scholarly journals Cryopreservation of Cell/Scaffold Tissue-Engineered Constructs

2012 ◽  
Vol 18 (11) ◽  
pp. 852-858 ◽  
Author(s):  
Pedro F. Costa ◽  
Ana F. Dias ◽  
Rui L. Reis ◽  
Manuela E. Gomes
Keyword(s):  
Cell Scaffold

scholarly journals Effects of an Acetic Acid and Acetone Mixture on the Characteristics and Scaffold–Cell Interaction of Electrospun Polycaprolactone Membranes

2019 ◽  
Vol 9 (20) ◽  
pp. 4350 ◽  
Author(s):  
Minh Hieu Ho ◽  
Thien Bui-Thuan Do ◽  
Nhi Ngoc-Thao Dang ◽  
An Nguyen-My Le ◽  
Hanh Thi-Kieu Ta ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Cell Interaction ◽  
Fibrous Materials ◽  
Cell Adherence ◽  
Physical Strength ◽  
Membrane Morphology ◽  
Cell Scaffold ◽  
Different Types ◽  
Proliferation Assays ◽  
Cell Adhesion And Proliferation

Green electrospinning has attracted great interest since non-toxic solvents were shown to be applicable in the fabrication of fibrous materials while ensuring health safety and environmental protection. Less harmful reagents such as acetone (AC) and acetic acid (AA) have been employed in this field in recent years. However, research in this area is still rare, yielding only preliminary results. In this study, two different types of solvents (pure AC and an AA/AC mixture) were used to fabricate electrospun polycaprolactone (PCL) membranes. Sample morphology, wettability, tensile strength, and chemical composition were compared between two types of membranes. Cell–scaffold interaction was also examined by cell adhesion and proliferation assays. The results demonstrate that the two types of solvents had significant effects on membrane morphology, physical strength, and cell adherence behaviors, which should be considered for different application purposes.

Development of high speed measurement system for cell-scaffold interaction

2015 ◽  
Author(s):  
Yu Hirano ◽  
Masaru Kojima ◽  
Mitsuhiro Horade ◽  
Kazuto Kamiyama ◽  
Yasushi Mae ◽  
...  
Keyword(s):  
Measurement System ◽  
High Speed ◽  
Speed Measurement ◽  
Cell Scaffold

Investigation of the Regenerative Potential of Human Bone Marrow Stem Cell-Seeded Polycaprolactone Bone Scaffolds, Fabricated Using Pneumatic Microextrusion Process

2021 ◽  
Author(s):  
Logan Lawrence ◽  
James B. Day ◽  
Pier Paolo Claudio ◽  
Roozbeh (Ross) Salary
Keyword(s):  
Stem Cell ◽  
Structural Integrity ◽  
Biological Tissues ◽  
Direct Write ◽  
Osteosarcoma Cell ◽  
Bone Scaffolds ◽  
Depth Analysis ◽  
Cell Scaffold ◽  
Material Process

Abstract Pneumatic MicroExtrusion (PME) is a direct-write additive manufacturing process, which has emerged as a robust, high-resolution method for the fabrication of a broad spectrum of biological tissues and organs. However, the PME process is intrinsically complex, governed by bio-physio-chemical phenomena as well as material-process interactions. Hence, investigation of the influence of consequential factors on bone scaffold fabrication as well as investigation of cell-scaffold interactions would be an inevitable need. The objective of the work is to investigate the biocompatibility as well as the histological properties of PME-fabricated porous bone scaffolds, composed of polycaprolactone (PCL). To achieve this objective, a media extraction of the scaffold material was tested for cytostatic or cytotoxic activity with the aim to: (i) assess the fabricated scaffolds’ feasibility of use in regenerative medicine, and (ii) determine their structural integrity in a modelled in-vivo environment. In addition, the scaffolds were inoculated with an established osteosarcoma cell line (SAOS-2) and cultured for seven days to investigate the scaffold architecture and cell integration potential. A histological examination was performed on the seeded scaffolds for further in-depth analysis of cell-scaffold interaction. Overall, the results of this study pave the way for future investigation of stem cell incorporation into PME-fabricated PCL scaffolds toward the treatment of osseous fractures and defects.

scholarly journals Bioassembly of three-dimensional embryonic stem cell-scaffold complexes using compressed gases

2009 ◽  
Vol 25 (2) ◽  
pp. 535-542 ◽  
Author(s):  
Yubing Xie ◽  
Yong Yang ◽  
Xihai Kang ◽  
Ruth Li ◽  
Leonithas I. Volakis ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Cell Scaffold

Proliferation and Adhesion of L929 Fibroblasts on Surface with Different Microtopography

2014 ◽  
Vol 1648 ◽  
Author(s):  
Yuta Kurashina ◽  
Shogo Miyata ◽  
Jun Komotori ◽  
Tadayoshi Koyama
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Low Frequency ◽  
Polished Surface ◽  
Stainless Steel Surface ◽  
Cell Counts ◽  
Adhesion Behavior ◽  
Cell Scaffold ◽  
Cell Adhesion And Proliferation ◽  
Number Of Cells

ABSTRACTThree types of 316L stainless steel surface with different topography were prepared by a Fine Particle Peening (FPP) treatment using titania, silica and alumina shot particles and analyzed the cell proliferation and cell-scaffold interaction. FPP-treated surface with titania and silica particles had micro asperities at low frequency. On the other hand, the alumina treated surface had micro asperities at high frequency. L929 fibroblasts were seeded on these specimens and then the number of cells was counted after 72 hours of culturing. The FPP-treated surfaces showed good cell proliferation comparing to polished surface. This indicates that micro asperities formed on the surface encourage cell adhesion. Cell adhesion behavior was evaluated by a scanning electron microscope (SEM) and a fluorescence microscope. Dense filopodia were observed when cells cultured on the FPP-treated surface. This means that FPP treatment enhances cell adhesion and proliferation. The number of cells observed on the FPP-treated surface depended on the shape of asperities formed by FPP treatment; the highest cell counts were obtained on alumina treated surface. This is because cell migration was not inhibited by the shape of alumina treated surface asperities.

