scholarly journals Evaluation of Real-time Polymerase Chain Reaction for Detection of the 16S Ribosomal RNA Gene of Mycobacterium tuberculosis and the Diagnosis of Cervical Tuberculous Lymphadenitis in a Country With a High Tuberculosis Incidence

2012 ◽  
Vol 55 (3) ◽  
pp. 313-321 ◽  
Author(s):  
Patcharasarn Linasmita ◽  
Suthan Srisangkaew ◽  
Thanwa Wongsuk ◽  
Thongchai Bhongmakapat ◽  
Siriorn P. Watcharananan
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Tuberculous Lymphadenitis ◽  
Chain Reaction ◽  
Ribosomal Rna Gene ◽  
16S Ribosomal Rna Gene ◽  
Polymerase Chain

Cultural and molecular characterization of photobionts of Peltigera Membranacea

1997 ◽  
Vol 29 (6) ◽  
pp. 571-586 ◽  
Author(s):  
V.P.W. Mioa ◽  
A. Rabenau ◽  
A. Lee
Keyword(s):  
16S Rdna ◽  
Ribosomal Rna ◽  
Molecular Study ◽  
16S Ribosomal Rna ◽  
Chain Reaction ◽  
Ribosomal Rna Gene ◽  
Pcr Product ◽  
16S Ribosomal Rna Gene ◽  
Polymerase Chain

AbstractA molecular study was undertaken to clarify the identity of the photobiont in colourmorphs of the lichen, Peltigera membranacea. Two strains of cyanobacteria, identified as Nosroc sp. by morphology, were cultivated from each of two lichen specimens. Prokaryotic (16S) ribosomal RNA gene fragments were amplified by the polymerase chain reaction (PCR) from DNA extracted from the isolated strains and the lichens, and sequenced directly. Sequences were 98 1% identical between lichen specimens, TDI#AR94 and TDI#AR95, and highly similar to sequences published, or generated in this study from a type culture, for Nostoc. The 16S ribosomal RNA gene sequences (‘ 16S rDNA’) of all four lichen-derived cyanobacteria appeared the same, even though the lichen specimens from which they originated had different sequences. The 16S rDXA from strains 9A and 9B were different from that of specimen TDI#AR94, the thallus from which they were isolated, and instead were the same as that of strains 10A and 10B, and their source, specimen TDI#AR95. When primers selective for the strain 9A sequence were used, however, a small amount of PCR product corresponding to the 16S rDNA of strain 9A was obtained from lichen TDI#AR94. The results confirm that the photobionts of P. membranacea belong to Nostoc, and suggest that genetic differences in the photobiont may be a factor in the occurrence of colourmorphs among cyanolichens.

scholarly journals Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting

2016 ◽  
Vol 111 (9) ◽  
pp. 545-550 ◽  
Author(s):  
Juliana Failde Gallo ◽  
Juliana Maira Watanabe Pinhata ◽  
Erica Chimara ◽  
Maria Gisele Gonçalves ◽  
Lucila Okuyama Fukasawa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Chain Reaction ◽  
High Burden ◽  
Routine Diagnosis ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document