Insect Bioassay for Determining Soil Degradation of Bacillus thuringiensis subsp. kurstaki CryIA(b) Protein in Corn Tissue

1996 ◽  
Vol 25 (3) ◽  
pp. 659-664 ◽  
Author(s):  
Steven R. Sims ◽  
Larry R. Holden
Keyword(s):  
Bacillus Thuringiensis ◽  
Soil Degradation ◽  
Insect Bioassay ◽  
Bacillus Thuringiensis Subsp

Self-transfer and mobilisation capabilities of the pXO2-like plasmid pBT9727 from Bacillus thuringiensis subsp. konkukian 97-27

Plasmid ◽  
2008 ◽  
Vol 59 (2) ◽  
pp. 134-138 ◽  
Author(s):  
Géraldine A. Van der Auwera ◽  
Sophie Timmery ◽  
Jacques Mahillon
Keyword(s):  
Bacillus Thuringiensis ◽  
Bacillus Thuringiensis Subsp

Cytolytic Peptide Fragments of Cyt1Aa from Bacillus thuringiensis subsp. israelensis

2012 ◽  
Vol 65 (2) ◽  
pp. 121-127
Author(s):  
Marina Nisnevitch ◽  
Svetlana Nikonov ◽  
Yeshayahu Nitzan
Keyword(s):  
Bacillus Thuringiensis ◽  
Peptide Fragments ◽  
Bacillus Thuringiensis Subsp

scholarly journals Amino sugars in the glycoprotein toxin from Bacillus thuringiensis subsp. israelensis.

1987 ◽  
Vol 169 (2) ◽  
pp. 796-801 ◽  
Author(s):  
M A Pfannenstiel ◽  
G Muthukumar ◽  
G A Couche ◽  
K W Nickerson
Keyword(s):  
Bacillus Thuringiensis ◽  
Amino Sugars ◽  
Bacillus Thuringiensis Subsp

Cloning of Two New cry Genes from Bacillus thuringiensis subsp. wuhanensis Strain

2000 ◽  
Vol 40 (4) ◽  
pp. 227-232 ◽  
Author(s):  
White-Shang Kuo ◽  
Jong-Huon Lin ◽  
Ching-Chou Tzeng ◽  
Shui-Shang Kao ◽  
Kin-Fu Chak
Keyword(s):  
Bacillus Thuringiensis ◽  
Cry Genes ◽  
Bacillus Thuringiensis Subsp

Purification and characterization of an exochitinase from Bacillus thuringiensis subsp. aizawai and its action against phytopathogenic fungi

2006 ◽  
Vol 52 (7) ◽  
pp. 651-657 ◽  
Author(s):  
Luis Morales de la Vega ◽  
J Eleazar Barboza-Corona ◽  
Maria G Aguilar-Uscanga ◽  
Mario Ramírez-Lepe
Keyword(s):  
Bacillus Thuringiensis ◽  
Sodium Dodecyl ◽  
Sclerotium Rolfsii ◽  
Phytopathogenic Fungi ◽  
Purification And Characterization ◽  
Chitinolytic Enzyme ◽  
Bacillus Thuringiensis Subsp ◽  
Sds Page ◽  
Fusarium Sp

A chitinolytic enzyme from Bacillus thuringiensis subsp. aizawai has been purified and its molecular mass was estimated ca. 66 kDa by sodium dodecyl sulfate – polyacryamide gel electrophoresis (SDS–PAGE). The enzyme was able to hydrolyze chitin to chitobiosides but not carboxymethylcellulose, cellulose, pullulan, and laminarin. Optimal pH and temperature were detected at 6 and 50 °C, respectively. Stability, in the absence of substrate, was observed at temperatures less than 60 °C and pH between 5 and 8. Enzyme activity was significantly inhibited by K+ and EDTA and completely inhibited by Hg2+. Purified chitinase showed lytic activity against cell walls from six phytopathogenic fungi and inhibited the mycelial growth of both Fusarium sp. and Sclerotium rolfsii. The biocontrol efficacy of the enzyme was tested in the protection of bean seeds infested with six phytopathogenic fungi.Key words: chitinase, Bacillus thuringiensis, purification, phytopathogenic fungi.

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