scholarly journals Invasive Disease Due to Haemophilus influenzae Serotype f: Clinical and Epidemiologic Characteristics in the H. influenzae Serotype b Vaccine Era

1996 ◽  
Vol 22 (6) ◽  
pp. 1069-1076 ◽  
Author(s):  
G. Urwin ◽  
J. A. Krohn ◽  
K. D. Robinson ◽  
J. D. Wenger ◽  
M. M. Farley ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Invasive Disease ◽  
Serotype B ◽  
Epidemiologic Characteristics

scholarly journals Fatal, Fulminant and Invasive Non-Typeable Haemophilus influenzae Infection in a Preterm Infant: A Re-Emerging Cause of Neonatal Sepsis

2020 ◽  
Vol 5 (1) ◽  
pp. 30 ◽  
Author(s):  
Sudipta Roy Chowdhury ◽  
Srabani Bharadwaj ◽  
Suresh Chandran
Keyword(s):  
Haemophilus Influenzae ◽  
Preterm Infant ◽  
Neonatal Sepsis ◽  
Group B Streptococcus ◽  
Invasive Disease ◽  
Preventative Measures ◽  
Increased Risk ◽  
Extremely Preterm ◽  
Serotype B ◽  
Group B

Early-onset neonatal sepsis (EOS) is a major cause of neonatal death and long-term neurodevelopmental disabilities among survivors. The common pathogens causing EOS are group B streptococcus (GBS) and Escherichia coli. Haemophilus influenzae (H. influenzae) is a Gram-negative coccobacillus that can cause severe invasive disease and can be divided into either typeable or non-typeable strains. H. influenzae serotype b (Hib) is the most virulent and the major cause of bacterial meningitis in young children prior to routine immunization against Hib. Hib infection rates have dramatically reduced since then. However, a number of studies have reported an increasing incidence of non-typeable H. influenzae (NTHi) sepsis in neonates worldwide and concluded that pregnant women may have an increased risk to invasive NTHi disease with poor pregnancy outcomes. We present a case of fulminant neonatal sepsis caused by NTHi in an extremely preterm infant and discuss potential preventative measures to reduce its re-emergence.

scholarly journals Emergence of Non-Serotype b EncapsulatedHaemophilus influenzaeas a Cause of Pediatric Meningitis in Northwestern Ontario

2013 ◽  
Vol 24 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Pouya Sadeghi-Aval ◽  
Raymond SW Tsang ◽  
Frances B Jamieson ◽  
Marina Ulanova
Keyword(s):  
Bacterial Meningitis ◽  
Haemophilus Influenzae ◽  
Present Article ◽  
Genetic Background ◽  
Antibiotic Sensitivity ◽  
Invasive Disease ◽  
Northwestern Ontario ◽  
Clonal Nature ◽  
Serotype B ◽  
Meningitis In Children

Before the introduction of the conjugate vaccine,Haemophilus influenzaeserotype b (Hib) was the leading cause of bacterial meningitis in children. Although successful in reducing Hib cases, the vaccine confers no protection against other serotypes ofH influenzae, such as a (Hia), or f (Hif). The emergence of invasive disease caused by non-Hib in northwestern Ontario (38 cases between 2002 and 2008) with predominance of Hia was previously reported by the authors. At that time, no cases of pediatric meningitis caused byH influenzaewere recorded in the region. Continued surveillance identified 12 new cases of invasive non-Hib between January 2009 and July 2011. Among these cases, three young children developed meningitis with severe complications caused by Hia or Hif. The present article describes these cases along with the characteristics of recentH influenzaeisolates from the region, (ie, their genetic background and antibiotic sensitivity). The findings point to the clonal nature of circulating Hia strains as well as to an increase in frequency and severity of pediatric invasiveH influenzaeinfections in northwestern Ontario.

scholarly journals Subtyping of Haemophilus influenzaeStrains by Pulsed-Field Gel Electrophoresis

1999 ◽  
Vol 37 (7) ◽  
pp. 2142-2147 ◽  
Author(s):  
Mitsumasa Saito ◽  
Akiko Umeda ◽  
Shin-ichi Yoshida
Keyword(s):  
Cerebrospinal Fluid ◽  
Haemophilus Influenzae ◽  
Gel Electrophoresis ◽  
Invasive Disease ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Analysis ◽  
Pulsed Field ◽  
Pfge Typing ◽  
Household Contacts ◽  
Serotype B

