scholarly journals Developmental Cues for Bone Formation from Parathyroid Hormone and Parathyroid Hormone-Related Protein in an Ex Vivo Organotypic Culture System of Embryonic Chick Femora

2012 ◽  
Vol 18 (12) ◽  
pp. 984-994 ◽  
Author(s):  
Emma L. Smith ◽  
Janos M. Kanczler ◽  
Carol A. Roberts ◽  
Richard O.C. Oreffo
Keyword(s):  
Parathyroid Hormone ◽  
Bone Formation ◽  
Culture System ◽  
Organotypic Culture ◽  
Ex Vivo ◽  
Embryonic Chick ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein

Parathyroid Hormone-Related Protein Expression and Secretion in a Skin Organotypic Culture System

Endocrine ◽  
1998 ◽  
Vol 8 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Eric A. G. Blomme ◽  
James R. Werkmeister ◽  
Hong Zhou ◽  
Vicky Kartsogiannis ◽  
Charles C. Capen ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Protein Expression ◽  
Culture System ◽  
Organotypic Culture ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein

scholarly journals Increased Potential of Bone Formation with the Intravenous Injection of a Parathyroid Hormone-Related Protein Minicircle DNA Vector

2021 ◽  
Vol 22 (16) ◽  
pp. 9069
Author(s):  
Jang-Woon Kim ◽  
Narae Park ◽  
Jaewoo Kang ◽  
Yena Kim ◽  
Hyerin Jung ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Formation ◽  
Bone Structure ◽  
Human Mesenchymal Stem Cells ◽  
Treated Group ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein ◽  
Alternative Delivery ◽  
Minicircle Dna

Osteoporosis is commonly treated via the long-term usage of anti-osteoporotic agents; however, poor drug compliance and undesirable side effects limit their treatment efficacy. The parathyroid hormone-related protein (PTHrP) is essential for normal bone formation and remodeling; thus, may be used as an anti-osteoporotic agent. Here, we developed a platform for the delivery of a single peptide composed of two regions of the PTHrP protein (1–34 and 107–139); mcPTHrP 1–34+107–139 using a minicircle vector. We also transfected mcPTHrP 1–34+107–139 into human mesenchymal stem cells (MSCs) and generated Thru 1–34+107–139-producing engineered MSCs (eMSCs) as an alternative delivery system. Osteoporosis was induced in 12-week-old C57BL/6 female mice via ovariectomy. The ovariectomized (OVX) mice were then treated with the two systems; (1) mcPTHrP 1–34+107–139 was intravenously administered three times (once per week); (2) eMSCs were intraperitoneally administered twice (on weeks four and six). Compared with the control OVX mice, the mcPTHrP 1–34+107–139-treated group showed better trabecular bone structure quality, increased bone formation, and decreased bone resorption. Similar results were observed in the eMSCs-treated OVX mice. Altogether, these results provide experimental evidence to support the potential of delivering PTHrP 1–34+107–139 using the minicircle technology for the treatment of osteoporosis.

Parathyroid Hormone–Related Protein Inhibits Endothelin-1 Production

Hypertension ◽  
1996 ◽  
Vol 27 (3) ◽  
pp. 360-363 ◽  
Author(s):  
Bingbing Jiang ◽  
Shigeto Morimoto ◽  
Keisuke Fukuo ◽  
Atsushi Hirotani ◽  
Michio Tamatani ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Related Protein ◽  
Endothelin 1 ◽  
Parathyroid Hormone Related Protein
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