Accelerated bone formation and increased osteoblast number contribute to the abnormal tooth germ development in parathyroid hormone-related protein knockout mice

Bone ◽  
2004 ◽  
Vol 35 (5) ◽  
pp. 1100-1106 ◽  
Author(s):  
Y. Kitahara ◽  
N. Suda ◽  
T. Terashima ◽  
O. Baba ◽  
K. Mekaapiruk ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Formation ◽  
Knockout Mice ◽  
Tooth Germ ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein ◽  
Tooth Germ Development

scholarly journals Increased Potential of Bone Formation with the Intravenous Injection of a Parathyroid Hormone-Related Protein Minicircle DNA Vector

2021 ◽  
Vol 22 (16) ◽  
pp. 9069
Author(s):  
Jang-Woon Kim ◽  
Narae Park ◽  
Jaewoo Kang ◽  
Yena Kim ◽  
Hyerin Jung ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Formation ◽  
Bone Structure ◽  
Human Mesenchymal Stem Cells ◽  
Treated Group ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein ◽  
Alternative Delivery ◽  
Minicircle Dna

Osteoporosis is commonly treated via the long-term usage of anti-osteoporotic agents; however, poor drug compliance and undesirable side effects limit their treatment efficacy. The parathyroid hormone-related protein (PTHrP) is essential for normal bone formation and remodeling; thus, may be used as an anti-osteoporotic agent. Here, we developed a platform for the delivery of a single peptide composed of two regions of the PTHrP protein (1–34 and 107–139); mcPTHrP 1–34+107–139 using a minicircle vector. We also transfected mcPTHrP 1–34+107–139 into human mesenchymal stem cells (MSCs) and generated Thru 1–34+107–139-producing engineered MSCs (eMSCs) as an alternative delivery system. Osteoporosis was induced in 12-week-old C57BL/6 female mice via ovariectomy. The ovariectomized (OVX) mice were then treated with the two systems; (1) mcPTHrP 1–34+107–139 was intravenously administered three times (once per week); (2) eMSCs were intraperitoneally administered twice (on weeks four and six). Compared with the control OVX mice, the mcPTHrP 1–34+107–139-treated group showed better trabecular bone structure quality, increased bone formation, and decreased bone resorption. Similar results were observed in the eMSCs-treated OVX mice. Altogether, these results provide experimental evidence to support the potential of delivering PTHrP 1–34+107–139 using the minicircle technology for the treatment of osteoporosis.

Measurement of Maternofetal Potential Difference in Parathyroid Hormone Related Protein (PTHrP) Knockout Mice

Placenta ◽  
2008 ◽  
Vol 29 (12) ◽  
pp. 1041-1042 ◽  
Author(s):  
M.R. Dilworth ◽  
J.D. Glazier ◽  
E.C. Cowley ◽  
R.D.H. Boyd ◽  
S.M. Husain ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Knockout Mice ◽  
Potential Difference ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein

Disturbed tooth development in parathyroid hormone-related protein (PTHrP)-gene knockout mice

Bone ◽  
2002 ◽  
Vol 30 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Y. Kitahara ◽  
N. Suda ◽  
T. Kuroda ◽  
F. Beck ◽  
V.E. Hammond ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Knockout Mice ◽  
Tooth Development ◽  
Gene Knockout ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein ◽  
Gene Knockout Mice

Biosynthesis of proteoglycan in bone and cartilage of parathyroid hormone-related protein knockout mice

2001 ◽  
Vol 19 (1) ◽  
pp. 4-12 ◽  
Author(s):  
Yuji Ogihara ◽  
Naoto Suda ◽  
Vicki E. Hammond ◽  
Paul V. Senior ◽  
Felix Beck ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Knockout Mice ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein ◽  
And Cartilage
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