A total of 200 isolates of Haemophilus influenzae were analyzed by serotyping, biotyping, and pulsed-field gel electrophoresis (PFGE). A total of 178 epidemiologically unrelated strains of H. influenzae demonstrated a variety of genome patterns by PFGE, and 165 genotypes were thus obtained in this study. PFGE typing proved to have a much stronger discriminatory power than either serotyping or biotyping. Six serotype b strains were all classified into discrete genotypes. A PFGE analysis of 18 strains obtained from the nasopharynx, blood, and cerebrospinal fluid of patients with meningitis also supported the hypothesis that invasive H. influenzaedisseminates from the nasopharynx to the bloodstream and then subsequently to other body sites. PFGE typing of 10 other strains isolated from household contacts of patients with H. influenzae infection revealed that the strain that caused theH. influenzae infection often colonized the nasopharynges of household contacts. Our findings suggest that PFGE analysis is useful for the epidemiological study of H. influenzaeinfection, even when the invasive disease is caused by serotype b strains.

scholarly journals Spontaneous capsule loss in Haemophilus influenzae serotype b associated with Hib conjugate vaccine failure and invasive disease

2019 ◽  
Vol 25 (3) ◽  
pp. 390-391 ◽  
Author(s):  
K. Meyler ◽  
M. Meehan ◽  
D. Bennett ◽  
R. Mulhall ◽  
O. Harrison ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Conjugate Vaccine ◽  
Invasive Disease ◽  
Vaccine Failure ◽  
Serotype B

Haemophilus influenzae serotype b and a capsule-deficient type mutant (b−) invasive disease in a partially vaccinated child in Brazil

2013 ◽  
Vol 62 (4) ◽  
pp. 655-657 ◽  
Author(s):  
Nathalia G. S. Caldeira ◽  
Ivano de Filippis ◽  
Tânia Catão Arruda ◽  
Maria Eulália Côrte Real ◽  
Alice Batalha de Jesus ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Invasive Disease ◽  
Type Mutant ◽  
Serotype B

scholarly journals Identification of Haemophilus influenzae Clones Associated with Invasive Disease a Decade after Introduction of H. influenzae Serotype b Vaccination in Italy

2013 ◽  
Vol 20 (8) ◽  
pp. 1223-1229 ◽  
Author(s):  
Maria Giufrè ◽  
Rita Cardines ◽  
Marisa Accogli ◽  
Manuela Pardini ◽  
Marina Cerquetti
Keyword(s):  
Haemophilus Influenzae ◽  
Promoter Region ◽  
Gene Promoter ◽  
Invasive Disease ◽  
Bacterial Disease ◽  
Related Sequence ◽  
Content Type ◽  
Iga1 Protease ◽  
Serotype B ◽  
Sequence Types

ABSTRACTThe introduction ofHaemophilus influenzaeserotype b (Hib) conjugate vaccines has changed the epidemiology of invasiveH. influenzaedisease, with a shift in the predominant serotype from Hib to nonencapsulatedH. influenzae(ncHi). The objective of this study was to identify the genotypes/clones associated with invasiveH. influenzaedisease in Italy. Eighty-sevenH. influenzaestrains isolated in the years 2009 to 2011 within the National Surveillance of Invasive Bacterial Disease program were analyzed. Strains were characterized by serotyping, antimicrobial susceptibility testing, and multilocus sequence typing (MLST). Genetic polymorphisms in theblaTEMgene promoter region as well as the occurrence of both adhesin genes (hmwAandhia) and the IgA1 protease-encoding gene (igaB) were also investigated. Of 87 strains, 67 were ncHi and 20 were encapsulated. Eleven strains were β-lactamase positive, harboring theblaTEMgene. MostblaTEMgenes (10/11) were associated with a Pdel promoter region exhibiting a 135-bp deletion; the remaining strain possessed the Pa/Pb overlapping promoter. MLST analysis showed that encapsulated isolates were clonal, with each serotype sharing a few related sequence types (STs). Forty-six different STs were identified among the 67 ncHi strains. Despite this heterogeneity, a group of closely related STs (ST103, ST139, and ST145) encompassed almost 25% of all ncHi strains and 45.5% of the β-lactamase producers carrying the Pdel promoter. These major ST clones were found to be associated with thehmwAgene but not with theigaBgene. To conclude, although the heterogeneity of the ncHi population was confirmed, diffusion of major successful ST clones was documented.

