scholarly journals Nitric oxide is the shared signalling molecule in phosphorus- and iron-deficiency-induced formation of cluster roots in white lupin (Lupinus albus)

2012 ◽  
Vol 109 (6) ◽  
pp. 1055-1064 ◽  
Author(s):  
Zhi Bin Meng ◽  
Li Qian Chen ◽  
Dong Suo ◽  
Gui Xin Li ◽  
Cai Xian Tang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Iron Deficiency ◽  
Lupinus Albus ◽  
White Lupin ◽  
Cluster Roots ◽  
Signalling Molecule

scholarly journals Nodulated White Lupin Plants Growing in Contaminated Soils Accumulate Unusually High Mercury Concentrations in Their Nodules, Roots and Especially Cluster Roots

Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 302
Author(s):  
Miguel A. Quiñones ◽  
Susana Fajardo ◽  
Mercedes Fernández-Pascual ◽  
M. Mercedes Lucas ◽  
José J. Pueyo
Keyword(s):  
Contaminated Soils ◽  
Lupinus Albus ◽  
White Lupin ◽  
Cluster Roots ◽  
Cluster Root ◽  
Bioaccumulation Factors ◽  
Aerial Parts ◽  
High Mercury ◽  
Lupinus Albus L ◽  
Hg Accumulation

Two white lupin (Lupinus albus L.) cultivars were tested for their capacity to accumulate mercury when grown in Hg-contaminated soils. Plants inoculated with a Bradyrhizobium canariense Hg-tolerant strain or non-inoculated were grown in two highly Hg-contaminated soils. All plants were nodulated and presented a large number of cluster roots. They accumulated up to 600 μg Hg g−1 DW in nodules, 1400 μg Hg g−1 DW in roots and 2550 μg Hg g−1 DW in cluster roots. Soil, and not cultivar or inoculation, was accountable for statistically significant differences. No Hg translocation to leaves or seeds took place. Inoculated L. albus cv. G1 plants were grown hydroponically under cluster root-promoting conditions in the presence of Hg. They accumulated about 500 μg Hg g−1 DW in nodules and roots and up to 1300 μg Hg g−1 DW in cluster roots. No translocation to the aerial parts occurred. Bioaccumulation factors were also extremely high, especially in soils and particularly in cluster roots. To our knowledge, Hg accumulation in cluster roots has not been reported to date. Our results suggest that inoculated white lupin might represent a powerful phytoremediation tool through rhizosequestration of Hg in contaminated soils. Potential uptake and immobilization mechanisms are discussed.

Is there a critical level of shoot phosphorus concentration for cluster-root formation in Lupinus albus?

2008 ◽  
Vol 35 (4) ◽  
pp. 328 ◽  
Author(s):  
Haigang Li ◽  
Jianbo Shen ◽  
Fusuo Zhang ◽  
Caixian Tang ◽  
Hans Lambers
Keyword(s):  
Organic Matter ◽  
Root Formation ◽  
Critical Level ◽  
Lupinus Albus ◽  
White Lupin ◽  
Cluster Roots ◽  
Proton Release ◽  
P Concentration ◽  
Citrate Exudation ◽  
P Supply

This study examined the effects of localised phosphorus (P) supply on cluster-root formation and citrate exudation in white lupin (Lupinus albus L. cv. Kiev Mutant). White lupin plants were grown in nutrient solutions with a range of P supplies in a split-root system with one root half deprived of P and the other root supplied with 0, 2, 5, 8, 10 or 75 μm P. Plants were also grown in soil with or without organic matter added to the top layer. The proportion of cluster roots as a percentage of the total root biomass decreased similarly on both root halves with increasing P supply in the hydroponic experiments. More than 18% of the P taken up by the P-supplied root halves was incorporated into the P-deprived halves. Irrespective of the P supply or organic matter addition in the experiments, the proportion of cluster roots and the rate of citrate exudation decreased sharply with increasing P concentration in the shoots up to a critical level of 2–3 mg P g–1 dry weight. In contrast, the rate of proton release was higher in P-deprived root halves than in P-supplied ones. The formation of cluster roots is regulated by shoot P concentration with a critical level of 2–3 mg g–1. Citrate exudation is predominantly governed by shoot P status, whereas proton release strongly responds to local P supply.

