Evaluation of Reference Genes for RT-qPCR in Tribolium castaneum (Coleoptera: Tenebrionidae) Under UVB Stress

2015 ◽  
Vol 44 (2) ◽  
pp. 418-425 ◽  
Author(s):  
W. Sang ◽  
L. He ◽  
X.-P. Wang ◽  
K. Zhu-Salzman ◽  
C.-L. Lei
Keyword(s):  
Tribolium Castaneum ◽  
Reference Genes ◽  
Coleoptera Tenebrionidae

Efficacy of some indigenous plants and a synthetic insecticide for the control of Tribolium castaneum (herbst.) (coleoptera: tenebrionidae)

2016 ◽  
Vol 5 (01) ◽  
pp. 4742
Author(s):  
Mumtaz Akhtar ◽  
Naheed Akhtar* ◽  
Muhammad Arshad ◽  
Naima Iram ◽  
Muhammad Iqbal ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Eucalyptus Camaldulensis ◽  
Ethanol Extract ◽  
Cumulative Effects ◽  
Piper Nigrum ◽  
Paper Test ◽  
Indigenous Plants ◽  
Synthetic Insecticide ◽  
Insecticidal Action ◽  
Coleoptera Tenebrionidae

Research studies were carried out to evaluate insecticidal action of two plant products and a synthetic insecticide on a major stored-product insect, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). The plant species studied were, Piper nigrum and Eucalyptus camaldulensis, whereas methoprene used as synthetic insecticide. Two formulations viz., powder and ethanol extract of each plant and four concentrations (5ppm, 10ppm, 15ppm and 20 ppm) of methoprene were tested. Repellency was tested using the filter paper test whereas mortality, weight loss protection and anti-feedant potential of all treatments was evaluated by using whole maize grains. Our results were highly significant as compared to control however; P. nigrum was more effective as compared to E. camaldulensis. The investigation of cumulative effects of botanical and synthetic insecticide and isolation of active ingredients of botanicals are further issues to be investigated for their inclusion in. I. P. M programs.

SEX AND CROSSING OVER IN LINKAGE GROUP III OF TRIBOLIUM CASTANEUM

1977 ◽  
Vol 19 (2) ◽  
pp. 259-263 ◽  
Author(s):  
Alexander Sokoloff
Keyword(s):  
Sex Differences ◽  
Linkage Group ◽  
Tribolium Castaneum ◽  
Relative Position ◽  
Genetic Background ◽  
Crossing Over ◽  
Group Iii ◽  
The Third ◽  
Main Factors ◽  
Coleoptera Tenebrionidae

The relative position of the genes black (b), light ocular diaphragm (lod) and aureate (au) for the third linkage group of T. castaneum (Herbst) (Coleoptera, Tenebrionidae) has been determined as b – lod – au. The distances between the various genes vary, depending on the cross. The b++/+ lod au ♂ × + lod au/+ lod au ♀ crosses give the following recombination values: au – lod = 18.32 ± 1.21%; b – lod = 21.05 ± 1.51% and b – au = 37.43 ± 1.27%. The reciprocal crosses give au – lod = 27.67 ± 1.62%; b – lod = 13.97 ± 1.26% and b – au = 39.79 ± 1.78%. For the larger distances encompassed in the b – au region the recombination values in the two sexes were not significantly different. For the shorter b – lod region the recombination values were significantly larger in the females than in the males, while for the adjacent lod – au region the opposite was true. On the basis of the current literature it would appear that the main factors contributing to these sex differences in recombination are the modifiers which are different in the genetic background of the two sexes.

The complete mitochondrial genome of the red flour beetle,Tribolium castaneum(Coleoptera: Tenebrionidae)

2014 ◽  
Vol 27 (2) ◽  
pp. 1525-1527 ◽  
Author(s):  
Qiu-Ning Liu ◽  
Dan-Dan Bian ◽  
Sen-Hao Jiang ◽  
Zhen-Xing Li ◽  
Bao-Ming Ge ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Tribolium Castaneum ◽  
Complete Mitochondrial Genome ◽  
Red Flour Beetle ◽  
Flour Beetle ◽  
Coleoptera Tenebrionidae

Knockdown of Uridine Diphosphate Glucosyltransferase 86Dg Enhances Susceptibility of Tribolium castaneum (Coleoptera: Tenebrionidae) to Artemisia vulgaris (Asterales: Asteraceae) Essential Oil

2021 ◽  
Author(s):  
Shanshan Gao ◽  
Haidi Sun ◽  
Jiahao Zhang ◽  
Yonglei Zhang ◽  
Peipei Sun ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tribolium Castaneum ◽  
Expression Profile ◽  
Fat Body ◽  
Detoxification Enzymes ◽  
Developmental Expression ◽  
Contact Toxicity ◽  
Uridine Diphosphate ◽  
Artemisia Vulgaris ◽  
Coleoptera Tenebrionidae

Abstract Uridine diphosphate glucosyltransferases (UGTs), which are phase II detoxification enzymes, are found in various organisms. These enzymes play an important role in the detoxification mechanisms of plant allelopathy and in insects. Artemisia vulgaris L. (Asterales: Asteraceae: Artemisia) essential oil has strong contact toxicity to Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) larvae. However, the effect of A. vulgaris essential oil on UGTs is unclear. In this study, A. vulgaris essential oil was shown to significantly induce the expression of the TcUgt86Dg transcript. Furthermore, treatment of TcUgt86Dg-silenced individuals with A. vulgaris essential oil resulted in higher mortality than for the control individuals, indicating that TcUgt86Dg is involved in detoxification of A. vulgaris essential oil in T. castaneum. The developmental expression profile showed that the expression of TcUgt86Dg in late adults was higher than in other developmental stages. Furthermore, the expression profile in adult tissues revealed higher expression of TcUgt86Dg in the head, antenna, fat body, and accessory gland than in other tissues. These data show that TcUgt86Dg may be involved in the metabolism of exogenous toxins by T. castaneum; thus, our results have elucidated one possible mechanism of resistance to A. vulgaris essential oil and provide a theoretical basis for a control scheme for T. castaneum.

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