An Improved DNA Vaccine Against Bovine Herpesvirus-1 Using CD40L and a Chemical Adjuvant Induces Specific Cytotoxicity in Mice

2020 ◽  
Author(s):  
Cecilia A. Langellotti ◽  
Mariela Gammella ◽  
Ivana Soria ◽  
Carolina Bellusci ◽  
Valeria Quattrocchi ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Specific Cytotoxicity ◽  
Herpesvirus 1

Induction of immune responses in cattle with a DNA vaccine encoding glycoprotein C of bovine herpesvirus-1

2001 ◽  
Vol 78 (4) ◽  
pp. 293-305 ◽  
Author(s):  
Praveen K. Gupta ◽  
Mohini Saini ◽  
L.K. Gupta ◽  
V.D.P. Rao ◽  
S.K. Bandyopadhyay ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1

Intercellular trafficking of the major tegument protein VP22 of bovine herpesvirus-1 and its application to improve a DNA vaccine

2005 ◽  
Vol 151 (5) ◽  
pp. 985-993 ◽  
Author(s):  
C. F. Zheng ◽  
R. Brownlie ◽  
D. Y. Huang ◽  
L. A. Babiuk ◽  
S. van Drunen Littel-van den Hurk
Keyword(s):  
Dna Vaccine ◽  
Bovine Herpesvirus ◽  
Tegument Protein ◽  
Bovine Herpesvirus 1 ◽  
Intercellular Trafficking ◽  
Herpesvirus 1 ◽  
Protein Vp22

scholarly journals Immunization with a bovine herpesvirus 1 glycoprotein B DNA vaccine induces cytotoxic T-lymphocyte responses in mice and cattle

2005 ◽  
Vol 86 (4) ◽  
pp. 887-898 ◽  
Author(s):  
Y. Huang ◽  
L. A. Babiuk ◽  
S. van Drunen Littel-van den Hurk
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Cytotoxic T Lymphocyte ◽  
Bovine Herpesvirus ◽  
Plasmid Vector ◽  
Injection System ◽  
Glycoprotein B ◽  
Bovine Herpesvirus 1 ◽  
Ctl Response ◽  
Herpesvirus 1

Virus-specific cytotoxic T lymphocytes (CTLs) are considered to be important in protection against and recovery from viral infections. In this study, several approaches to induce cytotoxicity against bovine herpesvirus 1 (BHV-1) were evaluated. Vaccination of C57BL/6 mice with BHV-1 induced a strong humoral, but no CTL, response, which may be due to downregulation of major histocompatibility complex class I molecules. In contrast, vaccinia virus expressing glycoprotein B (gB) elicited a weaker antibody response, but strong cytotoxicity, in mice. As an approach to inducing both strong humoral and cellular immune responses, a plasmid vector was then used to express gB. Both antibody and CTL responses were induced by the plasmid encoding gB in C57BL/6 and C3H mice, regardless of the type of vector backbone. This demonstrated that DNA immunization induces a broad-based immune response to BHV-1 gB. Interestingly, removal of the membrane anchor, which resulted in secretion of gB from transfected cells, did not result in reduced cytotoxicity. Here, it is shown that, compared with the cell-associated counterpart, plasmid-encoded secreted protein may induce enhanced immune responses in cattle. Therefore, calves were immunized intradermally with pMASIAtgB, a plasmid encoding the secreted form of gB (tgB), using a needle-free injection system. This demonstrated that pMASIAtgB elicited both humoral responses and activated gamma interferon-secreting CD8+ CTLs, suggesting that a DNA vaccine expressing tgB induces a CTL response in the natural host of BHV-1.

