scholarly journals The Chorioallantoic Membrane Assay for Biomaterial Testing in Tissue Engineering: A Short-TermIn VivoPreclinical Model

2017 ◽  
Vol 23 (12) ◽  
pp. 938-952 ◽  
Author(s):  
Inés Moreno-Jiménez ◽  
Janos M. Kanczler ◽  
Gry Hulsart-Billstrom ◽  
Stefanie Inglis ◽  
Richard O.C. Oreffo
Keyword(s):  
Tissue Engineering ◽  
Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay

scholarly journals Evolving applications of the egg: chorioallantoic membrane assay and ex vivo organotypic culture of materials for bone tissue engineering

2020 ◽  
Vol 11 ◽  
pp. 204173142094273
Author(s):  
Karen M Marshall ◽  
Janos M Kanczler ◽  
Richard OC Oreffo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Organotypic Culture ◽  
Ex Vivo ◽  
Chorioallantoic Membrane ◽  
Experimental Methodology ◽  
Chick Chorioallantoic Membrane ◽  
Recent Developments ◽  
Chorioallantoic Membrane Assay

The chick chorioallantoic membrane model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the chorioallantoic membrane assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the chorioallantoic membrane assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches, including timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo chorioallantoic membrane assay and the ex vivo organotypic culture to advance the field of bone tissue engineering, and highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

scholarly journals Chorioallantoic Membrane Assay as Model for Angiogenesis in Tissue Engineering: Focus on Stem Cells

2020 ◽  
Author(s):  
Greet Merckx ◽  
Hanna Tay ◽  
Melissa Lo Monaco ◽  
Marc van Zandvoort ◽  
Ward De Spiegelaere ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Chorioallantoic Membrane ◽  
Chorioallantoic Membrane Assay

scholarly journals Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

2021 ◽  
Vol 12 ◽  
pp. 204173142110056
Author(s):  
Nupur Kohli ◽  
Vaibhav Sharma ◽  
Alodia Orera ◽  
Prasad Sawadkar ◽  
Nazanin Owji ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Clinical Model ◽  
Chick Chorioallantoic Membrane ◽  
Biomimetic Method ◽  
Alginate Scaffold

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

scholarly journals Patient Derived Chicken Egg Tumor Model (PDcE Model): Current Status and Critical Issues

Cells ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 440 ◽  
Author(s):  
Aoi Komatsu ◽  
Kotaro Matsumoto ◽  
Tomoki Saito ◽  
Manabu Muto ◽  
Fuyuhiko Tamanoi
Keyword(s):  
Cancer Patients ◽  
Chorioallantoic Membrane ◽  
Tumor Model ◽  
Tumor Formation ◽  
Current Status ◽  
Cam Assay ◽  
Chicken Egg ◽  
Critical Issues ◽  
Chicken Eggs ◽  
Chorioallantoic Membrane Assay

Chorioallantoic membrane assay (CAM assay) using fertilized chicken eggs has been used for the study of tumor formation, angiogenesis and metastasis. Recently, there is growing realization that this system provides a valuable assay for a patient-derived tumor model. Several reports establish that tumor samples from cancer patients can be used to reproduce tumor in the chicken egg. High transplantation efficiency has been achieved. In this review, we discuss examples of transplanting patient tumors. We then discuss critical issues that need to be addressed to pursue this line of experiments. The patient-derived chicken egg model (PDcE model) has an advantage over other models in its rapid tumor formation. This raises the possibility that the PDcE model is valuable for identifying optimum drug for each individual patient.

scholarly journals Angiogenesis and Diabetes: Different Responses to Pro-Angiogenic Factors in the Chorioallantoic Membrane Assay

2008 ◽  
Vol 14 (11-12) ◽  
pp. 705-714
Author(s):  
Giovana S. Di Marco ◽  
Antoine Alam ◽  
Frédéric Dol ◽  
Pierre Corvol ◽  
Jean-Marie Gasc ◽  
...  
Keyword(s):  
Chorioallantoic Membrane ◽  
Angiogenic Factors ◽  
Chorioallantoic Membrane Assay

scholarly journals Chick Chorioallantoic Membrane Assay: A 3D Animal Model for Study of Human Nasopharyngeal Carcinoma

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0130935 ◽  
Author(s):  
Xue Xiao ◽  
Xiaoying Zhou ◽  
Huixin Ming ◽  
Jinyan Zhang ◽  
Guangwu Huang ◽  
...  
Keyword(s):  
Animal Model ◽  
Nasopharyngeal Carcinoma ◽  
Chorioallantoic Membrane ◽  
Chick Chorioallantoic Membrane ◽  
Human Nasopharyngeal Carcinoma ◽  
Chorioallantoic Membrane Assay
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