scholarly journals Genome sequence of the cluster root forming white lupin

2019 ◽  
Author(s):  
Bárbara Hufnagel ◽  
André Marques ◽  
Alexandre Soriano ◽  
Laurence Marquès ◽  
Fanchon Divol ◽  
...  
Keyword(s):  
Genome Sequence ◽  
De Novo ◽  
Lateral Roots ◽  
Lupinus Albus ◽  
White Lupin ◽  
Cluster Roots ◽  
Mycorrhizal Symbiosis ◽  
High Quality ◽  
Soil Exploration ◽  
High Quality Genome

White lupin (Lupinus albus L.) is a legume that produces seeds recognized for their high protein content and good nutritional value (lowest glycemic index of all grains, high dietary fiber content, and zero gluten or starch)1–5. White lupin can form nitrogen-fixing nodules but has lost the ability to form mycorrhizal symbiosis with fungi6. Nevertheless, its root system is well adapted to poor soils: it produces cluster roots, constituted of dozens of determinate lateral roots that improve soil exploration and phosphate remobilization7. As phosphate is a limited resource that comes from rock reserves8, the production of cluster roots is a trait of interest to improve fertilizers efficiency. Using long reads sequencing technologies, we provide a high-quality genome sequence of a modern variety of white lupin (2n=50, 451 Mb), as well as de novo assemblies of a landrace and a wild relative. We describe how domestication impacted soil exploration capacity through the early establishment of lateral and cluster roots. We identify the APETALA2 transcription factor LaPUCHI-1, homolog of the Arabidopsis morphogenesis coordinator9, as a potential regulator of this trait. Our high-quality genome and companion genomic and transcriptomic resources enable the development of modern breeding strategies to increase and stabilize yield and to develop new varieties with reduced allergenic properties (caused by conglutins10), which would favor the deployment of this promising culture.

Iron deficiency induces changes in metabolism of citrate in lateral roots and cluster roots of Lupinus albus

2004 ◽  
Vol 121 (4) ◽  
pp. 586-594 ◽  
Author(s):  
Joyce McCluskey ◽  
Lindsey Herdman ◽  
Keith Ronald Skene
Keyword(s):  
Iron Deficiency ◽  
Lateral Roots ◽  
Lupinus Albus ◽  
Cluster Roots

scholarly journals Nitrogen and Phosphorus Interplay in Lupin Root Nodules and Cluster Roots

2021 ◽  
Vol 12 ◽  
Author(s):  
José J. Pueyo ◽  
Miguel A. Quiñones ◽  
Teodoro Coba de la Peña ◽  
Elena E. Fedorova ◽  
M. Mercedes Lucas
Keyword(s):  
Root Nodules ◽  
Lupinus Albus ◽  
Nutrient Deficiency ◽  
White Lupin ◽  
Cluster Roots ◽  
Nitrogen And Phosphorus ◽  
Signaling Mechanisms ◽  
Plant Acclimation ◽  
Interactive Regulation ◽  
The Individual

Nitrogen (N) and phosphorus (P) are two major plant nutrients, and their deficiencies often limit plant growth and crop yield. The uptakes of N or P affect each other, and consequently, understanding N–P interactions is fundamental. Their signaling mechanisms have been studied mostly separately, and integrating N–P interactive regulation is becoming the aim of some recent works. Lupins are singular plants, as, under N and P deficiencies, they are capable to develop new organs, the N2-fixing symbiotic nodules, and some species can also transform their root architecture to form cluster roots, hundreds of short rootlets that alter their metabolism to induce a high-affinity P transport system and enhance synthesis and secretion of organic acids, flavonoids, proteases, acid phosphatases, and proton efflux. These modifications lead to mobilization in the soil of, otherwise unavailable, P. White lupin (Lupinus albus) represents a model plant to study cluster roots and for understanding plant acclimation to nutrient deficiency. It tolerates simultaneous P and N deficiencies and also enhances uptake of additional nutrients. Here, we present the structural and functional modifications that occur in conditions of P and N deficiencies and lead to the organogenesis and altered metabolism of nodules and cluster roots. Some known N and P signaling mechanisms include different factors, including phytohormones and miRNAs. The combination of the individual N and P mechanisms uncovers interactive regulation pathways that concur in nodules and cluster roots. L. albus interlinks N and P recycling processes both in the plant itself and in nature.