A DNA vaccine encoding the FMDV capsid precursor polypeptide P1 and the enhancing effect of bovine herpesvirus 1 VP22 protein as molecular adjuvant

2012 ◽  
Vol 56 (02) ◽  
pp. 111-117 ◽  
Author(s):  
XIAOLAN YU ◽  
SHAOBO XIAO ◽  
YUNBO JIANG ◽  
LIURONG FANG ◽  
HUANCHUN CHEN ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Enhancing Effect ◽  
Capsid Precursor ◽  
Molecular Adjuvant ◽  
Herpesvirus 1

scholarly journals Intranasal delivery of plasmids expressing bovine herpesvirus 1 gB/gC/gD proteins by polyethyleneimine magnetic beads activates long-term immune responses in mice

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Xing-Bo Liu ◽  
Guo-Wei Yu ◽  
Xin-Yu Gao ◽  
Jin-Long Huang ◽  
Li-Ting Qin ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Plasmid Dna ◽  
Magnetic Beads ◽  
Vaccine Development ◽  
Bovine Herpesvirus ◽  
Intranasal Route ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

Abstract Background DNA vaccine is one of the research hotspots in veterinary vaccine development. Several advantages, such as cost-effectiveness, ease of design and production, good biocompatibility of plasmid DNA, attractive biosafety, and DNA stability, are found in DNA vaccines. Methods In this study, the plasmids expressing bovine herpesvirus 1 (BoHV-1) gB, gC, and gD proteins were mixed at the same mass ratio and adsorbed polyethyleneimine (PEI) magnetic beads with a diameter of 50 nm. Further, the plasmid and PEI magnetic bead polymers were packaged into double carboxyl polyethylene glycol (PEG) 600 to use as a DNA vaccine. The prepared DNA vaccine was employed to vaccinate mice via the intranasal route. The immune responses were evaluated in mice after vaccination. Results The expression of viral proteins could be largely detected in the lung and rarely in the spleen of mice subjected to a vaccination. The examination of biochemical indicators, anal temperature, and histology indicated that the DNA vaccine was safe in vivo. However, short-time toxicity was observed. The total antibody detected with ELISA in vaccinated mice showed a higher level than PBS, DNA, PEI + DNA, and PBS groups. The antibody level was significantly elevated at the 15th week and started to decrease since the 17th week. The neutralizing antibody titer was significantly higher in DNA vaccine than naked DNA vaccinated animals. The total IgA level was much greater in the DNA vaccine group compared to other component vaccinated groups. The examination of cellular cytokines and the percentage of CD4/CD8 indicated that the prepared DNA vaccine induced a strong cellular immunity. Conclusion The mixed application of plasmids expressing BoHV-1 gB/gC/gD proteins by nano-carrier through intranasal route could effectively activate long-term humoral, cellular, and mucosal immune responses at high levels in mice. These data indicate PEI magnetic beads combining with PEG600 are an efficient vector for plasmid DNA to deliver intranasally as a DNA vaccine candidate.

scholarly journals Intradermal immunization with a bovine herpesvirus-1 DNA vaccine induces protective immunity in cattle.

1998 ◽  
Vol 79 (4) ◽  
pp. 831-839 ◽  
Author(s):  
van Drunen Littel-van den Hurk ◽  
B C Karvonen ◽  
L A Babiuk ◽  
P J Lewis ◽  
T Watts ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Protective Immunity ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Intradermal Immunization ◽  
Herpesvirus 1

scholarly journals Inclusion of the Bovine Neutrophil Beta-Defensin 3 with Glycoprotein D of Bovine Herpesvirus 1 in a DNA Vaccine Modulates Immune Responses of Mice and Cattle

2014 ◽  
Vol 21 (4) ◽  
pp. 463-477 ◽  
Author(s):  
Sarah Mackenzie-Dyck ◽  
Jennifer Kovacs-Nolan ◽  
Marlene Snider ◽  
Lorne A. Babiuk ◽  
Sylvia van Drunen Littel-van den Hurk
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Protective Antigen ◽  
Bovine Herpesvirus ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Ifn Γ ◽  
Herpesvirus 1 ◽  
Antibody Levels ◽  
Bovine Neutrophil

