Evidence for Active Electrolyte Transport by Two-Dimensional Monolayers of Human Salivary Epithelial Cells

2015 ◽  
Vol 21 (12) ◽  
pp. 1226-1236 ◽  
Author(s):  
Orsolya Hegyesi ◽  
Anna Földes ◽  
Erzsébet Bori ◽  
Zsolt Németh ◽  
József Barabás ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Electrolyte Transport ◽  
Two Dimensional ◽  
Salivary Epithelial Cells

Evidence for active electrolyte transport of two-dimensional monolayer of human epithelial cells

Pancreatology ◽  
2013 ◽  
Vol 13 (3) ◽  
pp. S30
Author(s):  
Orsolya Hegyesi ◽  
Anna Foldes ◽  
Erzsebet Bori ◽  
Balint Trimmel ◽  
Martin C. Steward ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Electrolyte Transport ◽  
Two Dimensional ◽  
Human Epithelial Cells

278: Gene Expression Profiling of Prostatic Epithelial Cells in Two-Dimensional Versus Three-Dimensional Cultures

2007 ◽  
Vol 177 (4S) ◽  
pp. 93-93
Author(s):  
Toshiyuki Tsunoda ◽  
Junichi Inocuchi ◽  
Darren Tyson ◽  
Seiji Naito ◽  
David K. Ornstein
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Three Dimensional ◽  
Two Dimensional

scholarly journals SUMOylation Attenuates Sensitivity toward Hypoxia- or Desferroxamine-Induced Injury by Modulating Adaptive Responses in Salivary Epithelial Cells

2006 ◽  
Vol 168 (5) ◽  
pp. 1452-1463 ◽  
Author(s):  
Ha-Van Nguyen ◽  
Jo-Lin Chen ◽  
Jenny Zhong ◽  
Kwang-Jin Kim ◽  
Edward D. Crandall ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Adaptive Responses ◽  
Salivary Epithelial Cells

scholarly journals P2X7 Receptor-mediated Membrane Blebbing in Salivary Epithelial Cells

2009 ◽  
Vol 13 (3) ◽  
pp. 175 ◽  
Author(s):  
Sung-Min Hwang ◽  
Na-Youn Koo ◽  
Se-Young Choi ◽  
Gae-Sig Chun ◽  
Joong-Soo Kim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
P2x7 Receptor ◽  
Membrane Blebbing ◽  
Salivary Epithelial Cells

Evidence for the presence of a Hg-inhibitable water-permeability pathway and aquaporin 1 in A5 salivary epithelial cells

1995 ◽  
Vol 164 (3) ◽  
pp. 613-619 ◽  
Author(s):  
Krzysztof W. Lazowski ◽  
Jun Li ◽  
Christine Delporte ◽  
Bruce J. Baum
Keyword(s):  
Epithelial Cells ◽  
Water Permeability ◽  
Aquaporin 1 ◽  
Salivary Epithelial Cells

Evaluation of Water and Electrolyte Transport of Tubular Epithelial Cells Under Osmotic and Hydraulic Pressure for Development of Bioartificial Tubules

2001 ◽  
Vol 25 (3) ◽  
pp. 209-212 ◽  
Author(s):  
Masuo Terashima ◽  
Yuji Fujita ◽  
Katsuhiro Sugano ◽  
Manabu Asano ◽  
Naoko Kagiwada ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Electrolyte Transport ◽  
Hydraulic Pressure ◽  
Tubular Epithelial Cells

The Preparation of Silk Fibroin Modified PBT-co-PBS/PEG Composite Films and their Effects on Clinical Human Salivary Epithelial Cells Transplantation

2013 ◽  
Vol 18 ◽  
pp. 29-42
Author(s):  
Jie Zhu ◽  
Yue Ming Zhang ◽  
Zhi Cheng Tao ◽  
Nan Wei Xu ◽  
Li Qun Wang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Plasma Treatment ◽  
Silk Fibroin ◽  
High Surface ◽  
Composite Films ◽  
Contact Angles ◽  
Butylene Succinate ◽  
Poly Ethylene ◽  
Surface Modified ◽  
Salivary Epithelial Cells

We reported the preparation of surface modified poly(butylene terephthalate)-co-poly(butylene succinate)-b-poly(ethylene glycol) (i.e. PBT-co-PBS/PEG) films by three methods: silk fibroin coating, SO2 plasma treatment and silk fibroin anchoring. The obtained composite films were named SF/(PBT-co-PBS/PEG), SO2/(PBT-co-PBS/PEG) and SF/SO2/(PBT-co-PBS/PEG), respectively. Their surface properties were characterized by contact angles, surface energies and XPS. The biocompatibility of the films were further evaluated by the morphology, attachment, proliferation and viability of human salivary epithelial cells (HSG cells). Results revealed that SF/SO2/(PBT-co-PBS/PEG) possessed the high surface free energy (59.67 mJ/m2) and could immobilize a great amount of fibroin (SF surface coverage: 26.39 wt%), which attributed to the formation of such polar groups as hydrosulfide group, sulfonic group, carboxyl and carbonyl ones in the process of SO2 plasma treatment. The cell tests suggested that the silk fibroin anchoring could significantly enhance the biocompatibility of PBT-co-PBS/PEG, which implied the potential application of fibroin modified PBT-co-PBS/PEG for clinical HSG cells transplantation in artificial salivary gland constructs.

scholarly journals Regulation of prolactin on electrolyte transport across porcine endometrial epithelial cells

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Chatsri Deachapunya ◽  
Sutthasinee Poonyachoti ◽  
Nateetip Krishnamra
Keyword(s):  
Epithelial Cells ◽  
Electrolyte Transport ◽  
Endometrial Epithelial Cells

Bioelectric response of human nasal epithelial cells to polycationic protein

1996 ◽  
Vol 271 (1) ◽  
pp. L159-L165 ◽  
Author(s):  
M. R. Van Scott ◽  
M. D. Smith ◽  
C. A. Welch ◽  
C. Bentzel ◽  
W. J. Metzger
Keyword(s):  
Epithelial Cells ◽  
Airway Epithelium ◽  
Apical Membrane ◽  
Primary Cultures ◽  
Electrolyte Transport ◽  
Bathing Solution ◽  
Apical Surface ◽  
Nasal Epithelial Cells ◽  
Cl Secretion ◽  
Human Nasal Epithelial Cells

Polycationic proteins alter electrolyte transport by epithelium and endothelium, and in asthma are thought to disrupt the airway epithelium and contribute to hyperresponsiveness and airway plugging. In the present study, we used primary cultures of human nasal epithelial cells to investigate the response of respiratory tract epithelium to luminal presentation of a polycationic protein, protamine. Protamine (100 micrograms/ml) in the apical bathing solution had no significant effect on basal transepithelial resistance (Rt) but decreased short-circuit current (Isc) and hyperpolarized the apical membrane, indicating that Na+ absorption had been inhibited. Pretreating with amiloride inverted the response to protamine, resulting in an increase in Isc, depolarization of the apical membrane, and decrease in the fractional resistance of the apical membrane (fRa). The increase in Isc was inhibited by pretreatment with bumetanide. These results indicated that protamine augmented amiloride-induced Cl- secretion. Induction of Cl- secretion by bathing the apical surface in 3 mM Cl(-)-Ringer solution similarly resulted in protamine-induced depolarization of the apical membrane. Heparin precipitated protamine from solution and reversed the Isc responses. In summary, low concentrations of polycationic protein can alter electrolyte transport by human airway epithelium without desquamation, and the response is dependent on the secretory state of the tissue.

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