SINGLE-COPY DNA SEQUENCE DIVERGENCE IN TELEOSTEAN FISH SPECIES

1981 ◽  
Vol 9 (2) ◽  
pp. 266P-266P
Author(s):  
J. Schmidtke
Keyword(s):  
Dna Sequence ◽  
Fish Species ◽  
Sequence Divergence ◽  
Single Copy ◽  
Teleostean Fish ◽  
Copy Dna ◽  
Single Copy Dna

scholarly journals Rates of DNA evolution in Drosophila depend on function and developmental stage of expression.

Genetics ◽  
1993 ◽  
Vol 133 (2) ◽  
pp. 291-298
Author(s):  
J R Powell ◽  
A Caccone ◽  
J M Gleason ◽  
L Nigro
Keyword(s):  
Dna Hybridization ◽  
Developmental Stages ◽  
Sequence Data ◽  
Sequence Divergence ◽  
Single Copy ◽  
Evolutionary Divergence ◽  
Cdna Sequences ◽  
Copy Dna ◽  
Dna 2 ◽  
Single Copy Dna

Abstract DNA-sequence divergence of genes expressed in the embryonic stage was compared with the divergence of genes expressed in adults for 13 species of Drosophila representing various degrees of relatedness. DNA-DNA hybridization experiments were conducted using as tracers complementary DNA (cDNA) reversed transcribed from poly(A)+ mRNA isolated from different developmental stages. The results indicate: (1) cDNA is less diverged than total single-copy DNA; (2) cDNA sequences are not in the rapidly evolving fraction of the single-copy genome of Drosophila; (3) early in evolutionary divergence embryonic messages are about half as diverged as adult messages; sequence data from some of the species compared indicate this is likely due to differences in rates of silent substitutions in genes expressed at different stages of development; and (4) at greater evolutionary distance, the differences in embryonic and adult messages disappear; this could be due to lineage-specific shifts in codon usage.

Physical localization of single-copy sequences on pachytene chromosomes in maize (Zea mays L.) by chromosome in situ suppression hybridization

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 1081-1083 ◽  
Author(s):  
Monther T Sadder ◽  
Norbert Ponelies ◽  
Ute Born ◽  
Gerd Weber
Keyword(s):  
Zea Mays ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Sequence ◽  
Zea Mays L ◽  
Single Copy ◽  
Chromosome 9 ◽  
Copy Dna ◽  
Single Copy Dna

A new approach for locating single-copy DNA sequences on pachytene chromosomes of maize (Zea mays L.) was developed. A cosmid clone with homologous sequences to a molecular marker (umc105a) linked to a quantitative trait locus (QTL) for resistance against sugarcane borer (SCB) was physically mapped by fluorescence in situ hybridization (FISH) to the short arm of chromosome 9. The marker umc105a was genetically placed in the centromeric region. To suppress signals generated by maize repetitive DNA, competitive in situ suppression (CISS) hybridization was necessary to obtain specific signals from umc105a. A centromere specific DNA probe (CentC) was used in a double-labeling technique as a reference marker. Fluorescence signals generated by umc105a cosmid and CentC were specific and highly reproducible. Thus the single-copy DNA sequence of umc105a was physically localized on the short arm of chromosome 9 near the telomere. This is the first report of physical localization of single-copy DNA sequence by CISS hybridization to a maize pachytene chromosome.Key words: fluorescence in situ hybridization, maize, pachytene chromosome, single-copy sequence, CISS hybridization.

scholarly journals An arbitrary single copy DNA sequence VC85 [D1S85] detects a 500 bp insertion/deletion polymorphism on chromosome 1

1989 ◽  
Vol 17 (13) ◽  
pp. 5420-5420 ◽  
Author(s):  
Vera Verga ◽  
Maria Dos Santos ◽  
Isabelle Marques ◽  
Sue Povey ◽  
Michele Ramsay ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Single Copy ◽  
Chromosome 1 ◽  
Deletion Polymorphism ◽  
Copy Dna ◽  
Single Copy Dna

Cloning of a single-copy DNA sequence unique toPlasmodiophora brassicae

1997 ◽  
Vol 50 (5) ◽  
pp. 289-300 ◽  
Author(s):  
S. Ito ◽  
T. Maehara ◽  
S. Tanaka ◽  
M. Kameya-Iwaki ◽  
S. Yano ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Single Copy ◽  
Copy Dna ◽  
Single Copy Dna

A restriction map of the ribosomal RNA genes and the short single-copy DNA sequence of the pearl millet chloroplast genome

Gene ◽  
1981 ◽  
Vol 16 (1-3) ◽  
pp. 11-19 ◽  
Author(s):  
James R.Y. Rawson ◽  
Michael T. Clegg ◽  
Karen Thomas ◽  
Claire Rinehart ◽  
Betty Wood
Keyword(s):  
Chloroplast Genome ◽  
Pearl Millet ◽  
Dna Sequence ◽  
Ribosomal Rna ◽  
Single Copy ◽  
Ribosomal Rna Genes ◽  
Restriction Map ◽  
Copy Dna ◽  
Rna Genes ◽  
Single Copy Dna

scholarly journals An arbitrary single copy DNA sequence VC85 [D1S85] detects a 500 bp insertion/deletion polymorphism on chromosome 1

1989 ◽  
Vol 17 (10) ◽  
pp. 4007-4007 ◽  
Author(s):  
Vera Verga ◽  
Maria Dos Santos ◽  
Isabelle Marques ◽  
Sue Povey ◽  
Michele Ramsay ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Single Copy ◽  
Chromosome 1 ◽  
Deletion Polymorphism ◽  
Copy Dna ◽  
Single Copy Dna

Bovine thymus satellite I DNA sequences, which are highly methylated, are preferentially located in H1-rich chromatin

1988 ◽  
Vol 66 (4) ◽  
pp. 256-261 ◽  
Author(s):  
James R. Davie ◽  
Genevieve P. Delcuve
Keyword(s):  
Dna Methylation ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Single Copy ◽  
Nucleosomal Dna ◽  
Copy Dna ◽  
Single Copy Dna

The distribution of 5-methylcytosine among H1-rich and -poor bovine thymus chromatin regions was determined. 5-Methylcytosine was enriched in H1-rich chromatin regions, with linker and nucleosomal DNA containing similar amounts of this modified base. Satellite I DNA sequences, which constitute 5–7% of the genome and are highly methylated, were preferentially localized among H1-rich chromatin regions, in accordance with the distribution of 5-methylcytosine. In contrast to the satellite I DNA sequences, prothrombin (a single copy DNA sequence) was localized among both H1-rich and -poor chromatin regions. The results of this study are consistent with the hypothesis that DNA methylation has a role in modulating the structure of chromatin.

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