ABSTRACTBovine herpesvirus 1 (BoHV-1) causes recurrent respiratory and genital infections in cattle and predisposes them to lethal secondary infections. While modified live and killed BoHV-1 vaccines exist, these are not without problems. Development of an effective DNA vaccine for BoHV-1 has the potential to address these issues. As a strategy to enhance DNA vaccine immunity, a plasmid encoding the bovine neutrophil beta-defensin 3 (BNBD3) as a fusion with truncated glycoprotein D (tgD) and a mix of two plasmids encoding BNBD3 and tgD were tested in mice and cattle. In mice, coadministration of BNBD3 on the separate plasmid enhanced the tgD-induced gamma interferon (IFN-γ) response but not the antibody response. BNBD3 fused to tgD did not affect the antibody levels or the number of IFN-γ-secreting cells but increased the induction of tgD-specific cytotoxic T lymphocytes (CTLs). In cattle, the addition of BNBD3 as a fusion construct also modified the immune response. While the IgG and virus-neutralizing antibody levels were not affected, the number of IFN-γ-secreting cells was increased after BoHV-1 challenge, specifically the CD8+IFN-γ+T cells, including CD8+IFN-γ+CD25+CTLs. While reduced virus shedding, rectal temperature, and weight loss were observed, the level of protection was comparable to that observed in pMASIA-tgD-vaccinated animals. These data show that coadministration of BNBD3 with a protective antigen as a fusion in a DNA vaccine strengthened the Th1 bias and increased cell-mediated immune responses but did not enhance protection from BoHV-1 infection.

scholarly journals Immunogenicity of a Bovine Herpesvirus 1 Glycoprotein D DNA Vaccine Complexed with Bovine Neutrophil Beta-Defensin 3

2014 ◽  
Vol 22 (1) ◽  
pp. 79-90 ◽  
Author(s):  
Sarah Mackenzie-Dyck ◽  
Laura Latimer ◽  
Ethel Atanley ◽  
Jennifer Kovacs-Nolan ◽  
Sam Attah-Poku ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Protective Efficacy ◽  
Bovine Herpesvirus ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1 ◽  
Humoral Responses ◽  
Bovine Neutrophil

ABSTRACTProtective efficacy against bovine herpesvirus 1 (BoHV-1) has been demonstrated to be induced by a plasmid encoding bovine neutrophil beta-defensin 3 (BNBD3) as a fusion construct with truncated glycoprotein D (tgD). However, in spite of the increased cell-mediated immune responses induced by this DNA vaccine, the clinical responses of BoHV-1-challenged cattle were not reduced over those observed in animals vaccinated with the plasmid encoding tgD alone; this might have been because the vaccine failed to improve humoral responses. We hypothesized that an alternative vaccine design strategy that utilized the DNA vaccine pMASIA-tgD as a complex with BNBD3 might improve humoral responses while maintaining robust Th1-type cell-mediated responses. C57BL/6 mice were vaccinated with pMASIA-tgD complexed with 0, 0.01875, 0.1875, or 1.875 nmol of a stable synthesized analog of BNBD3 (aBNBD3). The best results were seen in mice immunized with the vaccine composed of pMASIA-tgD complexed to 0.1875 nmol aBNBD3. In this group, humoral responses were improved, as evidenced by increased virus neutralization, tgD-specific early IgG1, and later IgG2a titers, while the strong cell-mediated immune responses, measured based on specific gamma interferon (IFN-γ)-secreting cells, were maintained relative to pMASIA-tgD. Modulation of the immune response might have been due in part to the effect of BNBD3 on dendritic cells (DCs).In vitrostudies showed that murine bone marrow-derived DCs (BMDCs) pretreated with aBNBD3 were activated, as evidenced by CD11c downregulation, and were functionally mature, as shown by increased allostimulatory ability. Native, synthetic, and analog forms of BNBD3 were equally capable of inducing functional maturation of BMDCs.

Use of Adjuvants to Enhance the Immune Response Induced by a DNA Vaccine Against Bovine Herpesvirus-1

2015 ◽  
Vol 28 (6) ◽  
pp. 343-346 ◽  
Author(s):  
Sebastián Di Giacomo ◽  
Valeria Quattrocchi ◽  
Patricia Zamorano
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1
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