scholarly journals Evaluation of the effects of the bonding agent on acid-etched human enamel demineralization: in situ study

2012 ◽  
Vol 35 (3) ◽  
pp. 369-374 ◽  
Author(s):  
M. Tostes ◽  
J. N. Mucha ◽  
T. C. Lopes Coutinho ◽  
E. M. da Silva
Keyword(s):  
Bonding Agent ◽  
In Situ Study ◽  
Enamel Demineralization ◽  
Human Enamel

Effect of Dentifrice Containing Fluoride and/or Baking Soda on Enamel Demineralization/ Remineralization: An in situ Study

2001 ◽  
Vol 35 (2) ◽  
pp. 106-110 ◽  
Author(s):  
J.A. Cury ◽  
L.N. Hashizume ◽  
A.A. Del Bel Cury ◽  
C.P.M. Tabchoury
Keyword(s):  
In Situ Study ◽  
Enamel Demineralization

In situ Mineral Loss Inhibition by CO2 Laser and Fluoride

2006 ◽  
Vol 85 (7) ◽  
pp. 617-621 ◽  
Author(s):  
L.K.A. Rodrigues ◽  
M. Nobre dos Santos ◽  
J.D.B. Featherstone
Keyword(s):  
Laser Irradiation ◽  
Laser Treatment ◽  
Co2 Laser ◽  
Sucrose Solution ◽  
Treatment Groups ◽  
Enamel Demineralization ◽  
Human Enamel ◽  
Fluoride Dentifrice ◽  
Mineral Loss

Laser and fluoride treatments have been shown to inhibit enamel demineralization in the laboratory. However, the intra-oral effects of this association have not been tested. This study assessed in situ the effect of a Transversely Excited Atmospheric CO2 laser (λ = 9.6 μm) and the use of pressure fluoridated dentifrice on enamel demineralization. During two 14-day phases, 17 volunteers wore palatal appliances containing human enamel slabs assigned to treatment groups, as follows: (1) non-fluoride dentifrice, (2) CO2 laser irradiation plus non-fluoride dentifrice, (3) fluoride dentifrice, and (4) CO2 laser irradiation plus fluoride dentifrice. A 20% sucrose solution was dripped onto the slabs 8 times per day. The specimens treated with laser and/or fluoridated dentifrice presented a significantly lower mineral loss when compared with those from the non-fluoride dentifrice group. The results suggested that CO2 laser treatment of enamel inhibits demineralization in the human mouth, being more effective when associated with fluoride.

In Situ Study of In-office Bleaching Procedures Using Light Sources on Human Enamel Microhardness

2010 ◽  
Vol 35 (2) ◽  
pp. 139-146 ◽  
Author(s):  
F. O. Araujo ◽  
L. N. Baratieri ◽  
É Araújo
Keyword(s):  
Clinical Relevance ◽  
Dental Enamel ◽  
Light Sources ◽  
In Situ Study ◽  
Human Enamel

Clinical Relevance Regardless of the light sources used, the microhardness of human dental enamel did not present significant changes 14 days after in-office bleaching.

Frequency of Application of AmF/NaF/SnCl2 Solution and Its Potential in Controlling Human Enamel Erosion Progression: An in situ Study

2017 ◽  
Vol 51 (2) ◽  
pp. 141-148 ◽  
Author(s):  
C.V. da Silva ◽  
T.M. Ramos-Oliveira ◽  
T.F. Mantilla ◽  
P.M. de Freitas
Keyword(s):  
Citric Acid ◽  
Morphological Changes ◽  
Treatment Protocol ◽  
Negative Control ◽  
Tissue Loss ◽  
In Situ Study ◽  
Acid Ph ◽  
Human Enamel

Although several studies have demonstrated the efficacy of AmF/NaF/SnCl2 solution in inhibiting dental erosion progression, measures for further improvement in its effectiveness are paramount. Thus, this in situ study evaluated whether the protective effect promoted by the AmF/NaF/SnCl2 solution would be enhanced by increasing its frequency of use. The study was conducted with 12 volunteers, a 4-phase (5 days each) randomized, crossover model. Extraoral erosive challenges (0.5% citric acid, pH 2.6, 6 × 2 min/day) and rinsing protocol (1 or 2 × 2 min/day) were performed. Before the in situ phase, human enamel samples were subjected to an in vitro surface softening (1% citric acid, pH 4.0, for 3 min). Four treatment protocols were tested using samples in replicas (n = 12): group G1 - deionized water (negative control); G2 - NaF solution (positive control, 500 ppm F-, pH 4.5); G3 - AmF/NaF/SnCl2 solution (500 ppm F-, 800 ppm Sn2+, pH 4.5) once a day; G4 - AmF/NaF/SnCl2 solution twice a day. Tissue loss and morphological changes were determined by optical profilometry (n = 12) and scanning electron microscopy (n = 3) analysis, respectively. Data were statistically analyzed by ANOVA with subsequent pairwise comparison of treatments. Tissue loss means (±SD in µm) for each treatment protocol and statistical differences were found as follows: G1 4.55 ± 2.75, G2 4.59 ± 2.13, G3 2.64 ± 1.55, and G4 1.34 ± 1.16. Although there was no difference between the 2 AmF/NaF/SnCl2 solution application regimens (once or twice a day), application of the product twice a day was the only treatment that was able to control erosion progression, differing from the control groups.

The Effect of Sucrose Application and Implantation of Mutans Streptococci on the Microbial Composition of Three-week Experimental Plaque—An in situ Study

1990 ◽  
Vol 69 (11) ◽  
pp. 1706-1711 ◽  
Author(s):  
L.M.D. Macpherson ◽  
T.W. MacFarlane ◽  
T.C. Aitchison ◽  
K.W. Stephen
Keyword(s):  
Mutans Streptococci ◽  
Microbial Composition ◽  
Microbial Identification ◽  
Isolation Frequency ◽  
In Situ Study ◽  
Human Enamel ◽  
Experimental Protocols ◽  
Oral Sucrose ◽  
Removable Appliances

This study describes the predominant cultivable microflora of three-week-old plaque samples obtained from human enamel sites, on the basis of microbial identification of over 9000 fresh isolates. Lower removable appliances, on which were mounted enamel sections and slabs, were worn by five young adult subjects under three experimental protocols. These were (1) 'normal' plaque conditions, (2) extra-oral sucrose applications nine times daily, and (3) inoculation of each subject's own mutans streptococci onto the enamel test sites and sucrose applications, as described above. With the exception of slightly higher proportions of Gram-negative bacilli associated with slab plaque following sucrose application, no significant differences in percentage or absolute counts of organisms were found between normal and sucrose plaques. The inoculation of mutans streptococci, combined with extra-oral sucrose applications, was associated with significantly higher percentages and absolute mean counts of both mutans streptococci and lactobacilli, and lower proportions of S. sanguis and S. oralis. Although the isolation frequency of mutans streptococci increased in all subjects and the overall mean proportion rose following inoculation, considerable inter-subject variation was seen in mean percentage counts of these organisms isolated from the three-week plaque samples.

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