scholarly journals RSC96 Schwann Cell Proliferation and Survival Induced by Dilong through PI3K/Akt Signaling Mediated by IGF-I

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Yung-Ming Chang ◽  
Wu-Hsien Kuo ◽  
Tung-Yuan Lai ◽  
Ying-Ting Shih ◽  
Fuu-Jen Tsai ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Herbal Medicine ◽  
Schwann Cell ◽  
Protein Expression ◽  
Chinese Herbal Medicine ◽  
Time Dependent ◽  
Nuclear Antigen ◽  
Dependent Manner ◽  
Chinese Herbal ◽  
Igf I

Schwann cell proliferation is critical for the regeneration of injured nerves. Dilongs are widely used in Chinese herbal medicine to remove stasis and stimulate wound-healing functions. Exactly how this Chinese herbal medicine promotes tissue survival remains unclear. The aim of the present study was to investigate the molecular mechanisms by which Dilong promote neuron regeneration. Our results show that treatment with extract of Dilong induces the phosphorylation of the insulin-like growth factor-I (IGF-I)-mediated phosphatidylinositol 3-kinase/serine-threonine kinase (PI3K/Akt) pathway, and activates protein expression of cell nuclear antigen (PCNA) in a time-dependent manner. Cell cycle analysis showed that G1transits into the S phase in 12–16 h, and S transits into the G2phase 20 h after exposure to earthworm extract. Strong expression of cyclin D1, cyclin E and cyclin A occurs in a time-dependent manner. Small interfering RNA (siRNA)-mediated knockdown of PI3K significantly reduced PI3K protein expression levels, resulting in Bcl2survival factor reduction and a marked blockage of G1to S transition in proliferating cells. These results demonstrate that Dilong promotes the proliferation and survival of RSC96 cells via IGF-I signaling. The mechanism is mainly dependent on the PI3K protein.

DHT and testosterone, but not DHEA or E2, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells

2006 ◽  
Vol 290 (5) ◽  
pp. E952-E960 ◽  
Author(s):  
Hanh Le ◽  
Julia T. Arnold ◽  
Kimberly K. McFann ◽  
Marc R. Blackman
Keyword(s):  
Prostate Cancer ◽  
Cell Proliferation ◽  
Mrna Expression ◽  
Protein Secretion ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Time Dependent ◽  
Dependent Manner ◽  
Igf I ◽  
Igfbp 3

Prostate cancer is one of the four most common cancers in the United States, affecting one of six men. Increased serum levels of androgens and IGF-I are associated with an augmented risk of prostate cancer. Dihydrotestosterone (DHT) and testosterone (T) stimulate prostate cancer cell growth, development, and function, whereas the effects of DHT and T in prostate stromal cells, and of dehydroepiandrosterone (DHEA) in prostate cancer or stromal cells, are uncertain. We investigated the actions of DHT, T, DHEA, and estradiol (E2) on insulin-like growth factor (IGF)-I, IGF-II, IGF-I receptor (R), IGF-binding protein (IGFBP)-2, IGFBP-3, and IGFBP-5 in primary cultures of human prostatic stromal cells by assessing cell proliferation, mRNA expression, and protein secretion by MTT growth assay, quantitative real-time PCR, and ELISA, respectively. DHT and T each increased IGF-I (7-fold) and decreased IGFBP-3 (2-fold) mRNA expression and protein secretion in a dose- and time-dependent manner and increased IGFBP-2 (2-fold) mRNA in a dose- and time-dependent manner. DHEA and E2did not significantly alter these measures. Flutamide abolished the DHT-modulated increases in IGF-I and IGFBP-2, suggesting that the influences of DHT and T on these measures were androgen receptor mediated. None of the four steroids significantly affected IGF-IR, IGF-II, or IGFBP-5 mRNA levels or stromal cell proliferation. The effects of DHT on IGF-I, IGFBP-2, and IGFBP-3 were more pronounced in stromal cultures that did not express desmin. These data suggest that DHT and T promote prostate growth partly via modulation of the stromal cell IGF axis, with potential paracrine effects on prostate epithelial cells.

scholarly journals Effects ofAngelicaExtract on Schwann Cell Proliferation and Expressions of Related Proteins

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaowen Jiang ◽  
Lin Liu ◽  
Binqing Zhang ◽  
Ziyin Lu ◽  
Lu Qiao ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Schwann Cell ◽  
Nuclear Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Dependent Manner ◽  
Cell Cycles ◽  
Neural Cell Adhesion ◽  
Related Proteins ◽  
Proliferating Cell

The present study investigated the effects ofAngelicaextract (AE) on Schwann cell proliferation and expressions of related proteins, including brain derived neurotrophic factor (BDNF), neural cell adhesion molecule (NCAM), and proliferating cell nuclear antigen (PCNA). Proliferation activity and cell cycles of SCs were evaluated by MTT assay and flow cytometry methods, respectively, after 12 h treatment of AE at different concentrations (62.5, 125, 250, 1000, 2000, 4000, and 8000 mg/L). SCs were treated by 500, 1000, and 2000 mg/L AE for 24 h or 48 h; the related genes mRNA and proteins expressions in SCs were detected by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) kit. At the concentration range of 125–2000 mg/L, the SC proliferation was induced by AE in a dose-dependent manner, especially 1000 and 2000 mg/L; cells in drug-treated groups showed the most increase.Cells counts were ascended significantly in (G2/M + S) phase compared to control group. BDNF, NCAM, and PCNA protein expressions significantly increased at drug-treated groups. Relative genes mRNA expressions levels were also significantly higher compared to control group. The results indicated that AE facilitated SC proliferation and related genes and proteins expressions, which provided a basic guideline for nerve injury repair in clinic.

