Molecular Detection and Genetic Diversity of Leishmania donovani in Naturally Infected Phlebotomus chinensi from Southwestern China

2011 ◽  
Vol 11 (7) ◽  
pp. 849-852 ◽  
Author(s):  
Feng Wei ◽  
Limin Shang ◽  
Hongtao Jin ◽  
Hai Lian ◽  
Wensen Liu ◽  
...  
2010 ◽  
Vol 125 (4) ◽  
pp. 389-393 ◽  
Author(s):  
S.H. Hamad ◽  
E.A.G. Khalil ◽  
A.M. Musa ◽  
M.E. Ibrahim ◽  
B.M. Younis ◽  
...  

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Yu Deng ◽  
Silu Wang ◽  
Runxia Liu ◽  
Guiying Hao

Bovine viral diarrhea virus (BVDV) affects cows, pigs, sheep, goats, and other ruminants, as well as some wild animals. BVDV causes considerable economic losses every year and many countries have developed programs aimed at the eradication of this disease. The genetic diversity of BVDV in diseased goats has never been described in southwestern China. Thus, in this study, we applied antigen-capture ELISA and RT-PCR to survey the infection rate of BVDV in diseased goats in this region. Our results demonstrated that the average BVDV infection rate in goats was 17.51%, with all positive samples indicating infection by BVDV-1 and not BVDV-2, BVDV-3, or Border disease virus. The molecular characteristics of the 5′-untranslated region (5′-UTR) of BVDV-1 were recognized as belonging predominantly to the BVDV-1a, 1b, 1c, 1m, and 1p subtypes. BVDV-1b and 1m were the most abundant subtypes identified in this region, similar to the BVDV epidemics in cattle in other regions of China. This is the first study that describes the genetic characterization of BVDV in sick goats from southwestern China and is important for future studies and control programs.


2018 ◽  
Vol 18 (3) ◽  
Author(s):  
Rong Wang ◽  
Zhihan Zhang ◽  
Xia Hu ◽  
Songqing Wu ◽  
Jinda Wang ◽  
...  

2010 ◽  
Vol 155 (4) ◽  
pp. 589-593 ◽  
Author(s):  
Giuseppina La Rosa ◽  
Marcello Iaconelli ◽  
Manoochehr Pourshaban ◽  
Marta Fratini ◽  
Michele Muscillo

2004 ◽  
Vol 32 (9) ◽  
pp. 797-807 ◽  
Author(s):  
De-Lian Wang ◽  
Zhong-Chao Li ◽  
Gang Hao ◽  
Tzen-Yuh Chiang ◽  
Xue-Jun Ge

2015 ◽  
Vol 31 ◽  
pp. 53-60 ◽  
Author(s):  
Masashi Terao ◽  
Shirin Akter ◽  
Md. Golam Yasin ◽  
Ryo Nakao ◽  
Hirotomo Kato ◽  
...  

1998 ◽  
Vol 06 (2) ◽  
pp. 143-150
Author(s):  
QIU Fang ◽  
JIN De-Min ◽  
WANG Bin ◽  
FU Jian-Min ◽  

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuliang Jiang ◽  
Tsam Ju ◽  
Linda E. Neaves ◽  
Jialiang Li ◽  
Weining Tan ◽  
...  

Population genetic assessment is crucial for the conservation and management of threatened species. Xanthocyparis vietnamensis is an endangered species that is currently restricted to karst mountains in southwestern China and Vietnam. This rare conifer was first recorded in 2002 from northern Vietnam and then in 2013 from Guangxi, China, yet nothing is known about its genetic diversity nor ploidy level variation, although previous cytological study suggest that Vietnamese populations are tetraploids. There have been about 45 individuals found to date in Guangxi, China. Here, we genotyped 33 X. vietnamensis individuals using 20 newly developed, polymorphic microsatellite loci, to assess the genetic variability of its extremely small populations. The genetic diversity of X. vietnamensis (HE = 0.511) was lower than that of two other heliophile species, Calocedrus macrolepis and Fokienia hodginsii, which have similar distribution ranges. This is consistent with the signature of a genetic bottleneck detected in X. vietnamensis. Although the population genetic differentiation coefficient across loci is moderate (FST = 0.125), STRUCTURE analysis revealed two distinct genetic clusters, namely the northern and southern population groups; DAPC analysis grouped the southern populations together in one cluster separate from the northern populations; AMOVA analysis detected a significant genetic differentiation between the two population groups (FRT = 0.089, p < 0.05), and BARRIER analysis detected a genetic barrier between them. Moreover, we detected differentiation in ploidy level between northern and southern populations, sampled individuals from the former and the later are all diploid and tetraploid cytotypes with mean genome sizes of 26.08 and 48.02 pg/2C, respectively. We deduced that heterogeneous geomorphology and historical events (e.g., human deforestation, Quaternary climate oscillations) may have contributed to population fragmentation and small population size in X. vietnamensis. Considering both genetic and ploidy level differentiation, we propose that two different management units (northern and southern) should be considered and a combination of in situ and ex situ conservation measures should be employed to preserve populations of this endangered species in southwestern China in the light of our findings.


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