Robust and hydrophilic polymeric films with honeycomb pattern and their cell scaffold applications

2009 ◽  
Vol 19 (18) ◽  
pp. 2789 ◽  
Author(s):  
Lei Li ◽  
Caikang Chen ◽  
Jian Li ◽  
Aijuan Zhang ◽  
Xinyu Liu ◽  
...  
Keyword(s):  
Polymeric Films ◽  
Cell Scaffold ◽  
Honeycomb Pattern

scholarly journals Injectable Hydrogel Combined with Nucleus Pulposus-Derived Mesenchymal Stem Cells for the Treatment of Degenerative Intervertebral Disc in Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-17 ◽  
Author(s):  
Feng Wang ◽  
Li-ping Nan ◽  
Shi-feng Zhou ◽  
Yang Liu ◽  
Ze-yu Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Rat Model ◽  
Injectable Hydrogel ◽  
Cell Scaffold ◽  
Ivd Degeneration

Stem cell-based tissue engineering in treating intervertebral disc (IVD) degeneration is promising. An appropriate cell scaffold can maintain the viability and function of transplanted cells. Injectable hydrogel has the potential to be an appropriate cell scaffold as it can mimic the condition of the natural extracellular matrix (ECM) of nucleus pulposus (NP) and provide binding sites for cells. This study was aimed at investigating the effect of injectable hydrogel-loaded NP-derived mesenchymal stem cells (NPMSC) for the treatment of IVD degeneration (IDD) in rats. In this study, we selected injectable 3D-RGD peptide-modified polysaccharide hydrogel as a cell transplantation scaffold. In vitro, the biocompatibility, microstructure, and induced differentiation effect on NPMSC of the hydrogel were studied. In vivo, the regenerative effect of hydrogel-loaded NPMSC on degenerated NP in a rat model was evaluated. The results showed that NPMSC was biocompatible and able to induce differentiation in hydrogel in vivo. The disc height index (almost 87%) and MRI index (3313.83±227.79) of the hydrogel-loaded NPMSC group were significantly higher than those of other groups at 8 weeks after injection. Histological staining and immunofluorescence showed that the hydrogel-loaded NPMSC also partly restored the structure and ECM content of degenerated NP after 8 weeks. Moreover, the hydrogel could support long-term NPMSC survival and decrease cell apoptosis rate of the rat IVD. In conclusion, injectable hydrogel-loaded NPMSC transplantation can delay the level of IDD and promote the regeneration of the degenerative IVD in the rat model.

scholarly journals Current Progress in Cross-Linked Peptide Self-Assemblies

2020 ◽  
Vol 21 (20) ◽  
pp. 7577
Author(s):  
Noriyuki Uchida ◽  
Takahiro Muraoka
Keyword(s):  
Self Assembly ◽  
Three Dimensional ◽  
Chemical Properties ◽  
Water Soluble ◽  
Cross Linking ◽  
Physical And Chemical Properties ◽  
Cell Scaffold ◽  
Scaffold Materials ◽  
Physical And Chemical ◽  
And Chemical Properties

Peptide-based fibrous supramolecular assemblies represent an emerging class of biomaterials that can realize various bioactivities and structures. Recently, a variety of peptide fibers with attractive functions have been designed together with the discovery of many peptide-based self-assembly units. Cross-linking of the peptide fibers is a key strategy to improve the functions of these materials. The cross-linking of peptide fibers forming three-dimensional networks in a dispersion can lead to changes in physical and chemical properties. Hydrogelation is a typical change caused by cross-linking, which makes it applicable to biomaterials such as cell scaffold materials. Cross-linking methods, which have been conventionally developed using water-soluble covalent polymers, are also useful in supramolecular peptide fibers. In the case of peptide fibers, unique cross-linking strategies can be designed by taking advantage of the functions of amino acids. This review focuses on the current progress in the design of cross-linked peptide fibers and their applications.

scholarly journals Dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3D cell culture

RSC Advances ◽  
2020 ◽  
Vol 10 (33) ◽  
pp. 19587-19599 ◽  
Author(s):  
Zhiping Fan ◽  
Ping Cheng ◽  
Min Liu ◽  
Sangeeta Prakash ◽  
Jun Han ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Drug Release ◽  
Release Rate ◽  
3D Cell Culture ◽  
Drug Release Rate ◽  
Sustained Drug Delivery ◽  
Cell Scaffold ◽  
Potential Applications

Polysaccharides-polypeptide derived biohydrogels were formed using hydrazone chemistry as crosslinking strategy, which have controllable drug release rate and many other potential applications, especially in sustained drug delivery and cell scaffold.

Sign in / Sign up

Export Citation Format

Share Document