scholarly journals USE OF NYSTATIN-RESISTANT MUTATIONS IN PARASEXUAL GENETIC ANALYSIS IN DICTYOSTELIUM DISCOIDEUM

Genetics ◽  
1983 ◽  
Vol 104 (2) ◽  
pp. 271-277
Author(s):  
Durgadas P Kasbekar ◽  
Sanford Madigan ◽  
Eugene R Katz
Keyword(s):  
Linkage Group ◽  
Genetic Analysis ◽  
Dictyostelium Discoideum ◽  
Resistance Genes ◽  
Nutrient Agar ◽  
Group V ◽  
Complementation Groups ◽  
Extreme Sensitivity ◽  
Selection Of ◽  
Nystatin Resistance

ABSTRACT Nystatin-resistant mutations exhibit extreme sensitivity to 1.3 mm coumarin. The mutations fall into three complementation groups so it is possible to select for nonallelic mutations conferring sensitivity to coumarin by selection on nystatin-containing nutrient agar plates. Complementation between such coumarin-sensitive mutations allows the selection of diploids on coumarin-containing nutrient agar. Two of the nystatin resistance genes, nysB and nysC, have been mapped tentatively to the previously unmarked linkage group V.

scholarly journals LINKAGE ANALYSIS OF NYSTATIN RESISTANCE MUTATIONS IN DICTYOSTELIUM DISCOIDEUM

Genetics ◽  
1986 ◽  
Vol 113 (1) ◽  
pp. 53-62
Author(s):  
Dennis L Welker
Keyword(s):  
Linkage Group ◽  
Linkage Analysis ◽  
Dictyostelium Discoideum ◽  
Group Iv ◽  
Resistance Locus ◽  
Resistance Mutations ◽  
Group Vi ◽  
Group V ◽  
The Third ◽  
Nystatin Resistance

ABSTRACT Earlier linkage analyses of nystatin resistance loci in Dictyostelium discoideum tentatively mapped the nysB and nysC loci to the previously unmarked linkage group V. The data presented here establishes that nysB maps to linkage group VI and that nysC maps to linkage group IV. The third nystatin resistance locus, nysA, maps to linkage group II.

Mitotic mapping in linkage group V of Aspergillus niger based on selection of auxotrophic recombinants by Novozym enrichment

1989 ◽  
Vol 35 (11) ◽  
pp. 982-988 ◽  
Author(s):  
Alfons J. M. Debets ◽  
Klaas Swart ◽  
Cees J. Bos
Keyword(s):  
Aspergillus Niger ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Chromosome Mapping ◽  
Crossing Over ◽  
Diploid Strain ◽  
Group V ◽  
Clonal Distribution ◽  
Homozygous Diploid ◽  
Selection Of

This paper describes a procedure which allows the quantitative selection of auxotrophs of the fungus Aspergillus niger by enzymatic killing of immobilized germinating prototrophic conidiospores. We have applied this procedure to linkage analysis on the basis of mitotic crossing-over in this fungus. Starting with a heterozygous diploid strain, we could select auxotrophic homozygous diploid recombinants quantitatively. We estimated the frequency of crossing-over after correction for clonal distribution of recombinants, and localized four auxotrophic markers as well as the centromere on chromosome V of this fungus. The Novozym enrichment procedure proved to be useful in genetic analysis and for the construction of recombinant genotypes in the case of closely linked auxotrophic markers. The detemination of gene order and the estimation of distances on the basis of benomyl-induced recombinant haploid segregants may lead to incorrect conclusions. Genetic analysis on the basis of homozygous recombinants, however, can provide reliable estimates of map distances.Key words: Aspergillus niger, chromosome mapping, mitotic crossing-over, Novozym enrichment, auxotrophic recombinants.

scholarly journals THE SELECTION OF S. CEREVISIAE MUTANTS DEFECTIVE IN THE START EVENT OF CELL DIVISION

Genetics ◽  
1980 ◽  
Vol 95 (3) ◽  
pp. 561-577 ◽  
Author(s):  
Steven I Reed
Keyword(s):  
Cell Division ◽  
Genetic Analysis ◽  
Linkage Map ◽  
Nuclear Genes ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Mating Pheromone ◽  
Preliminary Characterization ◽  
Complementation Groups ◽  
Selection Of

