scholarly journals Reduction of Antibiotic Resistance Genes in Intestinal Microbiota of Patients With RecurrentClostridium difficileInfection After Fecal Microbiota Transplantation

2016 ◽  
Vol 63 (5) ◽  
pp. 710-711 ◽  
Author(s):  
Hanne Jouhten ◽  
Eero Mattila ◽  
Perttu Arkkila ◽  
Reetta Satokari
Keyword(s):  
Antibiotic Resistance ◽  
Intestinal Microbiota ◽  
Resistance Genes ◽  
Fecal Microbiota Transplantation ◽  
Antibiotic Resistance Genes ◽  
Fecal Microbiota

scholarly journals Fecal Microbiota Transplant in Cirrhosis Reduces Gut Microbial Antibiotic Resistance Genes: Analysis of Two Trials

2020 ◽  
Author(s):  
Jasmohan S. Bajaj ◽  
Amirhossein Shamsaddini ◽  
Andrew Fagan ◽  
Richard K. Sterling ◽  
Edith Gavis ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Fecal Microbiota ◽  
Fecal Microbiota Transplant

scholarly journals Integrons in the Intestinal Microbiota as Reservoirs for Transmission of Antibiotic Resistance Genes

Pathogens ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 238-248 ◽  
Author(s):  
Anuradha Ravi ◽  
Ekaterina Avershina ◽  
Jane Ludvigsen ◽  
Trine L'Abée-Lund ◽  
Knut Rudi
Keyword(s):  
Antibiotic Resistance ◽  
Intestinal Microbiota ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes

scholarly journals The shared resistome of human and pig microbiota is mobilized by distinct genetic elements

2020 ◽  
Author(s):  
Chao Wang ◽  
Yuqin Song ◽  
Na Tang ◽  
Gang Zhang ◽  
Sébastien Olivier Leclercq ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Intestinal Microbiota ◽  
Resistance Genes ◽  
Pathogenic Bacteria ◽  
Antibiotic Resistance Genes ◽  
Mobile Genetic Elements ◽  
Human Pathogens ◽  
Genetic Elements ◽  
Metagenomics Data ◽  
Genetic Context

The extensive use of antibiotics in hospitals and in the animal breeding industry has promoted antibiotic resistance in bacteria, which resulted in the emergence of a large number of antibiotic resistance genes in the intestinal tract of human and farmed animals. Genetic exchange of resistance genes between the two ecosystems is now well documented for pathogenic bacteria, but the repertoire of shared resistance genes in the commensal bacterial community and by which genetic modules they are disseminated are still unclear. By analyzing metagenomics data of human and pig intestinal samples both collected in Shenzhen, China, a set of 27 highly prevalent antibiotic resistance genes was found to be shared between human and pig intestinal microbiota. The mobile genetic context for 11 of these core antibiotic resistance genes could be identified by mining their carrying scaffolds constructed from the two datasets, leading to the detection of seven integrative and conjugative/mobilizable elements and two IS-related transposons. The comparison of the relative abundances between these detected mobile genetic elements and their associated antibiotic resistance genes revealed that for many genes, the estimated contribution of the mobile elements to the gene abundance differs strikingly depending on the host. These findings indicate that although some antibiotic resistance genes are ubiquitous across microbiota of human and pig populations, they probably relied on different genetic elements for their dissemination within each population. IMPORTANCE There is growing concern that antibiotic resistance genes could spread from the husbandry environment to human pathogens through dissemination mediated by mobile genetic elements. In this study, we investigated the contribution of mobile genetic elements to the abundance of highly prevalent antibiotic resistance genes found in commensal bacteria of both human and pig intestinal microbiota originating from the same region. Our results reveal that for most of these antibiotic resistance genes, the abundance is not explained by the same mobile genetic element in each host, suggesting that the human and pig microbial communities promoted a different set of mobile genetic carriers for the same antibiotic resistance genes. These results deepen our understanding of the dissemination of antibiotic resistance genes among and between human and pig gut microbiota.

Metagenome analysis of antibiotic resistance genes in fecal microbiota of chickens

Agri Gene ◽  
2017 ◽  
Vol 5 ◽  
pp. 1-6 ◽  
Author(s):  
Panpan Tong ◽  
Xue Ji ◽  
Lizhi Chen ◽  
Jun Liu ◽  
Lizhi Xu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Fecal Microbiota ◽  
Metagenome Analysis

scholarly journals An omics-based framework for assessing the health risk of antimicrobial resistance genes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
An-Ni Zhang ◽  
Jeffry M. Gaston ◽  
Chengzhen L. Dai ◽  
Shijie Zhao ◽  
Mathilde Poyet ◽  
...  
Keyword(s):  
High Risk ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Fecal Microbiota Transplantation ◽  
Antibiotic Resistance Genes ◽  
Fecal Microbiota ◽  
Clinical Applications ◽  
World Health ◽  
Antimicrobial Resistance Genes ◽  
Health Organization

