scholarly journals The marine sulfate reducer Desulfobacterium autotrophicum HRM2 can switch between low and high apparent half-saturation constants for dissimilatory sulfate reduction

2017 ◽  
Vol 93 (4) ◽  
Author(s):  
Irene Harder Tarpgaard ◽  
Bo Barker Jørgensen ◽  
Kasper Urup Kjeldsen ◽  
Hans Røy
Keyword(s):  
Sulfate Reduction ◽  
Dissimilatory Sulfate Reduction ◽  
Sulfate Reducer ◽  
Desulfobacterium Autotrophicum

scholarly journals Pseudodesulfovibrio cashew sp. Nov., a Novel Deep-Sea Sulfate-Reducing Bacterium, Linking Heavy Metal Resistance and Sulfur Cycle

2021 ◽  
Vol 9 (2) ◽  
pp. 429
Author(s):  
Rikuan Zheng ◽  
Shimei Wu ◽  
Chaomin Sun
Keyword(s):  
Heavy Metal ◽  
Sulfate Reduction ◽  
Marine Sediments ◽  
Deep Sea ◽  
Sulfur Cycle ◽  
Metal Sulfides ◽  
Phenotypic Traits ◽  
Dissimilatory Sulfate Reduction ◽  
Metabolic Potential ◽  
Sulfate Reducing

Sulfur cycling is primarily driven by sulfate reduction mediated by sulfate-reducing bacteria (SRB) in marine sediments. The dissimilatory sulfate reduction drives the production of enormous quantities of reduced sulfide and thereby the formation of highly insoluble metal sulfides in marine sediments. Here, a novel sulfate-reducing bacterium designated Pseudodesulfovibrio cashew SRB007 was isolated and purified from the deep-sea cold seep and proposed to represent a novel species in the genus of Pseudodesulfovibrio. A detailed description of the phenotypic traits, phylogenetic status and central metabolisms of strain SRB007 allowed the reconstruction of the metabolic potential and lifestyle of a novel member of deep-sea SRB. Notably, P. cashew SRB007 showed a strong ability to resist and remove different heavy metal ions including Co2+, Ni2+, Cd2+ and Hg2+. The dissimilatory sulfate reduction was demonstrated to contribute to the prominent removal capability of P. cashew SRB007 against different heavy metals via the formation of insoluble metal sulfides.

scholarly journals Dissimilatory sulfate reduction in the archaeon ‘Candidatus Vulcanisaeta moutnovskia’ sheds light on the evolution of sulfur metabolism

2020 ◽  
Vol 5 (11) ◽  
pp. 1428-1438
Author(s):  
Nikolay A. Chernyh ◽  
Sinje Neukirchen ◽  
Evgenii N. Frolov ◽  
Filipa L. Sousa ◽  
Margarita L. Miroshnichenko ◽  
...  
Keyword(s):  
Sulfate Reduction ◽  
Sulfur Metabolism ◽  
Dissimilatory Sulfate Reduction

Generation of zero valent sulfur from dissimilatory sulfate reduction under methanogenic conditions

2020 ◽  
Vol 383 ◽  
pp. 121197
Author(s):  
Wenwen Fang ◽  
Manfei Gu ◽  
Dongqing Liang ◽  
Guang-Hao Chen ◽  
Shanquan Wang
Keyword(s):  
Sulfate Reduction ◽  
Dissimilatory Sulfate Reduction

scholarly journals Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

2020 ◽  
Vol 9 (6) ◽  
pp. 1920 ◽  
Author(s):  
Ivan Kushkevych ◽  
Jorge Castro Sangrador ◽  
Dani Dordević ◽  
Monika Rozehnalová ◽  
Martin Černý ◽  
...  
Keyword(s):  
Sulfate Reduction ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Healthy Individuals ◽  
Fecal Samples ◽  
Sulfate Reducing ◽  
Cell Extracts ◽  
Production Of Hydrogen ◽  
Reducing Bacteria

