Cloning of Two New cry Genes from Bacillus thuringiensis subsp. wuhanensis Strain

2000 ◽  
Vol 40 (4) ◽  
pp. 227-232 ◽  
Author(s):  
White-Shang Kuo ◽  
Jong-Huon Lin ◽  
Ching-Chou Tzeng ◽  
Shui-Shang Kao ◽  
Kin-Fu Chak
Keyword(s):  
Bacillus Thuringiensis ◽  
Cry Genes ◽  
Bacillus Thuringiensis Subsp

Expression of a Bacillus thuringiensis δ-endotoxin gene by Bacillus pumilus

1998 ◽  
Vol 44 (3) ◽  
pp. 259-269 ◽  
Author(s):  
L B Selinger ◽  
G G Khachatourians ◽  
J R Byers ◽  
M F Hynes
Keyword(s):  
Bacillus Thuringiensis ◽  
Copy Number ◽  
Bacillus Pumilus ◽  
High Copy Number ◽  
Cry Genes ◽  
Bacillus Thuringiensis Subsp ◽  
High Copy Number Plasmid ◽  
Low Copy Number ◽  
High Level ◽  
Lepidoptera Noctuidae

The δ -endotoxin genes from Bacillus thuringiensis were introduced into a rhizosphere-inhabiting Bacillus pumilus isolate to create a δ -endotoxin expression and delivery system for subterranean feeding insects such as the larvae of pale western cutworm (Agrotis orthogonia Morrison (Lepidoptera: Noctuidae)). Preliminary experiments indicated that Bacillus thuringiensis subsp. kurstaki cultures were toxic to pale western cutworm larvae. Three different cry genes from Bacillus thuringiensis subsp. kurstaki were cloned into high and low copy number vectors and mated into Bacillus pumilus RB8. When carried on high copy number vectors, cry genes appeared to inhibit sporulation and δ -endotoxin production in Bacillus pumilus RB8 cultures, since microscopic examination of these cultures revealed that <0.1% of the cells of late stationary phase cultures had sporulated and produced parasporal inclusions. On low copy number vectors, the cry genes did not inhibit sporulation; however, production of δ -endotoxins was undetectable. Using a heat shock regime for enrichment of sporogenous crystalliferous variants, a Bacillus pumilus isolate, carrying cryIA(c) on a high copy number plasmid, was obtained in which high level δ -endotoxin production occurred concomitant with sporulation. Synthesis of functional δ -endotoxin by this strain was confirmed by Western blot analysis and bioassay with pale western cutworm larvae. These results show that rhizosphere-inhabiting bacilli are indeed a potential route for introduction of δ -endotoxins to the root environment for biocontrol purposes.Key words: Bacillus thuringiensis, δ -endotoxin, conjugation, sporulation, expression.

Self-transfer and mobilisation capabilities of the pXO2-like plasmid pBT9727 from Bacillus thuringiensis subsp. konkukian 97-27

Plasmid ◽  
2008 ◽  
Vol 59 (2) ◽  
pp. 134-138 ◽  
Author(s):  
Géraldine A. Van der Auwera ◽  
Sophie Timmery ◽  
Jacques Mahillon
Keyword(s):  
Bacillus Thuringiensis ◽  
Bacillus Thuringiensis Subsp

Diversity analysis and characterization of Coleoptera-, Hemiptera- and Nematode-active cry genes in native isolates of Bacillus thuringiensis

2013 ◽  
Vol 64 (1) ◽  
pp. 85-98 ◽  
Author(s):  
Ramasamy Asokan ◽  
Hanchipura Mallesh Mahadeva Swamy ◽  
Geetha G. Thimmegowda ◽  
Riaz Mahmood
Keyword(s):  
Bacillus Thuringiensis ◽  
Diversity Analysis ◽  
Cry Genes

Cytolytic Peptide Fragments of Cyt1Aa from Bacillus thuringiensis subsp. israelensis

2012 ◽  
Vol 65 (2) ◽  
pp. 121-127
Author(s):  
Marina Nisnevitch ◽  
Svetlana Nikonov ◽  
Yeshayahu Nitzan
Keyword(s):  
Bacillus Thuringiensis ◽  
Peptide Fragments ◽  
Bacillus Thuringiensis Subsp

scholarly journals Amino sugars in the glycoprotein toxin from Bacillus thuringiensis subsp. israelensis.

1987 ◽  
Vol 169 (2) ◽  
pp. 796-801 ◽  
Author(s):  
M A Pfannenstiel ◽  
G Muthukumar ◽  
G A Couche ◽  
K W Nickerson
Keyword(s):  
Bacillus Thuringiensis ◽  
Amino Sugars ◽  
Bacillus Thuringiensis Subsp
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