AbstractAntibiotic resistance genes (ARGs) are widespread among bacteria. However, not all ARGs pose serious threats to public health, highlighting the importance of identifying those that are high-risk. Here, we developed an ‘omics-based’ framework to evaluate ARG risk considering human-associated-enrichment, gene mobility, and host pathogenicity. Our framework classifies human-associated, mobile ARGs (3.6% of all ARGs) as the highest risk, which we further differentiate as ‘current threats’ (Rank I; 3%) - already present among pathogens - and ‘future threats’ (Rank II; 0.6%) - novel resistance emerging from non-pathogens. Our framework identified 73 ‘current threat’ ARG families. Of these, 35 were among the 37 high-risk ARGs proposed by the World Health Organization and other literature; the remaining 38 were significantly enriched in hospital plasmids. By evaluating all pathogen genomes released since framework construction, we confirmed that ARGs that recently transferred into pathogens were significantly enriched in Rank II (‘future threats’). Lastly, we applied the framework to gut microbiome genomes from fecal microbiota transplantation donors. We found that although ARGs were widespread (73% of genomes), only 8.9% of genomes contained high-risk ARGs. Our framework provides an easy-to-implement approach to identify current and future antimicrobial resistance threats, with potential clinical applications including reducing risk of microbiome-based interventions.

Gut resistome presents a unique biome signature in chronic multi-symptom illness patients and links persistent pro-inflammatory phenotype in a mouse model reversible by fecal microbiota transfer

2021 ◽  
Author(s):  
Dipro Bose ◽  
Somdatta Chatterjee ◽  
Ethan Older ◽  
Ratanesh Seth ◽  
Patricia Janulewicz Lloyd ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Mouse Model ◽  
Resistance Genes ◽  
Intestinal Inflammation ◽  
Antibiotic Resistance Genes ◽  
Chronic Fatigue ◽  
Fecal Microbiota ◽  
Mouse Serum ◽  
War Veterans ◽  
Fecal Microbiota Transplant

Abstract Chronic multi-symptom illness (CMI) affects a subsection of elderly and war veterans and is associated with systemic inflammation, chronic fatigue, pain and neuroinflammation. We showed previously that an altered gut microbiome-inflammation axis aids to the symptom reporting and persistence. Here, a mouse model of CMI and a group of Gulf War veterans’ with CMI showed the presence of an altered host resistome, a signature of antibiotic resistance genes within the microbiome. Results showed that antibiotic resistance genes were significantly altered in the CMI group in both mice and GW veterans when compared to the control. Fecal samples from GW veterans with persistent CMI showed a significant increase of resistance to a wide class of antibiotics and exhibited an array of mobile genetic elements distinct than normal healthy controls. Strikingly, the altered resistome and gene signature were correlated with mouse serum IL6 levels. Altered resistome in mice also correlated strongly with intestinal inflammation, decreased synaptic plasticity that was reversible with fecal microbiota transplant (FMT), a tool to restore a healthy biome. The results indicate an emerging linkage of the gut resistome and CMI and might be significant in understanding the risks to treating hospital acquired infections in this population.

scholarly journals Infectious phage particles packaging antibiotic resistance genes found in meat products and chicken feces

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Clara Gómez-Gómez ◽  
Pedro Blanco-Picazo ◽  
Maryury Brown-Jaque ◽  
Pablo Quirós ◽  
Lorena Rodríguez-Rubio ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Genetic Material ◽  
Meat Products ◽  
Antibiotic Resistance Genes ◽  
Fecal Microbiota ◽  
Phage Dna ◽  
Before And After ◽  
Microbiological Parameters ◽  
Minced Meat

Abstract Bacteriophages can package part of their host’s genetic material, including antibiotic resistance genes (ARGs), contributing to a rapid dissemination of resistances among bacteria. Phage particles containing ARGs were evaluated in meat, pork, beef and chicken minced meat, and ham and mortadella, purchased in local retailer. Ten ARGs (blaTEM, blaCTX-M-1, blaCTX-M-9, blaOXA-48, blaVIM, qnrA, qnrS, mecA, armA and sul1) were analyzed by qPCR in the phage DNA fraction. The genes were quantified, before and after propagation experiments in Escherichia coli, to evaluate the ability of ARG-carrying phage particles to infect and propagate in a bacterial host. According to microbiological parameters, all samples were acceptable for consumption. ARGs were detected in most of the samples after particle propagation indicating that at least part of the isolated phage particles were infectious, being sul1the most abundant ARG in all the matrices followed by β-lactamase genes. ARGs were also found in the phage DNA fraction of thirty-seven archive chicken cecal samples, confirming chicken fecal microbiota as an important ARG reservoir and the plausible origin of the particles found in meat. Phages are vehicles for gene transmission in meat that should not be underestimated as a risk factor in the global crisis of antibiotic resistance.

scholarly journals Pediatric Fecal Microbiota Harbor Diverse and Novel Antibiotic Resistance Genes

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e78822 ◽  
Author(s):  
Aimée M. Moore ◽  
Sanket Patel ◽  
Kevin J. Forsberg ◽  
Bin Wang ◽  
Gayle Bentley ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Fecal Microbiota
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