scholarly journals Optimized Hyaluronic Acid–Hydrogel Design and Culture Conditions for Preservation of Mesenchymal Stem Cell Properties

2013 ◽  
Vol 19 (4) ◽  
pp. 288-298 ◽  
Author(s):  
Faïza Mohand-Kaci ◽  
Nabila Assoul ◽  
Isabelle Martelly ◽  
Eric Allaire ◽  
Mustapha Zidi
Keyword(s):  
Stem Cell ◽  
Hyaluronic Acid ◽  
Mesenchymal Stem Cell ◽  
Culture Conditions ◽  
Hyaluronic Acid Hydrogel

Modulation of mesenchymal stem cell chondrogenesis in a tunable hyaluronic acid hydrogel microenvironment

Biomaterials ◽  
2012 ◽  
Vol 33 (15) ◽  
pp. 3835-3845 ◽  
Author(s):  
Wei Seong Toh ◽  
Teck Chuan Lim ◽  
Motoichi Kurisawa ◽  
Myron Spector
Keyword(s):  
Stem Cell ◽  
Hyaluronic Acid ◽  
Mesenchymal Stem Cell ◽  
Hyaluronic Acid Hydrogel

scholarly journals A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell‐derived epithelial tissue patches

2019 ◽  
Vol 14 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Pramod Kumar ◽  
Sait Ciftci ◽  
Julien Barthes ◽  
Helena Knopf‐Marques ◽  
Céline Blandine Muller ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hyaluronic Acid ◽  
Mesenchymal Stem Cell ◽  
Epithelial Tissue ◽  
Hyaluronic Acid Hydrogel

scholarly journals Engineering large cartilage tissues using dynamic bioreactor culture at defined oxygen conditions

2018 ◽  
Vol 9 ◽  
pp. 204173141775371 ◽  
Author(s):  
Andrew C Daly ◽  
Binulal N Sathy ◽  
Daniel J Kelly
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Culture Conditions ◽  
Cartilage Tissue ◽  
Bioreactor Culture ◽  
Dynamic Culture ◽  
Oxygen Conditions ◽  
Static Conditions

Mesenchymal stem cells maintained in appropriate culture conditions are capable of producing robust cartilage tissue. However, gradients in nutrient availability that arise during three-dimensional culture can result in the development of spatially inhomogeneous cartilage tissues with core regions devoid of matrix. Previous attempts at developing dynamic culture systems to overcome these limitations have reported suppression of mesenchymal stem cell chondrogenesis compared to static conditions. We hypothesize that by modulating oxygen availability during bioreactor culture, it is possible to engineer cartilage tissues of scale. The objective of this study was to determine whether dynamic bioreactor culture, at defined oxygen conditions, could facilitate the development of large, spatially homogeneous cartilage tissues using mesenchymal stem cell laden hydrogels. A dynamic culture regime was directly compared to static conditions for its capacity to support chondrogenesis of mesenchymal stem cells in both small and large alginate hydrogels. The influence of external oxygen tension on the response to the dynamic culture conditions was explored by performing the experiment at 20% O2 and 3% O2. At 20% O2, dynamic culture significantly suppressed chondrogenesis in engineered tissues of all sizes. In contrast, at 3% O2 dynamic culture significantly enhanced the distribution and amount of cartilage matrix components (sulphated glycosaminoglycan and collagen II) in larger constructs compared to static conditions. Taken together, these results demonstrate that dynamic culture regimes that provide adequate nutrient availability and a low oxygen environment can be employed to engineer large homogeneous cartilage tissues. Such culture systems could facilitate the scaling up of cartilage tissue engineering strategies towards clinically relevant dimensions.

scholarly journals Donor Variation and Optimization of Human Mesenchymal Stem Cell Chondrogenesis in Hyaluronic Acid

2018 ◽  
Vol 24 (21-22) ◽  
pp. 1693-1703 ◽  
Author(s):  
Minwook Kim ◽  
Isaac E. Erickson ◽  
Alice H. Huang ◽  
Sean T. Garrity ◽  
Robert L. Mauck ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hyaluronic Acid ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cell ◽  
Donor Variation
Sign in / Sign up

Export Citation Format

Share Document