scholarly journals Simultaneous Determination of Potassium Sorbate, Sodium Benzoate, Quinoline Yellow and Sunset Yellow in Lemonades and Lemon Sauces by HPLC Using Experimental Design

2016 ◽  
Vol 54 (6) ◽  
pp. 952-957 ◽  
Author(s):  
Şule Dinç Zor ◽  
Bürge Aşçı ◽  
Özlem Aksu Dönmez ◽  
Dilek Yıldırım Küçükkaraca
Keyword(s):  
Experimental Design ◽  
Simultaneous Determination ◽  
Sodium Benzoate ◽  
Potassium Sorbate ◽  
Sunset Yellow ◽  
Quinoline Yellow

Box-Behnken Design-Desirability Function Approach in Optimization of HPLC Method for Simultaneous Determination of Ibuprofen Along with Additives in Syrup Formulation

2020 ◽  
Author(s):  
Şule Dinç-Zor ◽  
Özlem Aksu Dönmez
Keyword(s):  
Simultaneous Determination ◽  
Flow Rate ◽  
Sodium Benzoate ◽  
Desirability Function ◽  
Hplc Method ◽  
Analysis Time ◽  
Statistical Experimental Design ◽  
Good Resolution ◽  
Sunset Yellow

Abstract Background The advantages of simultaneous analyses are a decrease in analysis time and a more economical use of solvents and reagents. It is desirable that HPLC analyses possess both short term and good resolution. Objective The aim of the current study is to develop an HPLC method for the simultaneous determination of ibuprofen, sodium benzoate, methyl paraben and propyl paraben as preservatives, and sunset yellow as a colorant, in syrup formulation. Method To optimize chromatographic separation conditions, multi-response optimization using the Derringer’s desirability function was employed for the development of a rapid and efficient HPLC method. The ranges of independent variables used for the optimization process were 50–60% (v/v) for acetonitrile, 5.0–7.0 for pH, and 1.0–2.0 mL/min for flow rate of the mobile phase. The effects of these variables on the output responses, such as critical resolution between sunset yellow and sodium benzoate and retention time of the last peak indicating analysis time of the method, were evaluated by statistical experimental design. Results Optimum conditions fixed for the simultaneous analyses were acetonitrile:phosphate buffer (60:40, v/v), pH 5.0, and a flow rate of 1.8 mL/min. The eluate was monitored using a photo diode detector set at 220 nm. Total chromatographic analysis time was approximately 3 min. Conclusions The developed method validated as per International Conference on Harmonization guidelines was successfully applied for the determination of five compounds in their pharmaceutical formulation. Highlights This efficient method has isocratic elution system and can be used for routine analyses of these compounds in similar pharmaceutical products.

scholarly journals Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLC

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Hakan Alp ◽  
Davut Başkan ◽  
Ahmet Yaşar ◽  
Nurettin Yaylı ◽  
Ümmühan Ocak ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Food Samples ◽  
Sunset Yellow ◽  
Allura Red ◽  
Rp Hplc ◽  
Relative Standard ◽  
Quinoline Yellow

An efficient method was developed for the simultaneous determination of Sunset Yellow FCF (E110), Allura Red AC (E129), Quinoline Yellow WS (E104), and Tartrazine (E102) in food samples by RP-HPLC. The mentioned food dyes were analyzed at room temperature for 23 min with gradient elution. Three mobile phases were used for the elution, and mobile phase A was an acetate buffer (pH = 7.5, 1%), mobile phase B was acetonitrile, and mobile phase C was methanol. The flow rate was 1.0 mL min−1, and the injection volume was 20 µL. The linear ranges were 0.72–50 mg L−1, 0.24–50 mg L−1, 0.75–10 mg L−1, and 0.69–50 mg L−1for Tartrazine, Quinoline Yellow WS, Sunset Yellow FCF, and Allura Red AC, respectively.R2values were 0.999 for all dyes. Limits of detection were 0.24 mg L−1, 0.08 mg L−1, 0.25 mg L−1, and 0.23 mg L−1for Tartrazine, Quinoline Yellow WS, Sunset Yellow FCF, and Allura Red AC, respectively. The relative standard deviation (RSD) of the measurements for all of the four dyes was between 0.56 and 1.65% intraday measurements. This method was successfully applied in the determination of the mentioned dyes in ice pops, gummy bears, chewing gum, and sweets candy samples.

Simultaneous determination of streptomycin and oxytetracycline in agricultural antimicrobials by CZE after an experimental design

2007 ◽  
Vol 43 (2) ◽  
pp. 450-456 ◽  
Author(s):  
Patrícia Penido Maia ◽  
Jaime Amaya-Farfán ◽  
Susanne Rath ◽  
Felix Guillermo Reyes Reyes
Keyword(s):  
Experimental Design ◽  
Simultaneous Determination
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