Cytomegalovirus Upregulates Vascular Endothelial Growth Factor and Its Second Cellular Kinase Domain Receptor in Human Fibroblasts

2012 ◽  
Vol 25 (5) ◽  
pp. 360-367 ◽  
Author(s):  
Andreas Heiske ◽  
Yvonne Roettger ◽  
Michael Bacher
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Human Fibroblasts ◽  
Kinase Domain ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

scholarly journals Crystal structure of the kinase domain of human vascular endothelial growth factor receptor 2: a key enzyme in angiogenesis

Structure ◽  
1999 ◽  
Vol 7 (3) ◽  
pp. 319-330 ◽  
Author(s):  
Michele A McTigue ◽  
John A Wickersham ◽  
Chris Pinko ◽  
Richard E Showalter ◽  
Camran V Parast ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Growth Factor Receptor ◽  
Kinase Domain ◽  
Vascular Endothelial ◽  
Key Enzyme ◽  
Endothelial Growth Factor

scholarly journals Tissue Factor-Dependent Vascular Endothelial Growth Factor Production by Human Fibroblasts in Response to Activated Factor VII

Blood ◽  
1998 ◽  
Vol 91 (8) ◽  
pp. 2698-2703 ◽  
Author(s):  
Véronique Ollivier ◽  
Stéphane Bentolila ◽  
Jacques Chabbat ◽  
Jacques Hakim ◽  
Dominique de Prost
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Tissue Factor ◽  
Human Fibroblasts ◽  
Cell Surface Receptor ◽  
Factor Vii ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vascular Endothelial ◽  
Activated Factor Vii ◽  
Endothelial Growth Factor

The transmembrane protein tissue factor (TF) is the cell surface receptor for coagulation factor VII (FVII) and activated factor VII (FVIIa). Recently, TF has been identified as a regulator of angiogenesis, tumor growth, and metastasis. This study was designed to link the binding of FVII(a) to its receptor, TF, with the subsequent triggering of angiogenesis through vascular endothelial growth factor (VEGF) production by human lung fibroblasts. We report that incubation of fibroblasts, which express constitutive surface TF, with FVII(a) induces VEGF synthesis. FVII(a)-induced VEGF secretion, assessed by a specific enzyme-linked immunosorbent assay, was time- and concentration-dependent. VEGF secretion was maximal after 24 hours of incubation of the cells with 100 nmol/L FVII(a) and represented a threefold induction of the basal VEGF level. Reverse transcriptase-polymerase chain reaction analysis of VEGF detected three mRNA species of 180, 312, and 384 bp corresponding, respectively, to VEGF121, VEGF165, and VEGF189. A 2.5- to 3.5-fold increase was observed for the 180- and 312-bp transcripts at 12 and 24 hours, respectively. FVII(a)-dependent VEGF production was inhibited by a pool of antibodies against TF, pointing to the involvement of this receptor. On specific active-site inhibition with dansyl-glutamyl-glycinyl-arginyl chloromethyl ketone, FVIIa lost 70% of its capacity to elicit VEGF production. Consistent with this, the native form (zymogen) of FVII only had a 1.8-fold stimulating effect. Protein tyrosine kinase and protein kinase C are involved in signal transduction leading to VEGF production, as shown by the inhibitory effects of genistein and GF 109203X. The results of this study indicate that TF is essential for VIIa-induced VEGF production by human fibroblasts and that its role is mainly linked to the proteolytic activity of the TF-VIIa complex.

Modulation of the expression of vascular endothelial growth factor in human fibroblasts

2005 ◽  
Vol 83 (2) ◽  
pp. 405-409 ◽  
Author(s):  
Michael P. Diamond ◽  
Eslam El-Hammady ◽  
Adnan Munkarah ◽  
Eric J. Bieber ◽  
Ghassan Saed
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Human Fibroblasts ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

The impact of clay-based hypoxia mimetic hydrogel on human fibroblasts of the periodontal soft tissue

2019 ◽  
Vol 33 (9) ◽  
pp. 1277-1284 ◽  
Author(s):  
Anna Sonja Müller ◽  
Milot Gashi ◽  
Klara Janjić ◽  
Michael Edelmayer ◽  
Andreas Moritz ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Soft Tissue ◽  
Culture Medium ◽  
Human Fibroblasts ◽  
Vascular Endothelial ◽  
Growth Factor Production ◽  
Endothelial Growth Factor ◽  
The Impact ◽  
Synthetic Clay

Thixotropic clays have favorable properties for tissue regeneration. Hypoxia mimetic agents showed promising results in pre-clinical models for hard and soft tissue regeneration. It is unclear if clays can be used as carrier for hypoxia mimetic agent in a periodontal regenerative setting. Here, we tested the response of human fibroblasts of the periodontal soft tissue to synthetic clay hydrogels and assessed hypoxia mimetic agent release. Cells were cultured on synthetic clay hydrogels (5.00%–0.15%). We assessed viability and differentiation capacity with resazurin-based toxicity assays, MTT staining, Live-Dead staining, and alkaline phosphatase staining. To reveal the response of fibroblasts to hypoxia mimetic agent-loaded clay hydrogels, cells were exposed to clay supplemented with dimethyloxalylglycine, deferoxamine, l-mimosine, and CoCl2. Supernatants from hypoxia mimetic agent-loaded clay hydrogels were harvested and replaced with medium at hour 1, 3, 6, 24, 48, and 72. To reveal the hypoxia mimetic capacity of supernatants, vascular endothelial growth factor production in the fibroblasts was assessed in the culture medium. Our data show that clay did not induce relevant toxic effects in the fibroblasts which remained capable to differentiate into alkaline phosphatase-positive cells at the relevant concentrations. Fibroblasts cultured on clay hydrogel loaded with dimethyloxalylglycine, deferoxamine, l-mimosine, and CoCl2 remained vital, however, no significant increase in vascular endothelial growth factor levels was found in the culture medium. Only dimethyloxalylglycine-loaded clay supernatants taken in the first hours stimulated vascular endothelial growth factor production in fibroblasts. In conclusion no pronounced toxic effects of synthetic clay were observed. Supplementation with dimethyloxalylglycine leads to hypoxia mimetic activity. This pilot study provides first insights into the impact of synthetic clay on periodontal tissue.

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