scholarly journals ctcRbase: the gene expression database of circulating tumor cells and microemboli

Database ◽  
2020 ◽  
Vol 2020 ◽  
Author(s):  
Lei Zhao ◽  
Xiaohong Wu ◽  
Tong Li ◽  
Jian Luo ◽  
Dong Dong
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Metastasis ◽  
Circulating Tumor Cell ◽  
Gene Signature ◽  
Primary Tumors ◽  
A Genome ◽  
Experimental Validations ◽  
Transcriptomic Changes

Abstract Circulating tumor cells/microemboli (CTCs/CTMs) are malignant cells that depart from cancerous lesions and shed into the bloodstream. Analysis of CTCs can allow the investigation of tumor cell biomarker expression from a non-invasive liquid biopsy. To date, high-throughput technologies have become a powerful tool to provide a genome-wide view of transcriptomic changes associated with CTCs/CTMs. These data provided us much information to understand the tumor heterogeneity, and the underlying molecular mechanism of tumor metastases. Unfortunately, these data have been deposited into various repositories, and a uniform resource for the cancer metastasis is still unavailable. To this end, we integrated previously published transcriptome datasets of CTCs/CTMs and constructed a web-accessible database. The first release of ctcRbase contains 526 CTCs/CTM samples across seven cancer types. The expression of 14 631 mRNAs and 3642 long non-coding RNAs of CTCs/CTMs were included. Experimental validations from the published literature are also included. Since CTCs/CTMs are considered to be precursors of metastases, ctcRbase also collected the expression data of primary tumors and metastases, which allows user to discover a unique ‘circulating tumor cell gene signature’ that is distinct from primary tumor and metastases. An easy-to-use database was constructed to query and browse CTCs/CTMs genes. ctcRbase can be freely accessible at http://www.origin-gene.cn/database/ctcRbase/.

Fundamentals of integrated ferrohydrodynamic cell separation in circulating tumor cell isolation

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yang Liu ◽  
Wujun Zhao ◽  
Rui Cheng ◽  
Bryana N. Harris ◽  
Jonathan R. Murrow ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Recovery Rate ◽  
Cell Separation ◽  
Circulating Tumor Cell ◽  
Fundamental Theory ◽  
High Recovery ◽  
High Recovery Rate ◽  
Experimental Validations

We present the fundamental theory and experimental validations of an integrated ferrohydrodynamic cell separation (iFCS) method that can isolate circulating tumor cells with a high recovery rate.

Circulating tumor cell detection using a parallel flow micro-aperture chip system

Lab on a Chip ◽  
2015 ◽  
Vol 15 (7) ◽  
pp. 1677-1688 ◽  
Author(s):  
Chun-Li Chang ◽  
Wanfeng Huang ◽  
Shadia I. Jalal ◽  
Bin-Da Chan ◽  
Aamer Mahmood ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Circulating Tumor Cell ◽  
Parallel Flow ◽  
Cell Detection ◽  
Tumor Cell Detection ◽  
System P

A parallel flow micro-aperture chip system for detection of circulating tumor cells.

scholarly journals Microfluidics-enabled rapid manufacturing of hierarchical silica-magnetic microflower toward enhanced circulating tumor cell screening

2018 ◽  
Vol 6 (12) ◽  
pp. 3121-3125 ◽  
Author(s):  
Nanjing Hao ◽  
Yuan Nie ◽  
Amogha Tadimety ◽  
Ting Shen ◽  
John X. J. Zhang
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Circulating Tumor Cell ◽  
Rapid Manufacturing ◽  
Screening Efficiency

Microfluidics-enabled rapid manufacturing of a hierarchical silica-magnetic microflower was developed for improving the screening efficiency of circulating tumor cells.

scholarly journals Nanotechnology-Assisted Isolation and Analysis of Circulating Tumor Cells on Microfluidic Devices

Micromachines ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 774 ◽  
Author(s):  
Jie Cheng ◽  
Yang Liu ◽  
Yang Zhao ◽  
Lina Zhang ◽  
Lingqian Zhang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Cancer Metastasis ◽  
Human Peripheral Blood ◽  
Microfluidic Devices ◽  
Detection Sensitivity ◽  
Cell Capture ◽  
Primary Tumors ◽  
Downstream Analysis

Circulating tumor cells (CTCs), a type of cancer cell that spreads from primary tumors into human peripheral blood and are considered as a new biomarker of cancer liquid biopsy. It provides the direction for understanding the biology of cancer metastasis and progression. Isolation and analysis of CTCs offer the possibility for early cancer detection and dynamic prognosis monitoring. The extremely low quantity and high heterogeneity of CTCs are the major challenges for the application of CTCs in liquid biopsy. There have been significant research endeavors to develop efficient and reliable approaches to CTC isolation and analysis in the past few decades. With the advancement of microfabrication and nanomaterials, a variety of approaches have now emerged for CTC isolation and analysis on microfluidic platforms combined with nanotechnology. These new approaches show advantages in terms of cell capture efficiency, purity, detection sensitivity and specificity. This review focuses on recent progress in the field of nanotechnology-assisted microfluidics for CTC isolation and detection. Firstly, CTC isolation approaches using nanomaterial-based microfluidic devices are summarized and discussed. The different strategies for CTC release from the devices are specifically outlined. In addition, existing nanotechnology-assisted methods for CTC downstream analysis are summarized. Some perspectives are discussed on the challenges of current methods for CTC studies and promising research directions.

scholarly journals Precision microfilters as an all in one system for multiplex analysis of circulating tumor cells

RSC Advances ◽  
2016 ◽  
Vol 6 (8) ◽  
pp. 6405-6414 ◽  
Author(s):  
Daniel L. Adams ◽  
R. Katherine Alpaugh ◽  
Stuart S. Martin ◽  
Monica Charpentier ◽  
Saranya Chumsri ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Circulating Tumor Cell ◽  
Clinical Testing ◽  
Multiplex Analysis

An all-in-one Circulating Tumor Cell (CTC) isolation platform with streamlined multiplex integration of proteomic and genomic clinical testing.

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