ABSTRACT Thirty-three temperature-sensitive mutations defective in the start event of the cell division cycle of Saccharomyces cereuisiae were isolated and subjected to preliminary characterization. Complementation studies assigned these mutations to four complementation groups, one of which, cdc28, has been described previously. Genetic analysis revealed that these complementation groups define single nuclear genes, unlinked to one another. One of the three newly identified genes, cdc37, has been located in the yeast linkage map on chromosome IV, two meiotic map units distal to hom2.—Each mutation produces stage-specific arrest of cell division at start, the same point where mating pheromone interrupts division. After synchronization at start by incubation at the restrictive temperature, the mutants retain the capacity to enlarge and to conjugate.

PARASEXUAL GENETIC ANALYSIS OF CELL PROPORTIONING MUTANTS OF DICTYOSTELIUM DISCOIDEUM

Genetics ◽  
1981 ◽  
Vol 99 (2) ◽  
pp. 183-196
Author(s):  
James H Morrissey ◽  
William F Loomis
Keyword(s):  
Cell Differentiation ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Dictyostelium Discoideum ◽  
Double Mutant ◽  
Complementation Group ◽  
Stalk Cell ◽  
Recessive Mutation ◽  
Group Vi ◽  
Recessive Mutations

ABSTRACT Eight independently isolated mutants of Dictyostelium discoideum that differentiate exclusively into stalk cells make up one complementation group and carry single recessive mutations at the stalky locus, stkA, located on linkage group II. KY19, a previously described strain that differentiates into spores, but not stalk cells, was found to possess a recessive mutation defining the stalkless locus, stlA, located on linkage group VI. An analysis of the properties of these mutants, together with the phenotype of a haploid double mutant carrying stkA and stlA indicates that stlA results in poorly organized stalk tubes and incomplete stalk cell differentiation, while stkA causes all of the cells to differentiate into stalk cells, even when not enclosed in the stalk tube. The significance of these results is discussed in relation to current theories of pattern formation in D. discoideum.

scholarly journals The two-way selection of mutants and revertants in respect of acetate utilization and resistance to fluoro-acetate in Aspergillus nidulans

1965 ◽  
Vol 6 (3) ◽  
pp. 317-329 ◽  
Author(s):  
David Apirion
Keyword(s):  
Linkage Group ◽  
Aspergillus Nidulans ◽  
Group Vi ◽  
Major Pathway ◽  
Group V ◽  
Mutant Strains ◽  
Other Substances ◽  
Acetate Utilization ◽  
A Minor ◽  
Selection Of

An extension of two-way selection (i.e. selection of mutant from wild-type and vice versa within the same locus and with the same efficiency) to four different mutational or segregational situations was made possible by using acetate, fluoro-acetate and other substances related to their metabolism.Two types of mutants resistant to fluoro-acetate were selected, the first of which (designated fac) cannot grow on acetate as the sole carbon source, while the second (designated fan) can.Commencing with either a fac or a fan strain a double fac fan strain may be isolated, which is much more resistant to fluoro-acetate than either single mutant strain. Such double mutant strains may also be obtained by crossing a fac to a fan strain. Various characteristics of growth response of these strains on various media were observed.fac mutants are recessive and map in three meiotically unlinked loci, one in linkage group V and two in linkage group VIII.fan mutants are recessive and map in five loci, one in each of the linkage groups V, VII and VIII, and two linked in linkage group VI.Most fac mutants isolated did not revert and this failure is considered genuine. Of the revertants tested, most resulted from extra-cistron suppressors, while revertants of two fac mutants resulted from very closely linked or intra-cistron suppressors.It is argued that the findings indicate the existence of two pathways for acetate utilization in Aspergillus nidulans, a major and a minor; fac mutants are blocked in the major pathway, fan mutants in the minor pathway.

