scholarly journals Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLC

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Hakan Alp ◽  
Davut Başkan ◽  
Ahmet Yaşar ◽  
Nurettin Yaylı ◽  
Ümmühan Ocak ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Food Samples ◽  
Sunset Yellow ◽  
Allura Red ◽  
Rp Hplc ◽  
Relative Standard ◽  
Quinoline Yellow

An efficient method was developed for the simultaneous determination of Sunset Yellow FCF (E110), Allura Red AC (E129), Quinoline Yellow WS (E104), and Tartrazine (E102) in food samples by RP-HPLC. The mentioned food dyes were analyzed at room temperature for 23 min with gradient elution. Three mobile phases were used for the elution, and mobile phase A was an acetate buffer (pH = 7.5, 1%), mobile phase B was acetonitrile, and mobile phase C was methanol. The flow rate was 1.0 mL min−1, and the injection volume was 20 µL. The linear ranges were 0.72–50 mg L−1, 0.24–50 mg L−1, 0.75–10 mg L−1, and 0.69–50 mg L−1for Tartrazine, Quinoline Yellow WS, Sunset Yellow FCF, and Allura Red AC, respectively.R2values were 0.999 for all dyes. Limits of detection were 0.24 mg L−1, 0.08 mg L−1, 0.25 mg L−1, and 0.23 mg L−1for Tartrazine, Quinoline Yellow WS, Sunset Yellow FCF, and Allura Red AC, respectively. The relative standard deviation (RSD) of the measurements for all of the four dyes was between 0.56 and 1.65% intraday measurements. This method was successfully applied in the determination of the mentioned dyes in ice pops, gummy bears, chewing gum, and sweets candy samples.

scholarly journals Development and Validation of RP-HPLC and an Ecofriendly HPTLC Method for Simultaneous Determination of Felodipine and Metoprolol Succinate, and their Major Metabolites in Human Spiked Plasma

2020 ◽  
Vol 103 (4) ◽  
pp. 966-971
Author(s):  
Aml A Emam ◽  
Ibrahim A Naguib ◽  
Eman S Hassan ◽  
Eglal A Abdelaleem
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Metoprolol Succinate ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Spiked Plasma ◽  
Development And Validation ◽  
Hptlc Method

Abstract Background Felodipine is a calcium channel blocker used together with metoprolol succinate for treatment of hypertension. Objective Two chromatographic methods were developed for simultaneous determination of felodipine (FEL) and metoprolol succinate (MET), and their major metabolites, dehydrofelodipine and metoprolol acid, respectively. Methods The first method was RP-HPLC which comprised separation of the studied components by gradient elution using a Phenomenex C8 column and a mobile phase composed of water (adjusted to pH 3.5 with o-phosphoric acid)–acetonitrile – methanol (45:40:15, by volume) for the first 6 min and (30:60:10, by volume) for the next 4 min at a flow rate of 1 mL/min followed by UV detection of the eluted peaks at 225 nm. The second method was an HPTLC method where separation was achieved using a mobile phase consisting of toluene–ethyl acetate–methanol–ammonia–formic acid (10:5:2.5:0.3:0.1, by volume) and scanning of the separated bands at 225 nm. Results Validation of the developed methods was done according to ICH guidelines. Successful application of the developed methods was carried out for determination of the studied drugs in human spiked plasma and in Logimax® tablets. Conclusions The developed RP-HPLC and HPTLC methods can be further applied for quality control testing of the studied drugs. Highlights RP-HPLC and HPTLC methods for determination of FEL, MET and their major metabolites. The developed methods were successfully applied for determination of FEL and MET in Logimax® tablets.

scholarly journals Simultaneous determination of chlorpheniramine maleate, phenylephrine hydrochloride, paracetamol and caffeine in pharmaceutical preparation by RP-HPLC

2013 ◽  
Vol 19 (1) ◽  
pp. 57-65 ◽  
Author(s):  
V.K. Redasani ◽  
A.P. Gorle ◽  
R.A. Badhan ◽  
P.S. Jain ◽  
S.J. Surana
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Pharmaceutical Preparation ◽  
Reversed Phase ◽  
Chlorpheniramine Maleate ◽  
Phenylephrine Hydrochloride ◽  
Rp Hplc ◽  
Relative Standard