Background: Inflammatory bowel diseases (IBDs) are multifactorial illnesses of the intestine, to which microorganisms are contributing. Among the contributing microorganisms, sulfate-reducing bacteria (SRB) are suggested to be involved in the process of bowel inflammation due to the production of hydrogen sulfide (H2S) by dissimilatory sulfate reduction. The aims of our research were to physiologically examine SRB in fecal samples of patients with IBD and a control group, their identification, the study of the process of dissimilatory sulfate reduction (sulfate consumption and H2S production) and biomass accumulation. Determination of biogenic elements of the SRB and evaluation of obtained parameters by using statistical methods were also included in the research. The material for the research consisted of 14 fecal samples, which was obtained from patients and control subjects. Methods: Microscopic techniques, microbiological, biochemical, biophysical methods and statistical analysis were included. Results: Colonies of SRB were isolated from all the fecal samples, and subsequently, 35 strains were obtained. Vibrio-shaped cells stained Gram-negative were dominant in all purified studied strains. All strains had a high percentage of similarity by the 16S rRNA gene with deposited sequences in GenBank of Desulfovibrio vulgaris. Cluster analysis of sulfate reduction parameters allowed the grouping of SRB strains. Significant (p < 0.05) differences were not observed between healthy individuals and patients with IBD with regard to sulfate reduction parameters (sulfate consumption, H2S and biomass accumulation). Moreover, we found that manganese and iron contents in the cell extracts are higher among healthy individuals in comparison to unhealthy individuals that have an intestinal bowel disease, especially ulcerative colitis. Conclusions: The observations obtained from studying SRB emphasize differences in the intestinal microbial processes of healthy and unhealthy people.

Metagenomic insights into production of zero valent sulfur from dissimilatory sulfate reduction in a methanogenic bioreactor

2019 ◽  
Vol 8 ◽  
pp. 100305
Author(s):  
Wenwen Fang ◽  
Zhiwei Liang ◽  
Yulong Liu ◽  
Jiayuan Liao ◽  
Shanquan Wang
Keyword(s):  
Sulfate Reduction ◽  
Dissimilatory Sulfate Reduction

scholarly journals Diversity of Sulfur Isotope Fractionations by Sulfate-Reducing Prokaryotes

2001 ◽  
Vol 67 (2) ◽  
pp. 888-894 ◽  
Author(s):  
Jan Detmers ◽  
Volker Brüchert ◽  
Kirsten S. Habicht ◽  
Jan Kuever
Keyword(s):  
Sulfate Reduction ◽  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Reduction Rate ◽  
Sulfate Reduction Rate ◽  
Dissimilatory Sulfate Reduction ◽  
Systematic Effect ◽  
Sulfate Reducers ◽  
Sulfate Reducing ◽  
Sulfur Isotope Fractionation

ABSTRACT Batch culture experiments were performed with 32 different sulfate-reducing prokaryotes to explore the diversity in sulfur isotope fractionation during dissimilatory sulfate reduction by pure cultures. The selected strains reflect the phylogenetic and physiologic diversity of presently known sulfate reducers and cover a broad range of natural marine and freshwater habitats. Experimental conditions were designed to achieve optimum growth conditions with respect to electron donors, salinity, temperature, and pH. Under these optimized conditions, experimental fractionation factors ranged from 2.0 to 42.0‰. Salinity, incubation temperature, pH, and phylogeny had no systematic effect on the sulfur isotope fractionation. There was no correlation between isotope fractionation and sulfate reduction rate. The type of dissimilatory bisulfite reductase also had no effect on fractionation. Sulfate reducers that oxidized the carbon source completely to CO2 showed greater fractionations than sulfate reducers that released acetate as the final product of carbon oxidation. Different metabolic pathways and variable regulation of sulfate transport across the cell membrane all potentially affect isotope fractionation. Previous models that explained fractionation only in terms of sulfate reduction rates appear to be oversimplified. The species-specific physiology of each sulfate reducer thus needs to be taken into account to understand the regulation of sulfur isotope fractionation during dissimilatory sulfate reduction.

