BMP-2 Embedded Atelocollagen Scaffold for Tissue-Engineered Cartilage Cultured in the Medium Containing Insulin and Triiodothyronine—A New Protocol for Three-Dimensional In Vitro Culture of Human Chondrocytes

2012 ◽  
Vol 18 (5) ◽  
pp. 374-386 ◽  
Author(s):  
Edward Chengchuan Ko ◽  
Yuko Fujihara ◽  
Toru Ogasawara ◽  
Yukiyo Asawa ◽  
Satoru Nishizawa ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Three Dimensional ◽  
Human Chondrocytes ◽  
Engineered Cartilage

scholarly journals Reversal of senescence-associated beta-galactosidase expression during in vitro three-dimensional tissue-engineering of human chondrocytes in a polymer scaffold

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shojiro Katoh ◽  
Atsuki Fujimaru ◽  
Masaru Iwasaki ◽  
Hiroshi Yoshioka ◽  
Rajappa Senthilkumar ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Replicative Senescence ◽  
Cultured Cells ◽  
Three Dimensional ◽  
Human Chondrocytes ◽  
Cartilage Tissue ◽  
Optimal Method ◽  
Beta Galactosidase

AbstractRegenerative medicine applications require cells that are not inflicted with senescence after in vitro culture for an optimal in vivo outcome. Methods to overcome replicative senescence include genomic modifications which have their own disadvantages. We have evaluated a three-dimensional (3D) thermo-reversible gelation polymer (TGP) matrix environment for its capabilities to reverse cellular senescence. The expression of senescence-associated beta-galactosidase (SA-βgal) by human chondrocytes from osteoarthritis-affected cartilage tissue, grown in a conventional two-dimensional (2D) monolayer culture versus in 3D-TGP were compared. In 2D, the cells de-differentiated into fibroblasts, expressed higher SA-βgal and started degenerating at 25 days. SA-βgal levels decreased when the chondrocytes were transferred from the 2D to the 3D-TGP culture, with cells exhibiting a tissue-like growth until 42–45 days. Other senescence associated markers such as p16INK4a and p21 were also expressed only in 2D cultured cells but not in 3D-TGP tissue engineered cartilage. This is a first-of-its-kind report of a chemically synthesized and reproducible in vitro environment yielding an advantageous reversal of aging of human chondrocytes without any genomic modifications. The method is worth consideration as an optimal method for growing cells for regenerative medicine applications.

scholarly journals Beyond 3D culture models of cancer

2015 ◽  
Vol 7 (283) ◽  
pp. 283ps9-283ps9 ◽  
Author(s):  
Kandice Tanner ◽  
Michael M. Gottesman
Keyword(s):  
In Vitro Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Drug Efficacy ◽  
Spatiotemporal Evolution ◽  
Culture Models ◽  
Dynamic Interplay

The mechanisms underlying the spatiotemporal evolution of tumor ecosystems present a challenge in evaluating drug efficacy. In this Perspective, we address the use of three-dimensional in vitro culture models to delineate the dynamic interplay between the tumor and the host microenvironment in an effort to attain realistic platforms for assessing pharmaceutical efficacy in patients.

scholarly journals In vitro culture of hFOB1.19 osteoblast cells on TGF-β1-SF-CS three-dimensional scaffolds

2015 ◽  
Vol 13 (1) ◽  
pp. 181-187 ◽  
Author(s):  
SHUANG TONG ◽  
LEI XUE ◽  
DA-PENG XU ◽  
ZI-MEI LIU ◽  
YANG DU ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Three Dimensional ◽  
Osteoblast Cells ◽  
Tgf Β1

scholarly journals Cell migration and organization in three-dimensional in vitro culture driven by stiffness gradient

2016 ◽  
Vol 113 (11) ◽  
pp. 2496-2506 ◽  
Author(s):  
Danielle Joaquin ◽  
Michael Grigola ◽  
Gubeum Kwon ◽  
Christopher Blasius ◽  
Yutao Han ◽  
...  
Keyword(s):  
Cell Migration ◽  
In Vitro Culture ◽  
Three Dimensional

scholarly journals Functional properties of bone marrow-derived MSC-based engineered cartilage are unstable with very long-term in vitro culture

2014 ◽  
Vol 47 (9) ◽  
pp. 2173-2182 ◽  
Author(s):  
Megan J. Farrell ◽  
Matthew B. Fisher ◽  
Alice H. Huang ◽  
John I. Shin ◽  
Kimberly M. Farrell ◽  
...  
Keyword(s):  
Bone Marrow ◽  
In Vitro Culture ◽  
Functional Properties ◽  
Engineered Cartilage

scholarly journals Development of Spermatogenesis In Vitro in Three-Dimensional Culture from Spermatogonial Cells of Busulfan-Treated Immature Mice

2018 ◽  
Vol 19 (12) ◽  
pp. 3804 ◽  
Author(s):  
Ali AbuMadighem ◽  
Ronnie Solomon ◽  
Alina Stepanovsky ◽  
Joseph Kapelushnik ◽  
QingHua Shi ◽  
...  
Keyword(s):  
Male Infertility ◽  
In Vitro Culture ◽  
Seminiferous Tubule ◽  
Three Dimensional ◽  
Testicular Tissue ◽  
Biologically Active ◽  
Isolated Cells ◽  
Dead Box ◽  
Testicular Weight

Aggressive chemotherapy may lead to permanent male infertility. Prepubertal males do not generate sperm, but their testes do contain spermatogonial cells (SPGCs) that could be used for fertility preservation. In the present study, we examined the effect of busulfan (BU) on the SPGCs of immature mice, and the possible induction of the survivor SPGCs to develop spermatogenesis in 3D in-vitro culture. Immature mice were injected with BU, and after 0.5–12 weeks, their testes were weighed and evaluated histologically compared to the control mice. The spermatogonial cells [Sal-like protein 4 (SALL4) and VASA (a member of the DEAD box protein family) in the testicular tissue were counted/seminiferous tubule (ST). The cells from the STs were enzymatically isolated and cultured in vitro. Our results showed a significant decrease in the testicular weight of the BU-treated mice compared to the control. This was in parallel to a significant increase in the number of severely damaged STs, and a decrease in the number of SALL4 and VASA/STs compared to the control. The cultures of the isolated cells from the STs of the BU-treated mice showed a development of colonies and meiotic and post-meiotic cells after four weeks of culture. The addition of homogenates from adult GFP mice to those cultures induced the development of sperm-like cells after four weeks of culture. This is the first study demonstrating the presence of biologically active spermatogonial cells in the testicular tissue of BU-treated immature mice, and their capacity to develop sperm-like cells in vitro.

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