Reversed-phase High-Performance Liquid-Chromatography (RP-HPLC) method was successfully developed for the simultaneous determination of quaternary mixture consisting of chlorpheniramine maleate (CPM), phenylephrine hydrochloride (PE), paracetamol (PCM) and caffeine in pharmaceutical preparation. The method was found to be simple, sensitive and rapid. The separation of the drugs was carried out using Inertsil ODS C18 column using 0.05M dibasic phosphate buffer: acetonitrile (93: 07; v/v) as mobile phase. The flow rate of mobile phase was adjusted to 1.5 ml/min and column oven temperature was kept at 30?C. All these drugs were resolved successfully with retention times 2.74 (CPM), 3.48(PE), 9.5(PCM) and 26.32(Caffeine) minutes when detection was carried out at 215 nm. Correlation coefficient was found 0.999, 0.998, 0.999 and 0.999 respectively for CPM, PE, PCM and Caffeine. The relative standard deviation in the tablets was found less than 2% for six replicates. The method was validated for precision and accuracy. Thus, proposed method can be successfully applicable to the pharmaceutical preparation containing the above mentioned drugs without any interference of excipients.

scholarly journals Development and validation of reversed phase high performance liquid chromatography (RP-HPLC) for quantification of captopril in rabbit plasma

2020 ◽  
Author(s):  
Muhammad Fawad Rasool ◽  
Umbreen Fatima Qureshi ◽  
Nazar Muhammad Ranjha ◽  
Imran Imran ◽  
Mouqadus Un Nisa ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Limit Of Detection ◽  
Reversed Phase ◽  
Rabbit Plasma ◽  
Rp Hplc ◽  
Relative Standard

AbstractTh accurate rapid, simple and selective reversed phase high performance liquid chromatography (RP-HPLC) has been established and validated for the determination of captopril (CAP). Chromatographic separation was accomplished using prepacked ODSI C18 column (250 mm × 4.6 mm with 5 μm particle size) in isocratic mode, with mobile phase consisting of water: acetonitrile (60:40 v/v), pH adjusted to 2.5 by using 85% orthophosphoric acid at a flow rate of 1 mL/min and UV detection was performed at 203 nm. RP-HPLC method used for the analysis of CAP in mobile phase and rabbit plasma was established and validated as per ICH-guidelines. It was carried out on a well-defined chromatographic peak of CAP was established with a retention time of 4.9 min and tailing factor of 1.871. The liquid–liquid extraction method was used for extraction of CAP from the plasma. Excellent linearity (R2 = 0.999) was shown over range 3.125–100 µg/mL with mean percentage recoveries ranges from 97 to 100.6%. Parameters of precision and accuracy of the developed method meet the established criteria. Intra and inter-day precision (% relative standard deviation) study was also performed which was less than 2% which indicate good reproducibility of the method. The limit of detection (LOD) and quantification for the CAP in plasma were 3.10 and 9.13 ng/mL respectively. The method was suitably validated and successfully applied to the determination of CAP in rabbit plasma samples.

Simultaneous determination of five pesticides residues in Anoectochilus roxburghii by HPLC-MS/MS method

2020 ◽  
Vol 20 (5) ◽  
pp. 345-349
Author(s):  
Liqun WU ◽  
◽  
Mingke LUO ◽  
Wensheng HE ◽  
Wenting CHEN
Keyword(s):  
Simultaneous Determination ◽  
Multiple Reaction Monitoring ◽  
Gradient Elution ◽  
Acetonitrile Solution ◽  
Ionization Source ◽  
Thiophanate Methyl ◽  
Pesticides Residues ◽  
Relative Standard ◽  
Anoectochilus Roxburghii

Objective： To establish a HPLC-MS/MS method for simultaneous determination of five pesticides residues including tebuconazole，metalaxyl-M，thiophanate-methyl，oxytetracycline and abamectin in Anoectochilus roxburghii.Methods： The samples were extracted by acetonitrile solution and purified by QuEChERS method.Separation was performed on Agilent Eclipse Plus C18 column（50 mm×2.1 mm，1.8 μm） with the mobile phase consisting of 0.1% formic acid（A） and acetonitrile（B） by gradient elution（0-1.0 min，90% A；1.0-3.0 min，90%-70% A;3.0-5.0 min，70%-30% A;5.0-8.0 min，30%-10% A;8.0-14.0 min，10% A;14.0-15.0 min，10%-90% A;15.0-17.0 min，90% A）.Detection was performed on a triple quadrupole tandem mass spectrometry with electrospray ionization source operating in positive ion mode under multiple reaction monitoring（MRM） mode.Results： The calibration curves of five pesticides showed good linearity coefficients（r>0.99）.The average recoveries ranged from 72.7% to 94.9%（n=6），and the relative standard derivations（RSDs） were within 1.8%-10.3%（n=6）.The detection limits of tebuconazole，metalaxyl-M，thiophanate-methyl，oxytetracycline and abamectin were 0.6，0.2，0.3，0.2，5.0 μg/kg，and the quantitation limits of 5 pesticides were 2.0，0.5，1.0，0.6 and 10.0 μg/kg respectively.Conclusion： The established method was proved to be sensitive，simple and reliable，and could be applied for the determination of these five pesticides in Anoectochilus roxburghii and quality control in the planting process.