scholarly journals Disguised as a Sulfate Reducer: Growth of the Deltaproteobacterium Desulfurivibrio alkaliphilus by Sulfide Oxidation with Nitrate

mBio ◽  
2017 ◽  
Vol 8 (4) ◽  
Author(s):  
Casper Thorup ◽  
Andreas Schramm ◽  
Alyssa J. Findlay ◽  
Kai W. Finster ◽  
Lars Schreiber
Keyword(s):  
Sulfate Reduction ◽  
Elemental Sulfur ◽  
Sulfide Oxidation ◽  
Model Organism ◽  
Sulfur Cycle ◽  
High Expression ◽  
Sulfate Reducer ◽  
Expression Of Genes ◽  
Complex Picture ◽  
Pure Cultures

ABSTRACT This study demonstrates that the deltaproteobacterium Desulfurivibrio alkaliphilus can grow chemolithotrophically by coupling sulfide oxidation to the dissimilatory reduction of nitrate and nitrite to ammonium. Key genes of known sulfide oxidation pathways are absent from the genome of D. alkaliphilus. Instead, the genome contains all of the genes necessary for sulfate reduction, including a gene for a reductive-type dissimilatory bisulfite reductase (DSR). Despite this, growth by sulfate reduction was not observed. Transcriptomic analysis revealed a very high expression level of sulfate-reduction genes during growth by sulfide oxidation, while inhibition experiments with molybdate pointed to elemental sulfur/polysulfides as intermediates. Consequently, we propose that D. alkaliphilus initially oxidizes sulfide to elemental sulfur, which is then either disproportionated, or oxidized by a reversal of the sulfate reduction pathway. This is the first study providing evidence that a reductive-type DSR is involved in a sulfide oxidation pathway. Transcriptome sequencing further suggests that nitrate reduction to ammonium is performed by a novel type of periplasmic nitrate reductase and an unusual membrane-anchored nitrite reductase. IMPORTANCE Sulfide oxidation and sulfate reduction, the two major branches of the sulfur cycle, are usually ascribed to distinct sets of microbes with distinct diagnostic genes. Here we show a more complex picture, as D. alkaliphilus, with the genomic setup of a sulfate reducer, grows by sulfide oxidation. The high expression of genes typically involved in the sulfate reduction pathway suggests that these genes, including the reductive-type dissimilatory bisulfite reductases, are also involved in as-yet-unresolved sulfide oxidation pathways. Finally, D. alkaliphilus is closely related to cable bacteria, which grow by electrogenic sulfide oxidation. Since there are no pure cultures of cable bacteria, D. alkaliphilus may represent an exciting model organism in which to study the physiology of this process. IMPORTANCE Sulfide oxidation and sulfate reduction, the two major branches of the sulfur cycle, are usually ascribed to distinct sets of microbes with distinct diagnostic genes. Here we show a more complex picture, as D. alkaliphilus, with the genomic setup of a sulfate reducer, grows by sulfide oxidation. The high expression of genes typically involved in the sulfate reduction pathway suggests that these genes, including the reductive-type dissimilatory bisulfite reductases, are also involved in as-yet-unresolved sulfide oxidation pathways. Finally, D. alkaliphilus is closely related to cable bacteria, which grow by electrogenic sulfide oxidation. Since there are no pure cultures of cable bacteria, D. alkaliphilus may represent an exciting model organism in which to study the physiology of this process.

Kinetic oxygen isotope effects during dissimilatory sulfate reduction: A combined theoretical and experimental approach

2010 ◽  
Vol 74 (7) ◽  
pp. 2011-2024 ◽  
Author(s):  
Alexandra V. Turchyn ◽  
Volker Brüchert ◽  
Timothy W. Lyons ◽  
Gregory S. Engel ◽  
Nurgul Balci ◽  
...  
Keyword(s):  
Sulfate Reduction ◽  
Oxygen Isotope ◽  
Experimental Approach ◽  
Isotope Effects ◽  
Dissimilatory Sulfate Reduction
Sign in / Sign up

Export Citation Format

Share Document