Superior Red Blood Cell Generation from Human Pluripotent Stem Cells Through a Novel Microcarrier-Based Embryoid Body Platform

2016 ◽  
Vol 22 (8) ◽  
pp. 765-780 ◽  
Author(s):  
Jaichandran Sivalingam ◽  
Alan Tin-Lun Lam ◽  
Hong Yu Chen ◽  
Bin Xia Yang ◽  
Allen Kuan-Liang Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Embryoid Body ◽  
Human Pluripotent Stem Cells ◽  
Cell Generation

Superior Red Blood Cell Generation From Human Pluripotent Stem Cells via a Novel Microcarrier Based Embryoid Body Platform

Cytotherapy ◽  
2016 ◽  
Vol 18 (6) ◽  
pp. S41-S42
Author(s):  
J. Sivalingam ◽  
E. Lee ◽  
A. Lam ◽  
A. Chen ◽  
S. Reuveny ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Embryoid Body ◽  
Human Pluripotent Stem Cells ◽  
Cell Generation

Red blood cell generation from human induced pluripotent stem cells of different donor cell type origin

2013 ◽  
Vol 41 (8) ◽  
pp. S27
Author(s):  
Isabel Dorn ◽  
Katharina Klich ◽  
Martina Radstaak ◽  
Katherina Psathaki ◽  
Marcos Arauzo-Bravo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Induced Pluripotent Stem Cells ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Donor Cell ◽  
Cell Generation ◽  
Cell Type ◽  
Induced Pluripotent ◽  
Donor Cell Type

scholarly journals Red blood cell generation from human induced pluripotent stem cells: perspectives for transfusion medicine

Haematologica ◽  
2010 ◽  
Vol 95 (10) ◽  
pp. 1651-1659 ◽  
Author(s):  
H. Lapillonne ◽  
L. Kobari ◽  
C. Mazurier ◽  
P. Tropel ◽  
M.-C. Giarratana ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Induced Pluripotent Stem Cells ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Cell Generation ◽  
Transfusion Medicine ◽  
Induced Pluripotent

scholarly journals PS1235 RED BLOOD CELL GENERATION FROM HUMAN INDUCED PLURIPOTENT STEM CELLS

HemaSphere ◽  
2019 ◽  
Vol 3 (S1) ◽  
pp. 562
Author(s):  
E. Varga ◽  
M. Hansen ◽  
S. Heshusius ◽  
P. Burger ◽  
T. Wust ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Induced Pluripotent Stem Cells ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Cell Generation ◽  
Induced Pluripotent

Differentiation of Human Pluripotent Stem Cells for Red Blood Cell Production

2018 ◽  
pp. 47-62
Author(s):  
Mark C. Allenby ◽  
Susana Brito dos Santos ◽  
Nicki Panoskaltsis ◽  
Athanasios Mantalaris
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Cell Production

scholarly journals Study of RBC Histograms and Its Correlation with Etiopathogenesis and Other Parameters in Various Anemias

2021 ◽  
pp. 66-82
Author(s):  
A. P. Gokula Kannan ◽  
R. Govindarajan ◽  
J. Thanka
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Morphological Features ◽  
Peripheral Smear ◽  
Smear Examination ◽  
Cytoplasmic Rna ◽  
Different Types

The pluripotent stem cells which are present in the bone marrow renew by its own and differentiate into mature cells. These stem cells undergo division by the presence of erythropoietin, where the nucleus is extruded out from the cell during the end of differentiation, thereby retaining cytoplasmic RNA to form a reticulocyte. The reticulocyte i s a precursor to red blood cell and on losing the RNA it matures into a Red Blood Cell. The present study aimed to analyze the correlation between the automated histogram patterns along with morphological features of RBC‘s prepared from peripheral smear examination in different types of anemia. viz., MCV, MCH, MCHC & RWD- CV.

scholarly journals Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 710 ◽  
Author(s):  
Manale Karam ◽  
Ihab Younis ◽  
Noor R. Elareer ◽  
Sara Nasser ◽  
Essam M. Abdelalim
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Pluripotent Stem Cells ◽  
Disease Modeling ◽  
Embryoid Body ◽  
Fold Increase ◽  
Human Pluripotent Stem Cells ◽  
Short Exposure ◽  
Differentiation Potential ◽  
All Trans Retinoic Acid

Human pluripotent stem cells (hPSCs) can provide unlimited supply for mesenchymal stem cells (MSCs) and adipocytes that can be used for therapeutic applications. Here we developed a simple and highly efficient all-trans-retinoic acid (RA)-based method for generating an off-the-shelf and scalable number of human pluripotent stem cell (hPSC)-derived MSCs with enhanced adipogenic potential. We showed that short exposure of multiple hPSC lines (hESCs/hiPSCs) to 10 μM RA dramatically enhances embryoid body (EB) formation through regulation of genes activating signaling pathways associated with cell proliferation, survival and adhesion, among others. Disruption of cell adhesion induced the subsequent differentiation of the highly expanded RA-derived EB-forming cells into a pure population of multipotent MSCs (up to 1542-fold increase in comparison to RA-untreated counterparts). Interestingly, the RA-derived MSCs displayed enhanced differentiation potential into adipocytes. Thus, these findings present a novel RA-based approach for providing an unlimited source of MSCs and adipocytes that can be used for regenerative medicine, drug screening and disease modeling applications.

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