were isolated that collectively span over 120 kb of 85A DNA and these were used to produce a molecular map of the region. The breakpoint sites of a number of 85A chromosome rearrangements were localized on the molecular map, thereby delimiting regions of the DNA that contain the various genetic complementation groups.

scholarly journals Studies of the genetic organization of the vestigial microregion of Drosophila melanogaster.

Genetics ◽  
1988 ◽  
Vol 120 (2) ◽  
pp. 495-502
Author(s):  
P F Lasko ◽  
M L Pardue
Keyword(s):  
Drosophila Melanogaster ◽  
Recessive Lethal ◽  
Genetic Organization ◽  
Complementation Groups

Abstract The region of the second chromosome of Drosophila melanogaster defined by Df(2R)vgB was screened for recessive lethal and visible mutations. Fifty-eight new recessive alleles fall into 17 complementation groups. Many new vg alleles were also isolated in a screen for new vg deficiencies. The breakpoints of the new vg deficiencies were nonrandomly distributed. The distal breakpoints of twelve of 20 deficiencies overlapping Df(2R)vgB are genetically identical to that of Df(2R)vgD, coinciding with the position of a complex, pleiotropic locus, l(2)49Ea-Psc-Su(z)2.

scholarly journals Genetic and Bioinformatic Analysis of 41C and the 2R Heterochromatin of Drosophila melanogaster: A Window on the Heterochromatin-Euchromatin Junction

Genetics ◽  
2004 ◽  
Vol 166 (2) ◽  
pp. 807-822 ◽  
Author(s):  
Steven H Myster ◽  
Fei Wang ◽  
Robert Cavallo ◽  
Whitney Christian ◽  
Seema Bhotika ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Large Scale ◽  
Physical Map ◽  
Chromosome Region ◽  
Bioinformatic Analysis ◽  
Pericentric Heterochromatin ◽  
Genome Project ◽  
Essential Genes ◽  
Complementation Groups ◽  
The Right

Abstract Genomic sequences provide powerful new tools in genetic analysis, making it possible to combine classical genetics with genomics to characterize the genes in a particular chromosome region. These approaches have been applied successfully to the euchromatin, but analysis of the heterochromatin has lagged somewhat behind. We describe a combined genetic and bioinformatics approach to the base of the right arm of the Drosophila melanogaster second chromosome, at the boundary between pericentric heterochromatin and euchromatin. We used resources provided by the genome project to derive a physical map of the region, examine gene density, and estimate the number of potential genes. We also carried out a large-scale genetic screen for lethal mutations in the region. We identified new alleles of the known essential genes and also identified mutations in 21 novel loci. Fourteen complementation groups map proximal to the assembled sequence. We used PCR to map the endpoints of several deficiencies and used the same set of deficiencies to order the essential genes, correlating the genetic and physical map. This allowed us to assign two of the complementation groups to particular “computed/curated genes” (CGs), one of which is Nipped-A, which our evidence suggests encodes Drosophila Tra1/TRRAP.

scholarly journals Genetic analysis of chromosome region 63 of Drosophila melanogaster.

Genetics ◽  
1991 ◽  
Vol 128 (4) ◽  
pp. 763-775 ◽  
Author(s):  
A D Wohlwill ◽  
J J Bonner
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Chromosome Region ◽  
P Element ◽  
Cytogenetic Mapping ◽  
Lethal Mutations ◽  
Complementation Groups

Abstract The salivary chromosome region including cytological division 63 of Drosophila melanogaster was genetically analyzed in order to (1) characterize this previously unstudied region and (2) attempt to isolate mutations in the hsp82 gene. Seven deletions which span this region were isolated, including four which remove the hsp82 gene. A Minute mutation was mapped to this region and this Minute was used to isolate duplications in the 63 region. These duplications map the Minute to 63B8-C1. F2 screens were initiated using deletions which remove the hsp82 gene. Over 15,000 chromosomes were screened, yielding 40 lethal mutations which comprise 14 complementation groups. Several of these mutations map outside the 63 region and appear to give second site interaction with the Minute locus. Four loci, including the Minute gene, are candidates for hsp82 mutations by cytogenetic mapping. These loci were tested for complementation with a P element carrying the hsp82 gene. However, none of the mutations was rescued.

Genetic analysis of the foraging microregion of Drosophila melanogaster

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 94-101 ◽  
Author(s):  
J. Steven de Belle ◽  
Marla B. Sokolowski ◽  
Arthur J. Hilliker
Keyword(s):  
Drosophila Melanogaster ◽  
Mutational Analysis ◽  
Allelic Variation ◽  
Larval Behaviour ◽  
Naturally Occurring ◽  
Complementation Groups ◽  
Behavioural Phenotypes ◽  
Genetically Determined ◽  
Behaviour Genetics ◽  
New Mutations

The rover/sitter polymorphism in Drosophila melanogaster larval behaviour is a unique example of a genetically determined, naturally occurring behavioural polymorphism. Allelic variation at the foraging locus (for) accounts for the rover (long foraging paths) and sitter (short foraging paths) phenotypes. We previously developed lethal tagging and used deficiency mapping to place for in the 24A3-C5 interval on the polytene chromosome map, thereby defining the for microregion. Here, we subjected this microregion to mutational analysis to (i) isolate putative lethal foraging mutations and characterize their behavioural phenotypes to assess whether or not for is a vital locus, (ii) generate cytologically detectable chromosome rearrangements with breakpoints in or near for for more precise localization and for future molecular analysis of the for gene, and (iii) identify other gene loci in the immediate vicinity of the for locus. We recovered 10 gamma-induced and 33 ethyl methanesulfonate (EMS) induced new mutations that define seven complementation groups in 24A3-D4. Two new EMS-induced lethal for alleles and four gamma-induced rearrangements with breakpoints in for were identified, which allowed us to further localize for to 24A3-5. All lethal mutations in for resulted in an altered behavioural phenotype providing evidence that both vital and behavioural functions are encoded by for.Key words: behaviour, genetics, foraging microregion, Drosophila melanogaster, larvae.

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