scholarly journals DNA SEQUENCE COMPARISON AMONG CLOSELY RELATED DROSOPHILA SPECIES IN THE MULLERI COMPLEX

Genetics ◽  
1986 ◽  
Vol 113 (2) ◽  
pp. 287-303
Author(s):  
Dan H Schulze ◽  
C S Lee
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Single Copy ◽  
Drosophila Species ◽  
Base Pairs ◽  
Drosophila Mulleri ◽  
Copy Dna ◽  
Sequence Relationships ◽  
Additional Member ◽  
Single Copy Dna

ABSTRACT DNA hybridization was used to establish DNA sequence relationships among seven Drosophila species. Single-copy DNA was isolated from four species within the Drosophila mulleri complex, D. mojavensis, D. arizonensis, D. ritae and D. starmeri. These single-copy DNAs were used as tracers to be hybridized with each other and one additional member of the mulleri complex, D. aldrichi, a member of a closely related complex, D. hydei, and a distantly related species, D. melanogaster. Two methods have been used to determine the relatedness between these species: (1) the extent of duplex formed as measured by binding to hydroxyapatite and (2) the thermal stability of the duplexed DNA. Moderately repetitive DNA was purified from these species and used similarly to determine the divergence of this family of sequences. The rate of nucleotide substitution was estimated to be 0.2 ± 0.1% base pair change per million years for both single-copy and middle-repetitive DNAs. The size of the D. arizonensis genome, a representative of the mulleri complex, was calculated to be 2.2 × 108 base pairs from its kinetic complexity similar to that of D. hydei. The relative amounts (18%) and average reiteration frequency (100 copies) of the middle-repetitive DNA are similar for all Drosophila species studied. Finally, the data are presented in a phylogenetic tree.

Physical localization of single-copy sequences on pachytene chromosomes in maize (Zea mays L.) by chromosome in situ suppression hybridization

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 1081-1083 ◽  
Author(s):  
Monther T Sadder ◽  
Norbert Ponelies ◽  
Ute Born ◽  
Gerd Weber
Keyword(s):  
Zea Mays ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Sequence ◽  
Zea Mays L ◽  
Single Copy ◽  
Chromosome 9 ◽  
Copy Dna ◽  
Single Copy Dna

A new approach for locating single-copy DNA sequences on pachytene chromosomes of maize (Zea mays L.) was developed. A cosmid clone with homologous sequences to a molecular marker (umc105a) linked to a quantitative trait locus (QTL) for resistance against sugarcane borer (SCB) was physically mapped by fluorescence in situ hybridization (FISH) to the short arm of chromosome 9. The marker umc105a was genetically placed in the centromeric region. To suppress signals generated by maize repetitive DNA, competitive in situ suppression (CISS) hybridization was necessary to obtain specific signals from umc105a. A centromere specific DNA probe (CentC) was used in a double-labeling technique as a reference marker. Fluorescence signals generated by umc105a cosmid and CentC were specific and highly reproducible. Thus the single-copy DNA sequence of umc105a was physically localized on the short arm of chromosome 9 near the telomere. This is the first report of physical localization of single-copy DNA sequence by CISS hybridization to a maize pachytene chromosome.Key words: fluorescence in situ hybridization, maize, pachytene chromosome, single-copy sequence, CISS hybridization.

scholarly journals An arbitrary single copy DNA sequence VC85 [D1S85] detects a 500 bp insertion/deletion polymorphism on chromosome 1

1989 ◽  
Vol 17 (13) ◽  
pp. 5420-5420 ◽  
Author(s):  
Vera Verga ◽  
Maria Dos Santos ◽  
Isabelle Marques ◽  
Sue Povey ◽  
Michele Ramsay ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Single Copy ◽  
Chromosome 1 ◽  
Deletion Polymorphism ◽  
Copy Dna ◽  
Single Copy Dna

Sequence organization and developmental expression of an interspersed, repetitive element and associated single-copy DNA sequences in Dictyostelium discoideum

1985 ◽  
Vol 5 (8) ◽  
pp. 2123-2130
Author(s):  
A R Kimmel ◽  
R A Firtel
Keyword(s):  
Dna Sequences ◽  
Transcription Initiation ◽  
Sequence Data ◽  
Single Copy ◽  
Developmental Expression ◽  
Cdna Clones ◽  
Base Pairs ◽  
Sequence Element ◽  
Copy Dna ◽  
Single Copy Dna