scholarly journals GENETIC ANALYSIS OF THE GENE FOR N-ACETYLGLUCOSAMINIDASE IN DICTYOSTELIUM DISCOIDEUM

Genetics ◽  
1978 ◽  
Vol 88 (2) ◽  
pp. 277-284 ◽  
Author(s):  
William F Loomis
Keyword(s):  
Linkage Group ◽  
Genetic Analysis ◽  
Dictyostelium Discoideum ◽  
Structural Gene ◽  
Regulatory Protein ◽  
Group Iv ◽  
Single Locus ◽  
Stages Of Development ◽  
Separate Locus

ABSTRACT Three independent mutations affecting N-acetylglucosaminidase in Dictyostelium discoideum were mapped by the parasexual system and found to lie on linkage group IV. These mutations as well as two others were found to be recessive and noncomplementing in heterozygous diploids. Thus they all appear to affect the nagA locus. Since two of the mutations give rise to thermolabile enzyme, this defines the structural gene for N-acetylglucosaminidase. The enzyme is a homodimer of a 68,000 dalton subunit and thus would be expected to be determined by a single locus. The expression of this gene is regulated by the stages of development; however, it should be mentioned that none of the mutations fell in a separate locus that might determine a specific positive regulatory protein.

Hansenula polymorpha as a novel yeast system for the expression of heterologous genes

1988 ◽  
Vol 16 (6) ◽  
pp. 1081-1083 ◽  
Author(s):  
P. E. SUDBERY ◽  
M. A. GLEESON ◽  
R. A. VEALE ◽  
A. M. LEDEBOER ◽  
M. C. M. ZOETMULDER
Keyword(s):  
Linkage Group ◽  
Genetic Analysis ◽  
Hansenula Polymorpha ◽  
Expression System ◽  
Carbon Sources ◽  
Neomycin Phosphotransferase ◽  
Yeast Expression System ◽  
Methanol Oxidase ◽  
Complementation Groups ◽  
New Yeast

Summary The advantages of Hansenula polymorpha as a new yeast expression system are discussed in terms of the powerful and regulatable methanol oxidase promoter and the organism's ability to grow on cheap carbon sources. The development of techniques for conventional genetic analysis is described. A total of 218 mutants have been assigned to 62 complementation groups, three genes have been found to be linked forming the first linkage group in this organism. Methods for molecular transformation have been developed allowing the expression of heterologous genes. The disruptive integration and expression of the neomycin phosphotransferase is described.

scholarly journals Genomic organization in Caenorhabditis elegans: deficiency mapping on linkage group V(left)

1988 ◽  
Vol 52 (2) ◽  
pp. 105-118 ◽  
Author(s):  
Raja E. Rosenbluth ◽  
Teresa M. Rogalski ◽  
Robert C. Johnsen ◽  
Linda M. Addison ◽  
David L. Baillie
Keyword(s):  
Caenorhabditis Elegans ◽  
Linkage Group ◽  
Mutant Allele ◽  
Genomic Organization ◽  
Group V ◽  
Recessive Lethal ◽  
C Elegans ◽  
Lethal Mutations ◽  
Autosomal Region ◽  
Complementation Groups

SummaryIn this study we genetically analyse a large autosomal region (23 map units) in Caenorhabditis elegans. The region comprises the left half of linkage group V [LGV(left)] and is recombinationally balanced by the translocation eT1(III; V). We have used rearrangement breakpoints to subdivide the region from the left end of LGV to daf-11 into a set of 23 major zones. Twenty of these zones are balanced by eT1. To establish the zones we examined a total of 110 recessive lethal mutations derived from a variety of screening protocols. The mutations identified 12 deficiencies, 1 duplication, as well as 98 mutations that fell into 59 complementation groups, significantly increasing the number of available genetic sites on LGV. Twenty-six of the latter had more than 1 mutant allele. Significant differences were observed among the alleles of only 6 genes, 3 of which have at least one ‘visible’ allele. Several deficiencies and 3 alleles of let-336 were demonstrated to affect recombination. The duplication identified in this study is sDp30(V;X). Lethal mutations covered by sDp30 were not suppressed uniformly in hermaphrodites. The basis for this non-uniformity may be related to the mechanism of X chromosome dosage compensation in C. elegans.

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