Nitric Oxide in Life and Death of Neutrophils

2019 ◽  
Vol 26 (31) ◽  
pp. 5764-5780 ◽  
Author(s):  
Svetlana I. Galkina ◽  
Ekaterina A. Golenkina ◽  
Galina M. Viryasova ◽  
Yulia M. Romanova ◽  
Galina F. Sud’ina
Keyword(s):  
Nitric Oxide ◽  
Cell Damage ◽  
Protective Role ◽  
Neutrophil Apoptosis ◽  
High Biological Activity ◽  
Life And Death ◽  
Signalling Molecule ◽  
Activated Neutrophils ◽  
Cell Death Mechanisms ◽  
Prostacyclin Synthase

Background: Nitric Oxide (NO) is a key signalling molecule that has an important role in inflammation. It can be secreted by endothelial cells, neutrophils, and other cells, and once in circulation, NO plays important roles in regulating various neutrophil cellular activities and fate. Objective: To describe neutrophil cellular responses influenced by NO and its concomitant compound peroxynitrite and signalling mechanisms for neutrophil apoptosis. Methods: Literature was reviewed to assess the effects of NO on neutrophils. Results: NO plays an important role in various neutrophil cellular activities and interaction with other cells. The characteristic cellular activities of neutrophils are adhesion and phagocytosis. NO plays a protective role in neutrophil-endothelial interaction by preventing neutrophil adhesion and endothelial cell damage by activated neutrophils. NO suppresses neutrophil phagocytic activity but stimulates longdistance contact interactions through tubulovesicular extensions or cytonemes. Neutrophils are the main source of superoxide, but NO flow results in the formation of peroxynitrite, a compound with high biological activity. Peroxynitrite is involved in the regulation of eicosanoid biosynthesis and inhibits endothelial prostacyclin synthase. NO and peroxynitrite modulate cellular 5-lipoxygenase activity and leukotriene synthesis. Long-term exposure of neutrophils to NO results in the activation of cell death mechanisms and neutrophil apoptosis. Conclusion: Nitric oxide and the NO/superoxide interplay fine-tune mechanisms regulating life and death in neutrophils.

scholarly journals Quantitative Control of Early Flowering in White Lupin (Lupinus albus L.)

2021 ◽  
Vol 22 (8) ◽  
pp. 3856
Author(s):  
Sandra Rychel-Bielska ◽  
Anna Surma ◽  
Wojciech Bielski ◽  
Bartosz Kozak ◽  
Renata Galek ◽  
...  
Keyword(s):  
Flowering Time ◽  
Association Studies ◽  
Lupinus Albus ◽  
Genotypic Diversity ◽  
Early Flowering ◽  
White Lupin ◽  
Genome Wide Association Studies ◽  
Interacting Protein ◽  
Major Qtls ◽  
Lupinus Albus L

White lupin (Lupinus albus L.) is a pulse annual plant cultivated from the tropics to temperate regions for its high-protein grain as well as a cover crop or green manure. Wild populations are typically late flowering and have high vernalization requirements. Nevertheless, some early flowering and thermoneutral accessions were found in the Mediterranean basin. Recently, quantitative trait loci (QTLs) explaining flowering time variance were identified in bi-parental population mapping, however, phenotypic and genotypic diversity in the world collection has not been addressed yet. In this study, a diverse set of white lupin accessions (n = 160) was phenotyped for time to flowering in a controlled environment and genotyped with PCR-based markers (n = 50) tagging major QTLs and selected homologs of photoperiod and vernalization pathway genes. This survey highlighted quantitative control of flowering time in white lupin, providing statistically significant associations for all major QTLs and numerous regulatory genes, including white lupin homologs of CONSTANS, FLOWERING LOCUS T, FY, MOTHER OF FT AND TFL1, PHYTOCHROME INTERACTING FACTOR 4, SKI-INTERACTING PROTEIN 1, and VERNALIZATION INDEPENDENCE 3. This revealed the complexity of flowering control in white lupin, dispersed among numerous loci localized on several chromosomes, provided economic justification for future genome-wide association studies or genomic selection rather than relying on simple marker-assisted selection.

Nitric oxide ameliorates the damaging effects of oxidative stress induced by iron deficiency in cyanobacterium Anabaena 7120

2016 ◽  
Vol 23 (21) ◽  
pp. 21805-21821 ◽  
Author(s):  
Manish Singh Kaushik ◽  
Meenakshi Srivastava ◽  
Alka Srivastava ◽  
Anumeha Singh ◽  
Arun Kumar Mishra
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Iron Deficiency ◽  
Anabaena 7120 ◽  
Cyanobacterium Anabaena ◽  
Damaging Effects
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