We have examined the genomic organization and developmental expression pattern of a short, transcribed, interspersed repeat element and its associated single-copy sequences. We have previously shown that 1% of the polyadenylated [poly(A)+] RNA from vegetative cells contains sequences that hybridize to this repeat. The complementary RNA is heterogeneous in size, and 90% of its mass hybridizes to single-copy DNA. In this study, we examined a series of genomic DNAs and cDNAs derived from poly(A)+ RNAs which are complementary to the repeat. Comparisons of sequence data from various genomic and cDNA clones indicated that (AAC)n X (GTT)n is the common sequence element. The tandem repeat occurred in approximately 100 short segments (approximately 35 to 150 base pairs) per haploid genome interspersed with single-copy DNA. Probes from regions adjacent to this element hybridized to unique restriction fragments on DNA blots and unique poly(A)+ RNA species on RNA blots. The (AAC)n X (GTT)n sequence was asymmetrically transcribed with only (AAC)n sequences represented in RNA. The repeat was localized within the transcribed regions of several genes and 70 base pairs 5' to the transcription initiation site of another gene. Individual (AAC)n-containing RNAs exhibited a developmental pattern of expression suggestive of the coordinate expression of many AAC gene family members.

scholarly journals Sequence organization and developmental expression of an interspersed, repetitive element and associated single-copy DNA sequences in Dictyostelium discoideum.

1985 ◽  
Vol 5 (8) ◽  
pp. 2123-2130 ◽  
Author(s):  
A R Kimmel ◽  
R A Firtel
Keyword(s):  
Dna Sequences ◽  
Transcription Initiation ◽  
Sequence Data ◽  
Single Copy ◽  
Developmental Expression ◽  
Cdna Clones ◽  
Base Pairs ◽  
Sequence Element ◽  
Copy Dna ◽  
Single Copy Dna

We have examined the genomic organization and developmental expression pattern of a short, transcribed, interspersed repeat element and its associated single-copy sequences. We have previously shown that 1% of the polyadenylated [poly(A)+] RNA from vegetative cells contains sequences that hybridize to this repeat. The complementary RNA is heterogeneous in size, and 90% of its mass hybridizes to single-copy DNA. In this study, we examined a series of genomic DNAs and cDNAs derived from poly(A)+ RNAs which are complementary to the repeat. Comparisons of sequence data from various genomic and cDNA clones indicated that (AAC)n X (GTT)n is the common sequence element. The tandem repeat occurred in approximately 100 short segments (approximately 35 to 150 base pairs) per haploid genome interspersed with single-copy DNA. Probes from regions adjacent to this element hybridized to unique restriction fragments on DNA blots and unique poly(A)+ RNA species on RNA blots. The (AAC)n X (GTT)n sequence was asymmetrically transcribed with only (AAC)n sequences represented in RNA. The repeat was localized within the transcribed regions of several genes and 70 base pairs 5' to the transcription initiation site of another gene. Individual (AAC)n-containing RNAs exhibited a developmental pattern of expression suggestive of the coordinate expression of many AAC gene family members.

Cloning of a single-copy DNA sequence unique toPlasmodiophora brassicae

1997 ◽  
Vol 50 (5) ◽  
pp. 289-300 ◽  
Author(s):  
S. Ito ◽  
T. Maehara ◽  
S. Tanaka ◽  
M. Kameya-Iwaki ◽  
S. Yano ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Single Copy ◽  
Copy Dna ◽  
Single Copy Dna

A restriction map of the ribosomal RNA genes and the short single-copy DNA sequence of the pearl millet chloroplast genome

Gene ◽  
1981 ◽  
Vol 16 (1-3) ◽  
pp. 11-19 ◽  
Author(s):  
James R.Y. Rawson ◽  
Michael T. Clegg ◽  
Karen Thomas ◽  
Claire Rinehart ◽  
Betty Wood
Keyword(s):  
Chloroplast Genome ◽  
Pearl Millet ◽  
Dna Sequence ◽  
Ribosomal Rna ◽  
Single Copy ◽  
Ribosomal Rna Genes ◽  
Restriction Map ◽  
Copy Dna ◽  
Rna Genes ◽  
Single Copy Dna

scholarly journals An arbitrary single copy DNA sequence VC85 [D1S85] detects a 500 bp insertion/deletion polymorphism on chromosome 1

1989 ◽  
Vol 17 (10) ◽  
pp. 4007-4007 ◽  
Author(s):  
Vera Verga ◽  
Maria Dos Santos ◽  
Isabelle Marques ◽  
Sue Povey ◽  
Michele Ramsay ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Single Copy ◽  
Chromosome 1 ◽  
Deletion Polymorphism ◽  
Copy Dna ◽  
Single Copy Dna
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