Detection of Usutu Virus Within a West Nile Virus Surveillance Program in Northern Italy

2011 ◽  
Vol 11 (5) ◽  
pp. 551-557 ◽  
Author(s):  
Marco Tamba ◽  
Paolo Bonilauri ◽  
Romeo Bellini ◽  
Mattia Calzolari ◽  
Alessandro Albieri ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Northern Italy ◽  
Surveillance Program ◽  
West Nile ◽  
Usutu Virus ◽  
Virus Surveillance

scholarly journals Seasonal Phenological Patterns and Flavivirus Vectorial Capacity of Medically Important Mosquito Species in a Wetland and an Urban Area of Attica, Greece

2021 ◽  
Vol 6 (4) ◽  
pp. 176
Author(s):  
Stavroula Beleri ◽  
Georgios Balatsos ◽  
Vasilios Karras ◽  
Nikolaos Tegos ◽  
Fani Sereti ◽  
...  
Keyword(s):  
Public Health ◽  
West Nile Virus ◽  
Mosquito Species ◽  
Vectorial Capacity ◽  
Adult Mosquito ◽  
Surveillance Program ◽  
The South ◽  
Public Health Importance ◽  
West Nile ◽  
Usutu Virus

Seasonal patterns of mosquito population density and their vectorial capacity constitute major elements to understand the epidemiology of mosquito-borne diseases. Using adult mosquito traps, we compared the population dynamics of major mosquito species (Culex pipiens, Aedes albopictus, Anopheles spp.) in an urban and a wetland rural area of Attica Greece. Pools of the captured Cx. pipiens were analyzed to determine infection rates of the West Nile virus (WNV) and the Usutu virus (USUV). The data provided were collected under the frame of the surveillance program carried out in two regional units (RUs) of the Attica region (East Attica and South Sector of Attica), during the period 2017–2018. The entomological surveillance of adult mosquitoes was performed on a weekly basis using a network of BG-sentinel traps (BGs), baited with CO2 and BG-Lure, in selected, fixed sampling sites. A total of 46,726 adult mosquitoes were collected, with larger variety and number of species in East Attica (n = 37,810), followed by the South Sector of Attica (n = 8916). The collected mosquitoes were morphologically identified to species level and evaluated for their public health importance. Collected Cx. pipiens adults were pooled and tested for West Nile virus (WNV) and Usutu virus (USUV) presence by implementation of a targeted molecular methodology (real-time PCR). A total of 366 mosquito pools were analyzed for WNV and USUV, respectively, and 38 (10.4%) positive samples were recorded for WNV, while no positive pool was detected for USUV. The majority of positive samples for WNV were detected in the East Attica region, followed by the South Sector of Attica, respectively. The findings of the current study highlight the WNV circulation in the region of Attica and the concomitant risk for the country, rendering mosquito surveillance actions and integrated mosquito management programs as imperative public health interventions.

scholarly journals West Nile Virus Surveillance in 2013 via Mosquito Screening in Northern Italy and the Influence of Weather on Virus Circulation

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0140915 ◽  
Author(s):  
Mattia Calzolari ◽  
Alessandra Pautasso ◽  
Fabrizio Montarsi ◽  
Alessandro Albieri ◽  
Romeo Bellini ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Northern Italy ◽  
West Nile ◽  
Virus Surveillance

scholarly journals West Nile Virus Surveillance and Diagnostic: A Canadian Perspective

2003 ◽  
Vol 14 (2) ◽  
pp. 105-114 ◽  
Author(s):  
Michael A Drebot ◽  
Robbin Lindsay ◽  
Ian K Barker ◽  
Peter A Buck ◽  
Margaret Fearon ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Late Summer ◽  
Surveillance Program ◽  
Prevention Measures ◽  
Genomic Amplification ◽  
West Nile ◽  
Virus Surveillance ◽  
Canadian Perspective ◽  
Human Illness ◽  
Ongoing Surveillance

A surveillance program has been in place since 2000 to detect the presence of West Nile virus (WNV) in Canada. Serological assays are most appropriate when monitoring for human disease and undertaking case investigations. Genomic amplification procedures are more commonly used for testing animal and mosquito specimens collected as part of ongoing surveillance efforts. The incursion of WNV into this country was documented for the first time in 2001 when WNV was demonstrated in 12 Ontario health units during the late summer and fall. In 2002 WNV activity was documented by avian surveillance in Ontario by mid-May with subsequent expansion of the virus throughout Ontario and into Quebec, Manitoba, Saskatchewan and Nova Scotia. Human cases were recorded in both Ontario and Quebec in 2002 with approximately 800 to 1000 probable, confirmed and suspect cases detected. The possible recurrence and further spread of WNV to other parts of Canada in 2003 must be anticipated with potential risk to public health. The continued surveillance and monitoring for WNV-associated human illness is necessary and appropriate disease prevention measures need to be in place in 2003.

scholarly journals West Nile virus infection in individuals with pre-existing Usutu virus immunity, northern Italy, 2018

2019 ◽  
Vol 24 (21) ◽  
Author(s):  
Alessandro Sinigaglia ◽  
Monia Pacenti ◽  
Thomas Martello ◽  
Silvana Pagni ◽  
Elisa Franchin ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Virus Infection ◽  
West Nile Virus Infection ◽  
Northern Italy ◽  
Cross Protection ◽  
West Nile ◽  
Usutu Virus ◽  
Virus Immunity

In 2018, there was a large West Nile virus (WNV) outbreak in northern Italy. We observed five atypical cases of WNV infection that were characterised by the presence of WNV RNA and WNV IgG at the time of diagnosis, but no IgM response during follow-up. Neutralisation assays demonstrated pre-existing Usutu virus immunity in all patients. Besides challenging diagnosis, the immunological crosstalk between the two viruses warrants further investigation on possible cross-protection or infection enhancement effects.

West Nile Virus Surveillance in the Lombardy Region, Northern Italy

2015 ◽  
Vol 62 (4) ◽  
pp. 343-349 ◽  
Author(s):  
M. Chiari ◽  
A. Prosperi ◽  
F. Faccin ◽  
D. Avisani ◽  
M. Cerioli ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Northern Italy ◽  
West Nile ◽  
Lombardy Region ◽  
Virus Surveillance

scholarly journals Co‐Infections: Simultaneous Detections of West Nile Virus and Usutu Virus in Birds from Germany

2021 ◽  
Author(s):  
Pauline Dianne Santos ◽  
Friederike Michel ◽  
Claudia Wylezich ◽  
Dirk Höper ◽  
Markus Keller ◽  
...  
Keyword(s):  
West Nile Virus ◽  
West Nile ◽  
Usutu Virus
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