scholarly journals Chinese Herbal Medicine Fuzheng Kang-Ai Decoction Inhibited Lung Cancer Cell Growth through AMPKα-Mediated Induction and Interplay of IGFBP1 and FOXO3a

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Fang Zheng ◽  
Jingjing Wu ◽  
Xiong Li ◽  
Qing Tang ◽  
LiJun Yang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Growth ◽  
Herbal Medicine ◽  
Protein Expression ◽  
Chinese Herbal Medicine ◽  
Specific Inhibitor ◽  
Mice Model ◽  
Chinese Herbal ◽  
Compound C ◽  
Exogenous Expression

The aim of this study is to investigate the actions of Chinese herbal medicine, called “Fuzheng Kang-Ai” (FZKA for short) decoction, against non-small cell lung cancer (NSCLC) and its mechanismsin vitroandin vivo. We showed that the effect of FZKA decoction significantly inhibited growth of A549 and PC9 cells. Furthermore, FZKA increased phosphorylation of AMP-activated protein kinase alpha (AMPKα) and induced protein expression of insulin-like growth factor (IGF) binding protein 1 (IGFBP1) and forkhead homeobox type O3a (FOXO3a). The specific inhibitor of AMPKα(Compound C) blocked FZKA-induced protein expression of IGFBP1 and FOXO3a. Interestingly, silencing of IGFBP1 and FOXO3a overcame the inhibitory effect of FZKA on cell growth. Moreover, silencing of IGFBP1 attenuated the effect of FZKA decoction on FOXO3a expression, and exogenous expression of FOXO3a enhanced the FZKA-stimulated phosphorylation of AMPKα. Accordingly, FZKA inhibited the tumor growth in xenograft nude mice model. Collectively, our results show that FZKA decoction inhibits proliferation of NSCLC cells through activation of AMPKα, followed by induction of IGFBP1 and FOXO3a proteins. Exogenous expression of FOXO3a feedback enhances FZKA decoction-stimulated IGFBP1 expression and phosphorylation of AMPKα. The reciprocal interplay of IGFBP1 and FOXO3a contribute to the overall responses of FAKA decoction.

scholarly journals Antagonistic Effects of Tetramethylpyrazine on Hypoxic Respiratory Depression in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Li Li ◽  
Bo Gao ◽  
Meifang Liu ◽  
Manel Santafe
Keyword(s):  
Herbal Medicine ◽  
Protein Expression ◽  
Respiratory Depression ◽  
Chinese Herbal Medicine ◽  
Protective Effects ◽  
Antagonistic Effects ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Hypoxia Treatment ◽  
Respiratory Responses

Objective. Tetramethylpyrazine (TMP) is an alkaloid extracted from the root and stem of the traditional Chinese herbal medicine called Chuanxiong. The present study aims to study the effects of TMP on hypoxic respiratory depression in rats. Materials and methods. The effects of TMP on respiratory responses of rats induced by hypoxia were observed by diaphragm electromyogram (EMG) recording. The effects of TMP on the protein expression of FOS and acid sensing ion channel1a (ASIC1a) in the brainstem induced by hypoxia were investigated by immunohistochemistry. Results. The respiration of rats was first excited and then depressed during hypoxia treatment, while TMP pretreatment could significantly antagonize the respiratory depression induced by hypoxia P < 0.01 . Hypoxia obviously induced the protein expression of FOS P < 0.01 and ASIC1a P < 0.05 in the brainstem, which can be also significantly inhibited by TMP pretreatment. Conclusions. TMP has protective effects on hypoxic respiratory depression, and the mechanisms might be concerned with its downregulation of FOS and ASIC1a in the brainstem induced by hypoxia.

The effect of the chinese herbal medicine Dai-Kenchu-To on intestinal blood flow

2001 ◽  
Vol 120 (5) ◽  
pp. A248-A248
Author(s):  
N KAWASAKI ◽  
K NARIAI ◽  
M NAKAO ◽  
K NAKADA ◽  
N HANYUU ◽  
...  
Keyword(s):  
Blood Flow ◽  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Intestinal Blood Flow ◽  
Chinese Herbal

Treatment of atopic dermatitis with Chinese herbal medicine. Therapeutic effects of Hochuekkitou.

1989 ◽  
Vol 51 (5) ◽  
pp. 1003-1013 ◽  
Author(s):  
Hiromi KOBAYASHI ◽  
Masamitsu ISHII ◽  
Tsukasa TANII ◽  
Takeshi KOHNO ◽  
Toshio HAMADA
Keyword(s):  
Atopic Dermatitis ◽  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Therapeutic Effects ◽  
Chinese Herbal
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