Cultural and molecular characterization of photobionts of Peltigera Membranacea

1997 ◽  
Vol 29 (6) ◽  
pp. 571-586 ◽  
Author(s):  
V.P.W. Mioa ◽  
A. Rabenau ◽  
A. Lee
Keyword(s):  
16S Rdna ◽  
Ribosomal Rna ◽  
Molecular Study ◽  
16S Ribosomal Rna ◽  
Chain Reaction ◽  
Ribosomal Rna Gene ◽  
Pcr Product ◽  
16S Ribosomal Rna Gene ◽  
Polymerase Chain

AbstractA molecular study was undertaken to clarify the identity of the photobiont in colourmorphs of the lichen, Peltigera membranacea. Two strains of cyanobacteria, identified as Nosroc sp. by morphology, were cultivated from each of two lichen specimens. Prokaryotic (16S) ribosomal RNA gene fragments were amplified by the polymerase chain reaction (PCR) from DNA extracted from the isolated strains and the lichens, and sequenced directly. Sequences were 98 1% identical between lichen specimens, TDI#AR94 and TDI#AR95, and highly similar to sequences published, or generated in this study from a type culture, for Nostoc. The 16S ribosomal RNA gene sequences (‘ 16S rDNA’) of all four lichen-derived cyanobacteria appeared the same, even though the lichen specimens from which they originated had different sequences. The 16S rDXA from strains 9A and 9B were different from that of specimen TDI#AR94, the thallus from which they were isolated, and instead were the same as that of strains 10A and 10B, and their source, specimen TDI#AR95. When primers selective for the strain 9A sequence were used, however, a small amount of PCR product corresponding to the 16S rDNA of strain 9A was obtained from lichen TDI#AR94. The results confirm that the photobionts of P. membranacea belong to Nostoc, and suggest that genetic differences in the photobiont may be a factor in the occurrence of colourmorphs among cyanolichens.

scholarly journals Comparison of 16S rDNA and 16S/23S Intergenic Region Sequences Among Citrus Greening Organisms in Asia

Plant Disease ◽  
2000 ◽  
Vol 84 (1) ◽  
pp. 15-18 ◽  
Author(s):  
Siti Subandiyah ◽  
Toru Iwanami ◽  
Shinji Tsuyumu ◽  
Hiroyuki Ieki
Keyword(s):  
16S Rdna ◽  
Ribosomal Rna ◽  
Intergenic Region ◽  
The Philippines ◽  
Citrus Greening ◽  
Chain Reaction ◽  
African Strain ◽  
16S Ribosomal Rna Gene ◽  
Polymerase Chain ◽  
India And China

Polymerase chain reaction was used to amplify and sequence the 16S ribosomal RNA gene (rDNA) and 16S/23S intergenic region of several isolates of citrus greening organism (GO) from Japan, the Philippines, Indonesia, and Thailand. The sequences of 16S rDNA were identical among all the isolates studied, very similar to the published sequences of Thai (99.4 to 100% identity), Nepalese (100% identity), and Indian (98.8% identity) strains, and less similar to an African strain (97.5% identity). The sequences of the intergenic region between 16S and 23S rDNA were also identical among the isolates examined as well as the reported Nepalese and Thai isolates. They were close to the sequences of reported strains of India and China (99.2%) and apart from those of the African strain (85.5%). These results suggested that some isolates of GO from Japan, the Philippines, Indonesia, Thailand, and Nepal constitute one strain, which is similar to Indian and Chinese strains and distinct from the African strain.

scholarly journals Influence of 16S Ribosomal RNA Gene Polymerase Chain Reaction and Sequencing on Antibiotic Management of Bone and Joint Infections

2013 ◽  
Vol 24 (2) ◽  
pp. 85-88 ◽  
Author(s):  
B Alraddadi ◽  
S Al-Azri ◽  
KR Forward
Keyword(s):  
16S Rrna ◽  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Pcr Assay ◽  
Ribosomal Rna Gene ◽  
Joint Infections ◽  
Bone And Joint Infections ◽  
Antibiotic Management ◽  
16S Ribosomal Rna Gene ◽  
Polymerase Chain

INTRODUCTION: Amplification of the 16S ribosomal RNA gene by polymerase chain reaction (PCR) followed by analysis of generated sequences can be an important adjunct to conventional cultures.OBJECTIVE: To determine how the results of this approach influence physicians’ decisions regarding the management of bone and joint infections.METHOD: Clinical and laboratory findings of patients seen at the Queen Elizabeth II Health Sciences Centre (Halifax, Nova Scotia) between December 2005 and September 2009 were reviewed. Patients who had negative cultures but likely or possible bone and joint infections were further evaluated using 16S rRNA PCR. The impact of the 16S rRNA PCR result on antibiotic management was evaluated and it was assessed whether untreated patients with negative 16S rRNA PCR subsequently presented with infections, suggesting a false-negative result.RESULT: A total of 36 patients (mean age 62 years) were reviewed. Thirty-two patients were evaluated by infectious disease consultants; of these, 20 were considered likely to have infections. Seventeen patients were admitted with suspected prosthetic joint infections. Twenty-nine patients received antimicrobial treatment before the sample for the 16S rRNA PCR assay was obtained. Of the 36 patients, 26 (72.2%) were treated appropriately with modifications to their antibiotic regimen in response to the 16S rRNA PCR assay results. Antimicrobials were discontinued for 19 patients based on negative PCR assay and, in seven patients, antibiotics were changed based on a positive result. There were no relapses among patients with negative PCR assay in whom antibiotics were discontinued.CONCLUSION: 16S ribosomal RNA gene PCR and sequencing is a valuable tool in the guidance of antimicrobial therapy for bone and joint infections.

scholarly journals Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing

2014 ◽  
Vol 45 (3) ◽  
pp. 985-993 ◽  
Author(s):  
Muhammad Naveed ◽  
Samavia Mubeen ◽  
SamiUllah khan ◽  
Iftikhar Ahmed ◽  
Nauman Khalid ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
Ribosomal Rna ◽  
Gene Sequencing ◽  
16S Ribosomal Rna ◽  
Ribosomal Rna Gene ◽  
16S Ribosomal Rna Gene ◽  
Identification And Characterization
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