Clinical Characterization of Invasive Disease Caused by Haemophilus influenzae Serotype b in a High Vaccination Coverage Setting

2018 ◽  
Vol 8 (3) ◽  
pp. 261-264
Author(s):  
Susana Monge ◽  
Liesbeth Mollema ◽  
Hester de Melker ◽  
Elisabeth Sanders ◽  
Arie van der Ende ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Haemophilus Influenzae ◽  
Vaccination Coverage ◽  
Invasive Disease ◽  
National Study ◽  
Healthy Children ◽  
Disease Course ◽  
Serotype B

Abstract This national study characterized invasive Haemophilus influenzae serotype b infections. Vaccinated (n = 41) and nonvaccinated (n = 10) cases were similar regarding presentation as meningitis (68.8% vs 90.0%; P = .25), predisposing factors (29.3% vs 20.0%; P = .76), admission to intensive care unit or death (22.0% vs 10.0%; P = 1.00), or sequelae (21.6% vs 10.0%; P = .81). Haemophilus influenzae serotype b occurred in vaccinated, healthy children with comparable disease course.

scholarly journals Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Yesenia Osorio-Aguilar ◽  
Maria Cristina Gonzalez-Vazquez ◽  
Patricia Lozano-Zarain ◽  
Ygnacio Martinez-Laguna ◽  
Alejandro Carabarin-Lima ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Respiratory Infections ◽  
Polyclonal Antibodies ◽  
Wide Spectrum ◽  
Invasive Disease ◽  
Effective Vaccine ◽  
Binding Motif ◽  
Upper Respiratory Tract ◽  
Serotype B ◽  
Different Strains

Haemophilus influenzae is a common organism of the human upper respiratory tract; this bacterium is responsible of a wide spectrum for respiratory infections and can generate invasive diseases such as meningitis and septicemia. These infections are associated with H. influenzae encapsulated serotype b. However, the incidence of invasive disease caused by nontypeable H. influenzae (NTHi) has increased in the post-H. influenzae serotype b (Hib) vaccine era. Currently, an effective vaccine against NTHi is not available; due to this, it is important to find an antigen capable to confer protection against NTHi infection. In this study, 10 linear B cell epitopes and 13 CTL epitopes and a putative plasminogen-binding motif (252FYNKENGMY260) and the presence of enolase on the surface of different strains of H. influenzae were identified in the enolase sequence of H. influenzae. Both in silico and experimental results showed that recombinant enolase from H. influenzae is immunogenic that could induce a humoral immune response; this was observed mediating the generation of specific polyclonal antibodies anti-rNTHiENO that recognize typeable and nontypeable H. influenzae strains. The immunogenic properties and the superficial localization of enolase in H. influenzae, important characteristics to be considered as a new candidate for the development of a vaccine, were demonstrated.

Invasive disease due to Haemophilus influenzae serotype b ten years after routine vaccination, South Africa, 2003–2009

Vaccine ◽  
2012 ◽  
Vol 30 (3) ◽  
pp. 565-571 ◽  
Author(s):  
Anne von Gottberg ◽  
Cheryl Cohen ◽  
Andrew Whitelaw ◽  
Meera Chhagan ◽  
Brendan Flannery ◽  
...  
Keyword(s):  
South Africa ◽  
Haemophilus Influenzae ◽  
Invasive Disease ◽  
Routine Vaccination ◽  
Serotype B

Ultrastructural studies on the interaction of haemophilus influenzae with human endothelial cells in vitro

1990 ◽  
Vol 48 (3) ◽  
pp. 636-637
Author(s):  
D.J.P. Ferguson ◽  
M. Virji ◽  
H. Kayhty ◽  
E.R. Moxon
Keyword(s):  
Endothelial Cells ◽  
Haemophilus Influenzae ◽  
Intercellular Junctions ◽  
Blood Stream ◽  
Ultrastructural Studies ◽  
Endothelial Barriers ◽  
Serotype B ◽  
Meningitis In Children ◽  
Respiratory Epithelial

Haemophilus influenzae is a human pathogen which causes meningitis in children. Systemic H. influenzae infection is largely confined to encapsulated serotype b organisms and is a major cause of meningitis in the U.K. and elsewhere. However, the pathogenesis of the disease is still poorly understood. Studies in the infant rat model, in which intranasal challenge results in bacteraemia, have shown that H. influenzae enters submucosal tissues and disseminates to the blood stream within minutes. The rapidity of these events suggests that H. influenzae penetrates both respiratory epithelial and endothelial barriers with great efficiency. It is not known whether the bacteria penetrate via the intercellular junctions, are translocated within the cells or carried across the cellular barrier in 'trojan horse' fashion within phagocytes. In the present studies, we have challenged cultured human umbilical cord_vein endothelial cells (HUVECs) with both capsulated (b+) and capsule-deficient (b-) isogenic variants of one strain of H. influenzae in order to investigate the interaction between the bacteria and HUVEC and the effect of the capsule.

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