Development of a Method for Simultaneous Determination of Two Stilbenes and Four Anthraquinones from Polygonum Cuspidatum by RP-HPLC

2019 ◽  
Vol 102 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Jianye Yan ◽  
Yuanqing Wang ◽  
Hongnian Wu ◽  
Zhicheng Sun ◽  
Shihan Tan ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Polygonum Cuspidatum ◽  
Chinese Medicinal Herb ◽  
Simple Method ◽  
Rp Hplc ◽  
First Time

Abstract Background: Polygonum Cuspidatum Sieb. et Zucc. (named Huzhang in China) is a traditional and popular Chinese medicinal herb used in removing jaundice, clearing heat-toxin, improving blood circulation, expelling stasis, dispelling wind and dampness, repelling phlegm, and suppressing cough. It is widely used in drug and functional food fields and distributed throughout the world, including in China, Japan, and North America. Objective: To control the quality of Polygonum Cuspidatum, an effective, reliable, and simple method for simultaneous determination of two stilbenes (polydatin, resveratrol) and four anthraquinones (emodin, physcion, rhein, and anthraglycoside B) was developed and validated for the first time in this study by reversed-phase HPLC (RP-HPLC). Methods: Separation was carried out on Agilent C18 column (250 × 4.6 mm I.D., 5 μm) with acetonitrile and 0.10% aqueous phosphoric acid as mobile phase and gradient elution at a flow rate of 0.8 mL/min. Detection was conducted with mobile wavelength at 30°C. Results: Good validation of the method including linearity, precision, repeatability, and recovery was performed. The contents of the studied analytes are significantly different, and resveratrol and rhein in particular existed in greater fluctuation among the samples. Conclusion: A simple, reliable, and sensitive method has been successfully established and applied to the analysis for simultaneous determination of the target compounds in 11 batches of samples. Highlights: Separation and quantitative analysis of two stilbenes and four anthraquinones from P. cuspidatum were developed by RP-HPLC. This method is convenient, sensitive, and accurate and can provide a reliable basis for further applications of P. cuspidatum in drug or food fields.

STANDARDIZATION OF HYGROPHILA SPINOSA AND ITS FORMULATION WITH REFERENCE TO LUPEOL, BY DEVELOPED AND VALIDATED HPTLC AND RP-HPLC METHODS

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (01) ◽  
pp. 13-19
Author(s):  
R Jayaprakasam ◽  
◽  
M. F. Saleshier ◽  
T. K. Ravi
Keyword(s):  
Standard Deviation ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Solvent System ◽  
Good Recovery ◽  
Rp Hplc ◽  
Relative Standard ◽  
Optimum Wavelength ◽  
Hptlc Method

The separation and determination of lupeol from Hygrophila spinosa were carried out by two simple, precise and accurate HPTLC and RP-HPLC methods. HPTLC method for the determination of lupeol from plant extract and its formulation was developed using a solvent system consisting of toluene : ethyl acetate : methanol (15: 3: 1.5%v/v/v). For detection, lupeol had to be derivatized with Liebermann Burchard reagent at 1050C. The optimum wavelength was fixed as 366nm. In RP-HPLC, the separation was carried out on a C18 column and the mobile phase selected was methanol: acetonitrile (30:70%v/v). The maximum wavelength was found to be 210nm.The method was validated in terms of various parameters. Low relative standard deviation and good % recovery values of both the methods showed that the developed methods were highly precise and accurate and therefore can be used for the standardization and quantification of lupeol in Hygrophila spinosa and its formulation.

Sign in / Sign up

Export Citation Format

Share Document